Precision-cut intestinal slices as a culture system to analyze the infection of differentiated intestinal epithelial cells by avian influenza viruses

Punyadarsaniya, Darsaniya; Winter, Christine; Mork, Ann Kathrin; Amiri, Mahdi; Naim, Hassan Y.; Rautenschlein, Silke; Herrler, Georg

doi:10.1016/j.jviromet.2014.10.015

Cited by 11 publications

(12 citation statements)

References 19 publications

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“…Previous studies have confirmed the importance of broiler breed to the final inflammatory response and suggest that cells from commercial fast and slower growing breeds may be more relevant to study avian gut responses ( Kaiser et al, 2016 ). Indeed, recent technologies point to the precision modeling of chicken intestinal slices ( Punyadarsaniya et al, 2015 ). Secondly, with respect to assay methodology, we recognize that 6 h is probably not the optimal time to measure adhesion and earlier timepoints should be assessed in the future.…”

Section: Discussionmentioning

confidence: 99%

The Bacterial Species Campylobacter jejuni Induce Diverse Innate Immune Responses in Human and Avian Intestinal Epithelial Cells

et al. 2017

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Campylobacter remain the major cause of human gastroenteritis in the Developed World causing a significant burden to health services. Campylobacter are pathogens in humans and chickens, although differences in mechanistic understanding are incomplete, in part because phenotypic strain diversity creates inconsistent findings. Here, we took Campylobacter jejuni isolates (n = 100) from multi-locus sequence typed collections to assess their pathogenic diversity, through their inflammatory, cytotoxicity, adhesion, invasion and signaling responses in a high-throughput model using avian and human intestinal epithelial cells. C. jejuni induced IL-8 and CXCLi1/2 in human and avian epithelial cells, respectively, in a MAP kinase-dependent manner. In contrast, IL-10 responses in both cell types were PI 3-kinase/Akt-dependent. C. jejuni strains showed diverse levels of invasion with high invasion dependent on MAP kinase signaling in both cell lines. C. jejuni induced diverse cytotoxic responses in both cell lines with cdt-positive isolates showing significantly higher toxicity. Blockade of endocytic pathways suggested that invasion by C. jejuni was clathrin- and dynamin-dependent but caveolae- independent in both cells. In contrast, IL-8 (and CXCLi1/2) production was dependent on clathrin, dynamin, and caveolae. This study is important because of its scale, and the data produced, suggesting that avian and human epithelial cells use similar innate immune pathways where the magnitude of the response is determined by the phenotypic diversity of the Campylobacter species.

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Section: Discussionmentioning

confidence: 99%

The Bacterial Species Campylobacter jejuni Induce Diverse Innate Immune Responses in Human and Avian Intestinal Epithelial Cells

et al. 2017

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“…histolytica infection. Although tissue slices have been well accepted for xenobiotic toxicity and metabolism studies 57,58 , as well as models for ex vivo infection with viruses 59,60 , bacteria 39 , and parasites 20,61 , the present work represents the first report of the in situ effect of compounds with anti-amoebic properties on the progression of infection by E . histolytica in organotypic culture of both, hepatic and intestinal tissues derived from the same animal.…”

Section: Discussionmentioning

confidence: 95%

Development of a Novel Ex-vivo 3D Model to Screen Amoebicidal Activity on Infected Tissue

Guzmán-Delgado

Carranza-Torres

García-Davis

et al. 2019

Sci Rep

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Amoebiasis is a parasitic disease that causes thousands of deaths every year, its adverse effects and resistance to conventional treatments have led to the search of new treatment options, as well as the development of novel screening methods. In this work, we implemented a 3D model of intestine and liver slices from hamsters that were infected ex vivo with virulent E . histolytica trophozoites. Results show preserved histology in both uninfected tissues as well as ulcerations, destruction of the epithelial cells, and inflammatory reaction in intestine slices and formation of micro abscesses, and the presence of amoebae in the sinusoidal spaces and in the interior of central veins in liver slices. The three chemically synthetized compounds T-001, T-011, and T-016, which act as amoebicides in vitro , were active in both infected tissues, as they decreased the number of trophozoites, and provoked death by disintegration of the amoeba, similar to metronidazole. However, compound T-011 induced signs of cytotoxicity to liver slices. Our results suggest that ex vivo cultures of precision-cut intestinal and liver slices represent a reliable 3D approach to evaluate novel amoebicidal compounds, and to simultaneously detect their toxicity, while reducing the number of experimental animals commonly required by other model systems.

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“…PCiS has also been used to evaluate drug metabolism and transport in mammalian systems [43][44][45] . in terms of the mucosal immune system, this chicken PCiS system can be used as an alternate avian infection model since it includes the intestinal lymphoid tissues for defense 46 . in the present study, both the chicken small intestine and colon were sliced to assess early events following gastrointestinal exposure to metals and Don under the assumption that these xenobiotics frequently contaminate the diet and drinking water.…”

Section: Discussionmentioning

confidence: 99%

In vitro estimation of metal-induced disturbance in chicken gut-oviduct chemokine circuit

Kim

Han

et al. 2019

Mol. Cell. Toxicol.

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Backgrounds:Heavy metals affect various processes in the embryonic development. embryonic fibroblasts (eFs) play key roles in the innate recognition and wound healing in reproductive tissues. Methods: Based on the relative toxicities of different inorganic metals and inorganic nonmetallic compounds against murine and chicken eF cells, mechanistic estimations were performed based on transcriptomic analyses. Results: lead (ii) acetate induced preferential injuries in the chicken eF and mechanistic analyses using transcriptome revealed that chemokine receptor-associated events are potently involved in metal-induced adverse actions. as an early sentinel of metal exposure, the precision-cut intestine slices (PCiS) induced the expression of chemokines including CXCli1 or CXCli2, which were potent gut-derived factors that activate chemokine receptors in reproductive organs after circulation. Conclusion: eF-selective metals can be estimated to trigger the chemokine circuit in the gut-reproductive axis of chickens. This in vitro methodology using PCiS-eF culture could be used as a promising alternate platform for the reproductive immunotoxicological assessment.

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Precision-cut intestinal slices as a culture system to analyze the infection of differentiated intestinal epithelial cells by avian influenza viruses

Cited by 11 publications

References 19 publications

The Bacterial Species Campylobacter jejuni Induce Diverse Innate Immune Responses in Human and Avian Intestinal Epithelial Cells

The Bacterial Species Campylobacter jejuni Induce Diverse Innate Immune Responses in Human and Avian Intestinal Epithelial Cells

Development of a Novel Ex-vivo 3D Model to Screen Amoebicidal Activity on Infected Tissue

In vitro estimation of metal-induced disturbance in chicken gut-oviduct chemokine circuit

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