Preclinical evaluation of coronary vascular function after cardioplegia with HTK and different antioxidant additives

Abstract

Despite similar myocardial function and marker release, coronary vascular function after cardioplegic storage may profit by addition of iron chelators (or antioxidants) to traditional HTK solution.

Endothelial Dysfunction After Long-term Cold Storage in HTK Organ Preservation Solutions: Effects of Iron Chelators and N-α-acetyl-l-histidine

“…27,28 The aforementioned iron-dependent and -independent injury is not addressed by cardioplegic and organ preservation solutions currently in clinical use. As previously reported, 16 our data on the widely used Custodiol solution (Figures 1 and 3) clearly confirm the inability of this HTK solution to offer good protection and preservation of endothelial function after long-term cold ischemic storage.…”

“…10 Both cold storage and the amino acid histidine, a main component of the HTK solutions, induce an increased intracellular level of redox-active chelatable iron, [11][12][13] and thus the irondependent formation of ROS, mainly the highly toxic hydroxyl radicals. 14,15 Therefore, recent efforts have been made to reduce oxidative stress in various experimental models by pharmacologically chelating redoxactive iron, 13,16 using the traditional hydrophilic iron chelator, deferoxamine, and a newly developed lipophilic, membrane-permeable hydroxamic acid derivative, LK-614.…”

Even short-time storage in physiological saline solution impairs endothelial vascular function of saphenous vein grafts

“…Several studies have indicated the superiority of HTK solution or heparinized blood in comparison to the reported alternatives [16,17]. However, more recently, a poor preservation of HTK solution with respect to coronary artery function has been reported [20]. Despite these evaluations, storage with saline solutions still remains the clinical routine [16].…”

Cardioprotective effect of histidine-containing dipeptides in pharmacological cold cardioplegia

“…Infusion of custodiol and ACH CPS by the method described previously [7] arrested cardiac activity after 2.5-3 min, while St. Thomas Hospital hyperpotassium CPS arrested cardiac activity by the 4-th min of infusion. In series I, spontaneous recovery of heart work during minutes 6-7 of reperfusion was observed only in 2 cases.…”

“…The heart was fixed on a cannula of the isolated heart device. Perfusion was carried out by the Langendorff' method with Krebs-Henseleit solution at 37 o C [6,7], which was followed by a 15-min period of adaptation to antegrade perfusion. Perfusion was then stopped and CPS (12-14 o C) was infused for 5 min at a rate of 15-20 droplet/min.…”