“…The animals' blood samples were drawn and stored in BD Vacutainer, Fisher Scientific Inc. (Schwerte, Germany). 68 Ga]Ga-PSMA-617 and [ 68 Ga]Ga-PSMA-617-ME The principle of the radiosynthesis for [ 68 Ga]Ga -PSMA-617 and formulation into the water-in-oil-in-water (w/o/w) microemulsion was adopted from a previously described method [11]. Briefly, the generator-derived 68 Ga-radioactivity was eluted by performing manual eluate fractionation as described by Breeman et al, 2005 [23] and measured in a dose calibrator (CRC15, Capintec Inc, Pittsburgh, PA, USA) using 0.6 M hydrochloric acid Sigma Aldrich (St Louis, MO, USA).…”