Precision phenotyping of contrasting potato (Solanum tuberosum L.) varieties in a novel aeroponics system for improving nitrogen use efficiency: In search of key traits and genes

Genome-wide identification and characterization of microRNAs by small RNA sequencing for low nitrogen stress in potato

“…An Indian potato variety Kufri Jyoti (KJ) was used in this study, which is N inefficient [46]. Healthy in vitro plants were routinely maintained at Indian Council of Agricultural Research-Central Potato Research Institute, Shimla (31.1048˚N, 77.1734˚E 2,276 m above mean sea level), Himachal Pradesh, India.…”

“…Healthy in vitro plants were routinely maintained at Indian Council of Agricultural Research-Central Potato Research Institute, Shimla (31.1048˚N, 77.1734˚E 2,276 m above mean sea level), Himachal Pradesh, India. In vitro plants were grown in aeroponic till full crop cycle (110 days) with two nitrogen treatments [High N (HN): 25 milli molar N; and Low N (LN): 1 milli molar N] using both nitrate and ammonium form of N under controlled conditions supplied with all major and minor nutrients solution (except N) as described by Tiwari and coworkers [46] (Fig 1). Ammonium form of N was limited to 0.5 mM in both the treatments.…”

Prospects of Improving Nitrogen Use Efficiency in Potato: Lessons From Transgenics to Genome Editing Strategies in Plants

“…Moreover, the underlying molecular and physiological mechanisms and genetic factors remain elusive in potato for root system architecture, carbon-nitrogen economy and N metabolism (uptake, transport, utilization and remobilization). Recently, we have reviewed application of integrated genomics, physiology and breeding approaches for improving NUE (Tiwari et al, 2018 ) and traits phenotyping under aeroponic in potato (Tiwari et al, 2020d ). Further, recent studies provide information about the genes and miRNAs associated with N stress in potato (Tiwari et al, 2020a , b , c ; Zhang et al, 2020 ).…”

l-Aspartate: An Essential Metabolite for Plant Growth and Stress Acclimation

“…When carbon skeletons are limited, Asp is amidated to form Asn, which serves as an efficient nitrogen transport and storage compound due to its relatively high N:C ratio (2:4) [ 69 , 70 ]. Under nitrogen stress, Asp appears to be one of the most importantamino acids [ 71 , 72 , 73 , 74 , 75 ]. It has been found that when N is sufficient, as a predominant amino acid translocated in plant phloem, Asp supplied by the phloem is converted in the root to Asn to export N to the shoot via xylem as part of the process of nitrogen assimilation, whereas, when N is absent, Asp supplied by the phloem is diverted to the formation of malate to support the metabolism cycle back to the shoot [ 74 ].…”

“…It has been found that when N is sufficient, as a predominant amino acid translocated in plant phloem, Asp supplied by the phloem is converted in the root to Asn to export N to the shoot via xylem as part of the process of nitrogen assimilation, whereas, when N is absent, Asp supplied by the phloem is diverted to the formation of malate to support the metabolism cycle back to the shoot [ 74 ]. In a very recent study, higher Asp and Asn contents were observed to be positively coordinated with the nitrogen use efficiency (NUE) trait in potatoes with low N supply [ 75 ]. The above results suggest that Asp is imperative for amino acid and organic acid biosynthesis, especially under fluctuating N conditions.…”