Precision Microbiome Modulation with Discrete Dietary Fiber Structures Directs Short-Chain Fatty Acid Production

Deehan, Edward C.; Yang, Czau‐Siung; Pérez-Muñoz, María Elisa; Nguyen, Nguyen K.; Cheng, Chu; Triador, Lucila; Zhang, Zhengxiao; Bakal, Jeffrey A.; Walter, Jens

doi:10.1016/j.chom.2020.01.006

Cited by 356 publications

(320 citation statements)

References 99 publications

(126 reference statements)

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“…RS4 is a type of chemically modified starch that is not naturally occurring. In this study, we did not observe significant protein changes induced by RS4, which is similar to a recent study reporting that an RS4 did not affect the microbiome [41].…”

Section: Discussionsupporting

confidence: 92%

Metaproteomic responses ofin vitrogut microbiomes to resistant starches: the role of resistant starch type and inter-individual variations

Ryan

Ning

et al. 2020

Preprint

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Resistant starches (RS) are dietary compounds processed by the gut microbiota into metabolites, such as butyrate, that are beneficial to the host. The production of butyrate by the microbiome appears to be affected by the plant source and type of RS as well as the individual's microbiota. In this study, we used in vitro culture and metaproteomic methods to explore the consistency and variations in individual microbiome's functional responses to three types of RS -RS2(Hi Maize 260), RS3(Novelose 330) and RS4(Fibersym RW). Results showed that RS2 and RS3 significantly altered the levels of protein expression in the individual gut microbiomes, while RS4 did not result in significant protein changes. Significantly elevated protein groups were enriched in carbohydrate metabolism and transport functions of families Eubacteriaceae, Lachnospiraceae and Ruminococcaceae. In addition, Bifidobacteriaceae was significantly increased in response to RS3.We also observed taxon-specific enrichments of starch metabolism and pentose phosphate pathways corresponding to this family. Functions related to starch utilization, ABC transporters and pyruvate metabolism pathways were consistently increased in the individual microbiomes in response to RS2 and RS3; in contrast, the downstream butyrate producing pathway response varied. Our study confirm that different types of RS have markedly variable functional effects on the human gut microbiome, and also found considerable inter-individual differences in microbiome pathway responses.

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Section: Discussionsupporting

confidence: 92%

Metaproteomic responses ofin vitrogut microbiomes to resistant starches: the role of resistant starch type and inter-individual variations

Ryan

Ning

et al. 2020

Preprint

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“…Thus, it is unclear from our data how RPS supports the growth of Parasutterella. It is intriguing to note that Parasutterella was identi ed as part of a co-abundance response group that increased in response to chemically modi ed resistant starches (Type 4) in people [25]. This suggests that resistant starch may generally support the growth of Parasutterella, through cross-feeding or some other indirect mechanism(s).…”

Section: Discussionmentioning

confidence: 99%

Increasing levels of Parasutterella in the gut microbiome correlate with improving Low-Density Lipoprotein levels in healthy adults consuming resistant potato starch during a randomised trial

Bush

Alfa

2020

Preprint

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Background Prebiotics, defined as a substrate that is selectively utilized by host microorganisms conferring a health benefit, present a potential option to optimize gut microbiome health. Elucidating the relationship between specific intestinal bacteria, prebiotic intake, and the health of the host remains a primary microbiome research goal. Objective To assess the correlations between gut microbiota, serum health parameters, and prebiotic consumption in healthy adults. Methods We performed ad hoc exploratory analysis of changes in abundance of genera in the gut microbiome of 75 participants from a randomized, placebo-controlled clinical trial that evaluated the effects of resistant potato starch (RPS; MSPrebiotic®, N = 38) intervention versus a fully digestible placebo (N = 37) for which primary and secondary outcomes have previously been published. Pearson correlation analysis was used to identify relationships between health parameters (ie. blood glucose and lipids) and populations of gut bacteria. Results Abundance of Parasutterella (phylum Proteobacteria) tended to increase in the gut microbiome of individuals consuming RPS and those increases in Parasutterella were correlated with reductions in low-density lipoprotein (LDL) levels in participants consuming RPS but not placebo. Segregating RPS-consuming individuals whose LDL levels decreased (ie “Responders”) from those who did not (ie. “Non-Responders”) revealed that LDL Responders had significantly higher levels of Parasutterella both at baseline and after 12 weeks of consuming RPS. Conclusion Our analyses suggest that RPS may help improve LDL levels depending upon the levels of Parasutterella in an individual’s gut microbiome. Trial Registration: This study protocol was reviewed and approved by Health Canada (Submission #188517; “Notice of Authorization” dated 06/05/13) and registered as NCT01977183 (10/11/13) listed on NIH website: ClinicalTrials.gov. Data generated in this study have been submitted to NCBI (http://www.ncbi.nlm.nih.gov/bioproject/381931).

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“…64 These findings are in line with the clinical benefit observed with enteral nutrition regarding aGvHD and BSI. 65,66 The role of specific nutritional supplements aimed at promoting a GM mutualistic layout, for example, discrete fiber structures to boost directed SCFA production, 67 has not been explored yet in children undergoing HSCT, but has shown promising preliminary results in adults. 68…”

Section: Could Nutrition Be An Effective Way Of Modulating the Gm?mentioning

confidence: 99%

The gut microbiome in pediatric patients undergoing allogeneic hematopoietic stem cell transplantation

Masetti

Zama

Leardini

et al. 2020

Pediatric Blood & Cancer

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The gut microbiome (GM) has been associated with different clinical outcomes in the context of allogeneic hematopoietic stem cell transplantation (HSCT). Large multicenter cohort studies in adults have found significant correlations with overall survival, relapse, and incidence of complications. Moreover, GM is already a promising target for therapeutic interventions. However, few data are available in children, a population presenting unique features and challenges. During childhood, the GM evolves rapidly with large structural fluctuations, alongside with the maturation of the immune system. Furthermore, the HSCT procedure presents significant differences in children. These considerations underline the importance of a specific focus on the pediatric setting, and the role of GM and its age-dependent trajectory in influencing the immunity reconstitution and clinical outcomes. This review provides a comprehensive overview of the available evidence in the field of GM and pediatric HSCT, highlighting age-specific issues and discussing GM-based therapeutic approaches.

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Precision Microbiome Modulation with Discrete Dietary Fiber Structures Directs Short-Chain Fatty Acid Production

Cited by 356 publications

References 99 publications

Metaproteomic responses ofin vitrogut microbiomes to resistant starches: the role of resistant starch type and inter-individual variations

Metaproteomic responses ofin vitrogut microbiomes to resistant starches: the role of resistant starch type and inter-individual variations

Increasing levels of Parasutterella in the gut microbiome correlate with improving Low-Density Lipoprotein levels in healthy adults consuming resistant potato starch during a randomised trial

The gut microbiome in pediatric patients undergoing allogeneic hematopoietic stem cell transplantation

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