Precision and long-term stability of capillary electrophoresis measurement of serum protein zones

Luraschi, Paola; Brambilla, Sônia Domingues Santos; Infusino, Ilenia; Franzini, Carlo

doi:10.1016/s0009-8981(03)00163-3

Cited by 10 publications

(5 citation statements)

References 16 publications

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“…They also found that CE had excellent within and between capillary precision of 0.6-3.3% (N = 10) and 1.8-4.5% (N = 120), respectively. The long-term reproducibility of Paragon 2000 has recently been confirmed in a study by Luraschi et al [41]. They found that the intercapillary and interinstrument precision over a 1 year period was less than 3.1% and a maximum QC imprecision of less than 6.1%.…”

Section: Serum Proteinsmentioning

confidence: 76%

“…1 and an overview of the technical details of the two instruments can be found in a review by Bossuyt [30]. Numerous papers have been written on the comparison of single capillary research [31][32][33][34][35][36][37][38][39] or commercial CZE instruments [40][41][42][43][44][45][46][47][48][49][50][51][52][53][54] with AGE or CAE. Jenkins et al [31][32][33] were the first to describe the comparison of CE with AGE using a large number of patient samples (N = 6500).…”

Section: Serum Proteinsmentioning

confidence: 99%

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Capillary electrophoresis and the clinical laboratory

et al. 2006

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Over the past 15 years, CE as an analytical tool has shown great promise in replacing many conventional clinical laboratory methods, such as electrophoresis and HPLC. CE's appeal was that it was fast, used very small amounts of sample and reagents, was extremely versatile, and was able to separate large and small analytes, whether neutral or charged. Because of this versatility, numerous methods have been developed for analytes that are of clinical interest. Other than molecular diagnostic and forensic laboratories CE has not been able to make a major impact in the United States. In contrast, in Europe and Japan an increasing number of clinical laboratories are using CE. Now that automated multicapillary instruments are commercially available along with cost-effective test kits, CE may yet be accepted as an instrument that will be routinely used in the clinical laboratories. This review will focus on areas where CE has the potential to have the greatest impact on the clinical laboratory. These include analyses of proteins found in serum and urine, hemoglobin (A1c and variants), carbohydrate-deficient transferrin, forensic and therapeutic drug screening, and molecular diagnostics.

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Section: Serum Proteinsmentioning

confidence: 76%

Section: Serum Proteinsmentioning

confidence: 99%

Capillary electrophoresis and the clinical laboratory

et al. 2006

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“…The instruments were fed the total protein values of the assayed sera, for automatic calculation of the zones' protein mass concentration, in g/L. Overall imprecision of CZE measurements of a-1-globulin, from longterm evaluation of system's stability (as monthly CV%) was in the interval 2.0%-8.4% (mean 5.2%) [15]. Immunonephelometric measurements of AAT, AAG and apolipoprotein A-I (ApoA) were carried out with an Immage kinetic immunonephelometer (Beckman Coulter).…”

Section: Methodsmentioning

confidence: 99%

High‐density lipoproteins contribute to the α‐1‐globulin zone in capillary electrophoresis of human serum proteins

et al. 2004

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Alpha-1-antitrypsin (AAT) and alpha-1-acid glycoprotein (AAG) are reported to be the main proteins contributing to the alpha-1-globulin capillary zone electrophoresis (CZE) zone, but the sum (AAT + AAG) showed lower than the alpha-1-globulin. We investigated the role of high-density lipoprotein (HDL), an additional protein migrating in the alpha-1-globulin zone, as a possible cause for such a gap. In a set of 98 sera we measured the alpha-1-globulin with a dedicated clinical capillary electrophoresis system, and AAT, AAG and apolipoprotein A-1 (ApoA) by immunonephelometry. The alpha-1-globulin were consistently higher than the sum (AAT + AAG), by (mean value +/- standard deviation) 1.70 +/- 0.88 g/L in 49 sera with low ApoA, and by 3.59 +/- 0.75 g/L in 49 sera with high ApoA. Corresponding figures in the comparison alpha-1-globulin/(AAT + AAG + ApoA) were reduced to 1.08 +/- 0.77 g/L and 1.67 +/- 0.70 g/L. It is concluded that HDL significantly contribute to the CZE alpha-1-globulin zone, but do not completely explain the differences between the electrophoretic and the immunochemical measurements. However, CZE alpha-1-globulin measurements give information about increases of the two major acute phase proteins comparable to specific protein measurements.

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“…Through the analysis of serum proteins, CZE could identify inflammation, nephrotic syndrome, bisalbuminemia, and a 1 -antitrypsin deficiency [193]. Quality control was performed extensively in CZE [194,195]. The method was found stable over 1-year period [195], and showed higher sensitivity (but lower specificity) than gel electrophoresis [193,196].…”

Section: Proteins In Bloodmentioning

confidence: 99%

“…Quality control was performed extensively in CZE [194,195]. The method was found stable over 1-year period [195], and showed higher sensitivity (but lower specificity) than gel electrophoresis [193,196]. Conflicting results between CZE and gel electrophoresis, and interferences of some substances including iodinated contrast agent and antibiotics, have also been observed [197].…”

Section: Proteins In Bloodmentioning

confidence: 99%

Capillary electrophoresis of proteins 2003–2005

Dolnı́k¹

2006

Electrophoresis

139

117

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This review article with 304 references describes recent developments in CE of proteins, and covers the two years since the previous review (Hutterer, K., Dolník, V., Electrophoresis 2003, 24, 3998-4012) through Spring 2005. It covers topics related to CE of proteins, including modeling of the electrophoretic migration of proteins, sample pretreatment, wall coatings, improving separation, various forms of detection, special electrophoretic techniques such as affinity CE, CIEF, and applications of CE to the analysis of proteins in real-world samples including human body fluids, food and agricultural samples, protein pharmaceuticals, and recombinant protein preparations.

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Precision and long-term stability of capillary electrophoresis measurement of serum protein zones

Cited by 10 publications

References 16 publications

Capillary electrophoresis and the clinical laboratory

Capillary electrophoresis and the clinical laboratory

High‐density lipoproteins contribute to the α‐1‐globulin zone in capillary electrophoresis of human serum proteins

Capillary electrophoresis of proteins 2003–2005

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