Precision-porous polyurethane elastomers engineered for application in pro-healing vascular grafts: Synthesis, fabrication and detailed biocompatibility assessment

Zhen, Le; Creason, Sharon A.; Simonovsky, Felix I.; Snyder, Jessica M.; Lindhartsen, Sarah L.; Mecwan, Marvin; Johnson, Brian W.; Himmelfarb, Jonathan; Ratner, Buddy D.

doi:10.1016/j.biomaterials.2021.121174

Cited by 22 publications

(33 citation statements)

References 63 publications

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“…Artificial vascular grafts with enough mechanical strength can resist blood pressure continuously [ 28 , 29 , 30 , 31 ]. In the radial direction, the maximum stress, breaking strain, and modulus of TVGs were comparable to WSGs, while largely higher than those of ESGs, mainly due to the existence of circumferentially aligned fibers ( Figure 3 a–d).…”

Section: Resultsmentioning

confidence: 99%

Tri-Layered Vascular Grafts Guide Vascular Cells’ Native-like Arrangement

Yuan

Yao

et al. 2022

Polymers

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Bionic grafts hold great promise for directing tissue regeneration. In vascular tissue engineering, although a large number of synthetic grafts have been constructed, these substitutes only partially recapitulated the tri-layered structure of native arteries. Synthetic polymers such as poly(l-lactide-co-ε-caprolactone) (PLCL) possess good biocompatibility, controllable degradation, remarkable processability, and sufficient mechanical strength. These properties of PLCL show great promise for fabricating synthetic vascular substitutes. Here, tri-layered PLCL vascular grafts (TVGs) composed of a smooth inner layer, circumferentially aligned fibrous middle layer, and randomly distributed fibrous outer layer were prepared by sequentially using ink printing, wet spinning, and electrospinning techniques. TVGs possessed kink resistance and sufficient mechanical properties (tensile strength, elastic modulus, suture retention strength, and burst pressure) equivalent to the gold standard conduits of clinical application, i.e., human saphenous veins and human internal mammary arteries. The stratified structure of TVGs exhibited a visible guiding effect on specific vascular cells including enhancing endothelial cell (EC) monolayer formation, favoring vascular smooth muscle cells’ (VSMCs) arrangement and elongation, and facilitating fibroblasts’ proliferation and junction establishment. Our research provides a new avenue for designing synthetic vascular grafts with polymers.

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Section: Resultsmentioning

confidence: 99%

Tri-Layered Vascular Grafts Guide Vascular Cells’ Native-like Arrangement

Yuan

Yao

et al. 2022

Polymers

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“…In parallel with the development of scaffold optimization tools, equally important is a rich availability of scaffold fabrication techniques and biopolymer choices, both of which promote parametric control for optimizations. Some recent developments in the graft fabrication include: 1) Shortening the production time for cell sheet self-assembly method ( von Bornstädt et al, 2018 ); 2) loading drugs, anti-thrombogenic or pro-regenerative molecules for electrospun grafts or 3D printed grafts ( Zhang et al, 2019 ; Domínguez-Robles et al, 2021 ); 3) refining decellularization protocols for reduced immunological responses ( Schneider et al, 2018 ; Valencia-Rivero et al, 2019 ; Kimicata et al, 2020 ; Lopera Higuita et al, 2021 ); 4) improving the precision of pore generation in scaffold ( Zhen et al, 2021 ); 5) enhancing recellularization for allogenic or xenogenic decellularized grafts ( Dahan et al, 2017 ; Lin et al, 2019 ; Fayon et al, 2021 ); 6) expediting degradation with scaffold composition ( Fukunishi et al, 2021 ) or textile technique ( Fukunishi et al, 2019 ) to enhance matrix remodeling; 7) mimicking the structure and/or composition of vascular ECM using electrochemical fabrication ( Nguyen et al, 2018 ) or an automated technology combining dip-spinning with solution blow spinning ( Akentjew et al, 2019 ); 8) creating patient-specific grafts ( Fukunishi et al, 2017 ); and 9) hybrid approaches, for example, combining electrospinning with decellularized matrices ( Gong et al, 2016 ; Ran et al, 2019 ; Wu et al, 2019 ; Yang et al, 2019 ).…”

Section: Counteracting Adverse Remodeling With Regenerative Signalsmentioning

confidence: 99%

Strategies to counteract adverse remodeling of vascular graft: A 3D view of current graft innovations

Tan

Boodagh

Parthiban

et al. 2023

Front. Bioeng. Biotechnol.

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Vascular grafts are widely used for vascular surgeries, to bypass a diseased artery or function as a vascular access for hemodialysis. Bioengineered or tissue-engineered vascular grafts have long been envisioned to take the place of bioinert synthetic grafts and even vein grafts under certain clinical circumstances. However, host responses to a graft device induce adverse remodeling, to varied degrees depending on the graft property and host’s developmental and health conditions. This in turn leads to invention or failure. Herein, we have mapped out the relationship between the design constraints and outcomes for vascular grafts, by analyzing impairment factors involved in the adverse graft remodeling. Strategies to tackle these impairment factors and counteract adverse healing are then summarized by outlining the research landscape of graft innovations in three dimensions—cell technology, scaffold technology and graft translation. Such a comprehensive view of cell and scaffold technological innovations in the translational context may benefit the future advancements in vascular grafts. From this perspective, we conclude the review with recommendations for future design endeavors.

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“…In addition to the mechanical dynamics, material features such as the structure and morphology of the scaffolds have received increasing attention for tissue regeneration [ [7] , [8] , [9] , [10] ]. Pore sizes within the implanted material have been accepted as an important factor influencing macrophage polarization, which further regulate tissue regeneration including vascular remodeling [ 11 ].…”

Section: Introductionmentioning

confidence: 99%