Preclinical Development of Tissue-Engineered Vein Valves and Venous Substitutes using Re-Endothelialised Human Vein Matrix

Teebken, Omke E.; Puschmann, Carmen; Breitenbach, Ingo; Rohde, B; Burgwitz, Karin; Haverich, Axel

doi:10.1016/j.ejvs.2008.10.012

Cited by 31 publications

(21 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the past, decellularized blood vessels have been studied for their potential use as vascular grafts for small diameter arteries (Antonova et al, 2008;Borschel et al, 2005;Jo et al, 2007;Schaner et al, 2004;Gui and Niklason, 2014). They consist of a natural extracellular matrix, have very low immunogenicity and can be reseeded with various cells of choice and for any period of time (Teebken et al, 2009;Amiel et al, 2006). Though numerous decellularization protocols exist, there was no satisfying procedure for modeling the in vivo situation due to significantly altered mechanical characteristics compared to native vessels (Roy et al, 2005;McFetridge et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Development and characterization of an ex vivo arterial long-term proliferation model for restenosis research

Haase

Otto

Romeike

et al. 2015

ALTEX

View full text Add to dashboard Cite

SummaryOne of the main limitations of percutaneous coronary interventions is restenosis, occurring in small-diameter arteries. Many efforts are underway to find improved intracoronary devices to prevent in-stent restenosis. The aim of this study was to produce a new in vitro test platform for restenosis research, suitable for long-term cell proliferation and migration studies in stented vessels. Fresh segments of porcine coronary arteries were obtained for decellularization and were then reseeded with human coronary artery endothelial (HCAEC) and human coronary artery smooth muscle cells (HCASMC). Subsequently, bare metal stents (BMS) or drug eluting stents (DES) were implanted and the segments were reseeded with HCAEC and HCASMC for up to three months. The stented segments were examined at time zero and after 2, 4, 6, 8 and 12 weeks by histochemical and immunohistochemical characterization and the reseeded areas before and after stent implantation were measured. Cells formed multiple layers after three months and detection of both CD31 and α-smooth muscle actin by specific antibodies showed that HCAEC and HCASMC are adherent and growing in several layers. Furthermore, we could show a significantly smaller proliferation area in DES (70% ± 3.5%) compared to BMS (17% ± 2.3%). These data are similar to animal and human studies. Therefore, this vessel model might be suitable as an initial benchmark for testing new anti-proliferative endovascular therapies and could consequently help to reduce animal experiments in this research area.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Despite the existence of various decellularization protocols, e.g., for bladder (Bolland et al, 2007) and heart valves (Rieder et al, 2004;Teebken et al, 2009), the efficiency of a method depends mainly on the tissue type. Previously described protocols (Dahl et al, 2003;Williams et al, 2009) did not work properly for arterial vessels.…”

Section: A B Cmentioning

confidence: 99%

Development and characterization of an ex vivo arterial long-term proliferation model for restenosis research

Haase

Otto

Romeike

et al. 2015

ALTEX

View full text Add to dashboard Cite

show abstract

“…Venous endothelial cells or bone marrow-derived endothelial cells have demonstrated their affinity to the decellularized venous surface [Teebken et al, 2009;Shen et al, 2016]. Kuna et al [2015] introduced a model consisting of decellularized venous valves incubated with whole blood followed by selective perfusion with endothelial growth medium.…”

Section: Discussionmentioning

confidence: 99%

Tissue Engineering of Vein Valves Based on Decellularized Natural Matrices

Mogaldea

Goecke²,

Theodoridis³

et al. 2017

Cells Tissues Organs

View full text Add to dashboard Cite

Valvular repair or transplantation, designed to restore the venous valve function of the legs, has been proposed as treatment in chronic venous insufficiency. Available grafts or surgeries have provided limited durability so far. Generating venous valve substitutes by means of tissue engineering could be a solution. We generated decellularized jugular ovine vein conduits containing valves (oVVC) after reseeding with ovine endothelial cells differentiated from peripheral blood-derived endothelial cells (oPBEC), cultivated in vitro corresponding to the circulatory situation in the lower leg at rest and under exertion. oVVC were decellularized by detergent treatment. GFP-labeled oPBEC were seeded onto the luminal side of the decellularized oVVC and cultivated under static-rotational conditions for 6 h (group I) and 12 h (group II), respectively. Reseeded matrices of group I were exposed to continuous low flow conditions (“leg at rest”). The tissues of group II were exposed to a gradually increasing flow (“leg under effort”). After 5 days, the grafts of group I revealed a uniform luminal endothelial cell coverage of the examined areas of the venous walls and adjacent venous valve leaflets. In group II, the cell coverage on luminal areas of the venous wall parts was found to be nearly complete. The endothelial cell coverage of adjacent venous valve leaflets was revealed to be less dense and confluent. Endothelial cells cultured on acellular vein tissues of both groups were distinctly orientated uniformly in the flow direction, clearly creating a stable and flow-orientated layer. Thus, an endothelium could successfully be reestablished on the luminal surface of a decellularized venous valve by seeding peripheral blood endothelial cells and culturing under different conditions.

show abstract

“…133,134 While these systems provide insight into cellular behavior in these more complex conditions, they are less conducive to vessel fabrication in vitro . Still more complex bioreactors incorporate perfused, harvested vessels or vessel constructs; engineered blood vessels mimics such as decellularized cadaver vessels, 135 porcine arteries, 136 and human saphenous veins 137 ; peripheral blood–derived blood vessel mimics 138,139 ; and synthetic cylindrical polymer scaffolds 140 to generate vessels as small as 4 mm in diameter.…”

Section: Microvascular Assemblymentioning

confidence: 99%

Manipulating the Microvasculature and Its Microenvironment

Krishnan

Chang

Nunes

et al. 2013

Crit Rev Biomed Eng

View full text Add to dashboard Cite

The microvasculature is a dynamic cellular system necessary for tissue health and function. Therapeutic strategies that target the microvasculature are expanding and evolving, including those promoting angiogenesis and microvascular expansion. When considering how to manipulate angiogenesis, either as part of a tissue construction approach or a therapy to improve tissue blood flow, it is important to know the microenvironmental factors that regulate and direct neovessel sprouting and growth. Much is known concerning both diffusible and matrix-bound angiogenic factors, which stimulate and guide angiogenic activity. How the other aspects of the extravascular microenvironment, including tissue biomechanics and structure, influence new vessel formation is less well known. Recent research, however, is providing new insights into these mechanisms and demonstrating that the extent and character of angiogenesis (and the resulting new microcirculation) is significantly affected. These observations and the resulting implications with respect to tissue construction and microvascular therapy are addressed.

show abstract

Preclinical Development of Tissue-Engineered Vein Valves and Venous Substitutes using Re-Endothelialised Human Vein Matrix

Cited by 31 publications

References 34 publications

Development and characterization of an ex vivo arterial long-term proliferation model for restenosis research

Development and characterization of an ex vivo arterial long-term proliferation model for restenosis research

Tissue Engineering of Vein Valves Based on Decellularized Natural Matrices

Manipulating the Microvasculature and Its Microenvironment

Contact Info

Product

Resources

About