Precision and Accuracy of Dual‐Energy X‐ray Absorptiometry for Determining in Vivo Body Composition of Mice

Nagy, Tim R.; Clair, Anne-Laure

doi:10.1038/oby.2000.47

Cited by 261 publications

(235 citation statements)

References 13 publications

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“…The CVs obtained for FM and LTM using the Norland pDEXA instrument are reasonably good, but are nearly 2-fold higher than that shown for mice using the PIXImus densitometer (5). Similarly, CVs for TBBM and BMD in the present study are good, but are higher than found previously (5).…”

Section: Discussionsupporting

confidence: 63%

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Body Composition in a Seasonal Model of Obesity: Longitudinal Measures and Validation of DXA

Hunter

Nagy

2002

Obesity Research

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HUNTER, HEATHER L. AND TIM R. NAGY. Body composition in a seasonal model of obesity: longitudinal measures and validation of DXA. Obes Res. 2002;10: 1180 -1187. Objective: Collared lemmings, Dicrostonyx groenlandicus, show rapid changes in body mass on a seasonal basis. The objective of this study was to measure longitudinal changes in body composition in animals undergoing photoperiodinduced weight gain and loss using DXA. Research Methods and Procedures: Adult, female collared lemmings exposed to either long (LD; 22 hours light/2 hours dark) or short (SD; 8 hours light/16 hours dark) photoperiods were anesthetized, and DXA was used to determine fat mass, lean tissue mass (LTM), total-body bone mineral content, and total-bone mineral density. After a baseline scan, one-half of the animals were transferred to the alternate photoperiod (SD-LD, weight loss; LD-SD, weight gain) and one-half remained on the same photoperiod (controls; SD-SD, LD-LD). Body composition was determined by DXA after 4 and 8 weeks. Animals were killed, and body composition was determined by carcass analysis. DXA-derived data were validated by comparing with carcass analysis. Results: Body composition by DXA was highly related to body composition measured by chemical analysis, thereby justifying the use of DXA. Lemmings in the SD-LD group lost weight, and this was reflected in measurable losses of fat and LTM. Lemmings in the LD-SD group gained weight, which was shown by measurable increases in fat, LTM and total-body bone mineral content. Discussion: Comparison of body composition determined by DXA to that by chemical extraction revealed that DXA is useful for measuring body composition. The longitudinal analysis revealed that collared lemmings undergo rapid changes in body composition when exposed to changes in photoperiod.

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Section: Discussionsupporting

confidence: 63%

“…The collared lemming is one such model that shows large (5). In this study, we show that DXA is capable of measuring body composition in small rodents and use the technology to longitudinally measure photoperiod-induced changes in body composition in the collared lemming.…”

Section: Discussionmentioning

confidence: 75%

Body Composition in a Seasonal Model of Obesity: Longitudinal Measures and Validation of DXA

Hunter

Nagy

2002

Obesity Research

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“…The body weight, bone-free soft-lean tissue mass and fat mass in mice of 22 weeks of age were measured using DXA analysis technique as described previously [18]. For DXA analysis, the mice were sacrificed and kept at −80°C until they were analyzed.…”

Section: Animalsmentioning

confidence: 99%

Obesity increases the risk of UV radiation-induced oxidative stress and activation of MAPK and NF-κB signaling

Katiyar

Meeran

2007

Free Radical Biology and Medicine

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Obesity has been implicated in several diseases including cancer, however, the relationship of obesity and susceptibility to ultraviolet (UV) radiation-caused skin diseases has not been investigated. As UV-induced oxidative stress has been implicated in several skin diseases, we assessed the role of obesity on UVB-induced oxidative stress in genetically obese Lep ob /Lep ob (leptin-deficient) mice. Here, we report that chronic exposure to UVB (120 mJ/cm 2 ) resulted in greater oxidative stress in the skin of obese mice in terms of higher levels of H 2 O 2 and NO production, photo-oxidative damage of lipids and proteins, and greater depletion of antioxidant defense enzymes, like, glutathione, glutathione peroxidase and catalase. As UV-induced oxidative stress mediates activation of MAPK and NF-κB signaling pathways, we determined the effect of UVB on these pathways in obese mice. Exposure of obese mice to UVB resulted in phosphorylation of ERK1/2, JNK and p38 proteins of the MAPK family. Compared to wild-type mice, the obese mice exhibited higher levels of phosphorylation of these proteins, greater activation of NF-κB/p65, and higher levels of circulating proinflammatory cytokines, including TNF-α, IL-1-β and IL-6, on UVB irradiation. Together, our study suggests for the first time that obesity in mice is associated with greater susceptibility to UVBinduced oxidative stress, and therefore may be a risk factor for skin diseases associated with UVBinduced oxidative stress.

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“…25 Carcasses were thawed at room temperature and weighed to determine water loss due to freezing. Gastrointestinal tracts were removed and bodies were weighed to determine the eviscerated weight.…”

Section: Body Composition Analysismentioning

confidence: 99%

Antipsychotic drug-induced weight gain: development of an animal model

et al. 2005

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OBJECTIVE: Weight gain is a prominent effect of most atypical antipsychotic drugs (AAPDs); yet, the mechanisms are not fully understood and no well-established mouse models exist for investigating the mechanisms. Thus, we developed a mouse model to evaluate the effects of AAPDs on eating, body weight (BW), and body composition. METHODS: Female C57BL/6J mice were used to test olanzapine, quetiapine, ziprasidone, and risperidone. Mice were acclimated to individual housing, given ad libitum access to chow and water, dosed with placebo peanut butter pills for 1 week, and then dosed daily with AAPD-laced peanut butter pills for 4 weeks. Weekly food intakes and BWs were measured, and body compositions were determined at the end of each experiment. RESULTS: After 4 weeks of treatment, olanzapine, quetiapine, ziprasidone, and risperidone caused significant weight increases, but only olanzapine and quetiapine were associated with significantly increased food intake. Body composition data revealed that olanzapine-treated mice had more relative fat mass and risperidone-treated mice had more relative lean mass than did control mice. Quetiapine and ziprasidone did not significantly affect relative body composition even though BW was increased. CONCLUSIONS: Oral AAPD administration causes increased BW in female mice. Our mouse model of AAPD-induced weight gain resembles the human response to these medications and will be used to investigate the mechanisms for weight gain and fat accumulation.

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Precision and Accuracy of Dual‐Energy X‐ray Absorptiometry for Determining in Vivo Body Composition of Mice

Cited by 261 publications

References 13 publications

Body Composition in a Seasonal Model of Obesity: Longitudinal Measures and Validation of DXA

Body Composition in a Seasonal Model of Obesity: Longitudinal Measures and Validation of DXA

Obesity increases the risk of UV radiation-induced oxidative stress and activation of MAPK and NF-κB signaling

Antipsychotic drug-induced weight gain: development of an animal model

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