Precision genome engineering in rice using prime editing system

Hua, Kai; Jiang, Yuwei; Tao, Xiaoping; Zhu, Jian‐Kang

doi:10.1111/pbi.13395

Cited by 122 publications

(84 citation statements)

References 11 publications

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“…Very recently, several reports have shown that prime editing can be applied to cereal plants Lin et al, 2020;Tang et al, 2020;Xu et al, 2020a;Xu et al, 2020b;Butt et al;Hua et al). All these studies showed that the efficiency of PPEs greatly varies between target sites and is sensitive to several parameters such as both the PBS and RT sequence.…”

Section: Resultsmentioning

confidence: 99%

Prime editing is achievable in the tetraploid potato, but needs improvement

Veillet

Kermarrec

Chauvin

et al. 2020

Preprint

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Section: Resultsmentioning

confidence: 99%

Prime editing is achievable in the tetraploid potato, but needs improvement

Veillet

Kermarrec

Chauvin

et al. 2020

Preprint

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“…Meanwhile, Wei group reported that PE2 could generate 0%–31.3% editing frequency in T0 plants HPT −ATG reporter in rice while the PE3 systems did not show preferential editing efficiency [ 121 ]. Apart from this, Zhu and his group replaced SpCas9(H840A) with SaCas9(N580A) to enhance PE3 efficiency, but Sa-PE3 exhibited a lower editing activity compared to Sp-PE3 [ 122 ].…”

Section: Dsb and Template-free Editing In Plantsmentioning

confidence: 99%

CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants

Abdullah

Jiang

Hong

et al. 2020

Synthetic and Systems Biotechnology

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CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR associated) has been extensively exploited as a genetic tool for genome editing. The RNA guided Cas nucleases generate DNA double-strand break (DSB), triggering cellular repair systems mainly Non-homologous end-joining (NHEJ, imprecise repair) or Homology-directed repair (HDR, precise repair). However, DSB typically leads to unexpected DNA changes and lethality in some organisms. The establishment of bacteria and plants into major bio-production platforms require efficient and precise editing tools. Hence, in this review, we focus on the non-DSB and template-free genome editing, i.e., base editing (BE) and prime editing (PE) in bacteria and plants. We first highlight the development of base and prime editors and summarize their studies in bacteria and plants. We then discuss current and future applications of BE/PE in synthetic biology, crop improvement, evolutionary engineering, and metabolic engineering. Lastly, we critically consider the challenges and prospects of BE/PE in PAM specificity, editing efficiency, off-targeting, sequence specification, and editing window.

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“…Recently, a novel and universal precision genome-editing technology named prime editing was developed and tested in mammalian cells [1]. More recently, seven groups reported applications of prime editors (PE) in rice and wheat, and six groups achieved prime-edited rice lines [2][3][4][5][6][7][8]. Excluding the data based on enriching strategies or that are unsuitable for comparison, a total of 164 prime-edited rice lines transformed with 39 prime editors targeting 11 endogenous rice genes were generated in these previous reports [2,3,5,6,8].…”

Section: Introductionmentioning

confidence: 99%

“…More recently, seven groups reported applications of prime editors (PE) in rice and wheat, and six groups achieved prime-edited rice lines [2][3][4][5][6][7][8]. Excluding the data based on enriching strategies or that are unsuitable for comparison, a total of 164 prime-edited rice lines transformed with 39 prime editors targeting 11 endogenous rice genes were generated in these previous reports [2,3,5,6,8]. The highest editing efficiencies achieved by the five groups ranged from 2.22% to 31.3% [2,3,5,6,8].…”

Section: Introductionmentioning

confidence: 99%

“…Excluding the data based on enriching strategies or that are unsuitable for comparison, a total of 164 prime-edited rice lines transformed with 39 prime editors targeting 11 endogenous rice genes were generated in these previous reports [2,3,5,6,8]. The highest editing efficiencies achieved by the five groups ranged from 2.22% to 31.3% [2,3,5,6,8]. Of the total 164 primeedited rice lines, only one line, generated by Xu et al [6], harbors homozygous mutations; all the other lines mainly harbor chimeric mutations.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

Jiang

Chai

et al. 2020

Preprint

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AbstractA novel and universal CRISPR/Cas-derived precision genome editing technology named prime editing was developed. However, low prime editing efficiency was shown in transgenic rice lines. We reasoned that enhancing pegRNA expression would be able to improve prime editing efficiency. In this report, we used two strategies to enhance pegRNA expression and constructed a prime editing vector harboring two pegRNA variants for W542L and S621I double mutations in ZmALS1 and ZmALS2. As compared with previous reports in rice, we achieved much higher prime editing efficiency in maize. Our results are inspiring and provide a direction for optimization of plant prime editors.

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Precision genome engineering in rice using prime editing system

Cited by 122 publications

References 11 publications

Prime editing is achievable in the tetraploid potato, but needs improvement

Prime editing is achievable in the tetraploid potato, but needs improvement

CRISPR base editing and prime editing: DSB and template-free editing systems for bacteria and plants

Prime editing efficiently generates W542L and S621I double mutations in two ALS genes in maize

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