Preclinical anti-inflammatory and antioxidant effects of Azanza garckeana in STZ-induced glycemic-impaired rats, and pharmacoinformatics of it major phytoconstituents

Lawal, Bashir; Sani, Saidu; Onikanni, Sunday Amos; Ibrahim, Yunusa Olatunji; Agboola, Abdulhakeem Rotimi; Lukman, Halimat Yusuf; Olawale, Femi; Jigam, Ali A.; Batiha, Gaber El‐Saber; Babalola, Shukurat Bisola; Mostafa‐Hedeab, Gomaa; Lima, Clara Mariana Gonçalves; Wu, Alexander T.H.; Huang, Hsu-Shan; Conté-Júnior, Carlos Adam

doi:10.1016/j.biopha.2022.113196

Cited by 23 publications

(15 citation statements)

References 63 publications

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“…Determining the PK properties of a drug is an integral part of early drug discovery and development. It is an important feature that defines the clinical success of a therapeutic agent [ 89 , 90 ]. Many drugs fail in clinical trials due to poor PKs [ [91] , [92] , [93] ].…”

Section: Resultsmentioning

confidence: 99%

Identification of a novel immune-inflammatory signature of COVID-19 infections, and evaluation of pharmacokinetics and therapeutic potential of RXn-02, a novel small-molecule derivative of quinolone

Lawal

Kuo

Sumitra

et al. 2022

Computers in Biology and Medicine

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Section: Resultsmentioning

confidence: 99%

Identification of a novel immune-inflammatory signature of COVID-19 infections, and evaluation of pharmacokinetics and therapeutic potential of RXn-02, a novel small-molecule derivative of quinolone

Lawal

Kuo

Sumitra

et al. 2022

Computers in Biology and Medicine

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“…The dietl-eth_ T. garckeana was evaluated for its in vivo anti-inflammatory effects in mice using a xylene-induced ear edema test [ 45 , 46 ]. Healthy mice were divided into five ( n = 5) groups and administered orally with saline, dietl-eth_ T. garckeana (150 and 300 mg/kg BW), or 200 mg/kg aspirin for 7 days.…”

Section: Methodsmentioning

confidence: 99%

Luteolin-Rich Extract of Thespesia garckeana F. Hoffm. (Snot Apple) Contains Potential Drug-Like Candidates and Modulates Glycemic and Oxidoinflammatory Aberrations in Experimental Animals

Alozieuwa

Lawal

Sani³

et al. 2022

Oxidative Medicine and Cellular Longevity

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The present study evaluated the polyphenolic contents and hypoglycemic, antioxidant, and anti-inflammatory effects of the diethyl ether fraction of Thespesia garckeana using various in vitro and in vivo models. Total phenol and flavonoid contents of the extract were 613.65 ± 2.38 and 152.83 ± 1.56 mg/100 g dry weight, respectively. The extract exhibited in vitro antioxidant activities against DPPH, FRAP, LPO, and ABTS with respective half-maximal inhibitory concentration (IC50) values of 30.91 ± 0.23 , 16.81 ± 0.51 , 41.29 ± 1.82 , and 42.39 ± 2.24 μg/mL. In vitro anti-inflammatory studies using membrane stabilization, protein denaturation, and proteinase activities revealed the effectiveness of the extract with respective IC50 values of 54.45 ± 2.89 , 93.62 ± 3.04 , and 56.60 ± 2.34 μg/mL, while in vitro hypoglycemic analysis of the extract revealed inhibition of α-amylase (IC50 64.59 ± 3.29 μg/mL) and enhancement of glucose uptake by yeast cells. Interestingly, the extract demonstrated in vivo hypoglycemic and anti-inflammatory effects in streptozotocin- (STZ-) induced diabetic and xylene-induced ear swelling models, respectively. In addition, the extract improved insulin secretion, attenuated pancreatic tissue distortion and oxidative stress, and increased the activities of superoxide dismutase (SOD), catalase, and reduced glutathione (GSH), while reducing the concentration of LPO in the diabetic rats. A high-performance liquid chromatography (HPLC) analysis identified the presence of catechin ( 6.81 e − 1 ppm), rutin ( 8.46 e − 1 ppm), myricetin, apigenin ( 4.019 e − 1 ppm), and luteolin (15.09 ppm) with respective retention times (RTs) of 13.64, 24.269, 27.781, 29.58, and 32.23 min, and these were subjected to a pharmacoinformatics analysis, which revealed their drug-likeness and good pharmacokinetic properties. A docking analysis hinted at the potential of luteolin, the most abundant compound in the extract, for targeting glucose-metabolizing enzymes. Thus, the present study provides preclinical insights into the bioactive constituents of T. garckeana, its antioxidant and anti-inflammatory effects, and its potential for the treatment of diabetes.

