Preclinical evaluation of the polycaprolactone-polyethylene glycol electrospun nanofibers containing egg-yolk oil for acceleration of full thickness burns healing

Abstract

Considering the great potential of egg yolk oil (EYO) in management of burn wounds and superb biological properties of polycaprolactone (PCL) and polyethylene glycol (PEG), hereby, a PCL-PEG-EYO scaffold was developed by electrospinning method for burn healing. The physico-chemical characterizations were performed using SEM, FTIR and contact angle tests. The biological properties of the fabricated scaffolds were evaluated by antibacterial test, in vitro cell culturing, MTT assay and in vivo experiments. The SEM images of PCL-PEG-EYO nanofibers demonstrated a uniform bead-free morphology with 191 ± 61 nm diameter. The fabricated scaffold revealed hydrophilicity with the water contact angel of 77°. No cytotoxicity was observed up to 7 days after cell culturing onto the PCL-PEG-EYO nanofibrous surface. The presence of EYO in the PCL-PEG-EYO scaffold meaningfully improved the cell viability, proliferation and attachment compared to PCL-PEG scaffold. Moreover, the PCL-PEG-EYO scaffolds demonstrated antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa bacteria strain. Finally, a statistically significant enhancement in wound closure, re-epithelialization, angiogenesis and collagen synthesis was observed at the end of 21-day treatment period using PCL-PEG-EYO nanofibrous scaffold. Overall, the PCL-PEG-EYO nanofibrous scaffolds demonstrated a great potential in management of full thickness burn wounds in vivo.

Functional electrospun nanofibers: fabrication, properties, and applications in wound-healing process

“…Wound monitoring using electrospun nanofibers typically involves three components: sensors, drug delivery, and support structures. 23,103 When applied to wound monitoring, sensors can detect parameters such as wound temperature, humidity, and pH, and transmit these data to external devices for analysis and processing. 184,185 When applied to wound treatment, nanofibers can release drugs, promoting wound healing and preventing infection.…”

“…For example, they are used in water ltration treatment, 14 photocatalytic treatment of water pollution, 15 electrocatalysis, 16 and ltration in the eld of environmental protection. According to reports, there are already dozens of polymers that have been made into nanober structures through electrospinning technology, 17,18 such as poly-L-lactic acid (PLA), 19,20 polyvinyl alcohol (PVA), 21,22 polycaprolactone (PCL), [22][23][24][25] polyurethane (PU), 26 polyvinylidene uoride (PVDF), 27 and polyhydroxybutyric acid ester hydroxy valerate (PHBV), 28,29 which are also used in the development of electrospinning materials.…”

Electrospun Nanofiber Scaffold for Skin Tissue Engineering: A Review

“…27 The dressing for burn wounds must possess adequate breathability to facilitate optimal wound healing, as indicated by previous studies. 48,107 Adequate hydrophilicity can help to maintain the appropriate moisture levels in wounds as well as soften necrotic tissues. This property also enhances the ability to promote the regeneration of skin tissue.…”

Biomaterials for immunomodulation in wound healing

“…Studies have indicated that non-ionic hydrophilic surfaces can reduce the production of pro-inflammatory cytokines, indicating their potential to limit both leukocyte adhesion and macrophage fusion and promote regenerative inflammation [ 153 ]. Biomaterials such as poly(ethylene glycol) (PEG), HA, glycosaminoglycans, chitosan, heparin and phosphatidylcholine have been used to increase hydrophilicity and reduce protein adsorption through various processes [ 154–158 ]. Synthetic polymers such as poly(lactic acid), poly(lactic-co-glycolic acid) (PLGA) and poly(vinyl alcohol) have also shown potential for enhancing biocompatibility by limiting protein adsorption and consequently reducing NEU recruitment [ 159–161 ].…”