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“…For example, the fruit contained a high amount of phenolic, tannin, flavonoid, and flavanol content, as shown in (Table 2). In contrast, Lawal et al [34] reported that the fruit pulp of jakjak contained about 260.80 ± 2.23 mg/100 g of total phenol and 10.28 ± 1.29 mg/100 g of total flavonoid content. The results of this study were lower than our findings; this variation might be due to the plant environment, fruit collection, and handling process.…”

Section: Phenolic Compounds Of Jakjak Fruitmentioning

confidence: 86%

Screening and Estimation of Bioactive Compounds of Azanza garckeana (Jakjak) Fruit Using GC-MS, UV–Visible Spectroscopy, and HPLC Analysis

et al. 2022

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Azanza garckeana (F. Hoffm). Exell and Hillc. is an important food and medicinal plant that has been used in tropical Africa. Therefore, this study aimed to investigate the nutritional value of jakjak fruit using different analytical techniques. The obtained results have demonstrated that jakjak fruit is very rich in total soluble sugar, constituting about 48% of the dry weight. Moreover, the chromatographic analysis revealed that jakjak fruit contained a high amount of glucose, fructose, maltose, and ascorbic acid. Further, GC-MS analysis detected four compounds related to secondary metabolites. Some of these detected constituents have medicinal value. For example, phenol, 2,4-bis (1,1-dimethylethyl) has been reported to have many functions such as antioxidant activity, anticancer, antifungal, and antibacterial properties. Furthermore, the antioxidant potential of different concentrations of deionized water and methanolic extracts was estimated using 2,2-diphenyl-1-picrylhydrazyl (DPPH). The results showed that the scavenging activity of the DPPH radical was found to be raised with increasing concentrations of fruit extracts. The concentration (50%) of both methanol and deionized water gave the best inhibition percentage (91.7 and 84.4%), respectively. In contrast, the methanolic extract has shown significant results compared to deionized water. This study concluded that jakjak fruit is very rich in total soluble sugar and phenolic compounds, which can be used as a source of polysaccharides and antioxidants for the human diet as well as raw materials for downstream industries.

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Preclinical anti-inflammatory and antioxidant effects of Azanza garckeana in STZ-induced glycemic-impaired rats, and pharmacoinformatics of it major phytoconstituents

Cited by 23 publications

References 63 publications

Identification of a novel immune-inflammatory signature of COVID-19 infections, and evaluation of pharmacokinetics and therapeutic potential of RXn-02, a novel small-molecule derivative of quinolone

Identification of a novel immune-inflammatory signature of COVID-19 infections, and evaluation of pharmacokinetics and therapeutic potential of RXn-02, a novel small-molecule derivative of quinolone

Luteolin-Rich Extract of Thespesia garckeana F. Hoffm. (Snot Apple) Contains Potential Drug-Like Candidates and Modulates Glycemic and Oxidoinflammatory Aberrations in Experimental Animals

Screening and Estimation of Bioactive Compounds of Azanza garckeana (Jakjak) Fruit Using GC-MS, UV–Visible Spectroscopy, and HPLC Analysis

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