Precisely timed regulation of enhancer activity defines the binary expression pattern of Fushi tarazu in the Drosophila embryo

Birnie, Anthony; Plat, Audrey; Korkmaz, Cemil; Bothma, Jacques P.

doi:10.1016/j.cub.2023.04.005

Cited by 9 publications

(3 citation statements)

References 44 publications

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“…For each time point the average nuclear out-of-pattern and in-pattern GFP signal intensity was retrieved. An enrichment ratio was then calculated by dividing the in-pattern by the out-of-pattern GFP signal intensity in a time-dependent manner 82 .…”

Section: Methodsmentioning

confidence: 99%

Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organism

Pimmett,

Douaihy,

Maillard

et al. 2024

Preprint

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The ability to refine transcriptional levels via active repression in an euchromatic context represents a critical regulatory process during development. While the molecular players of active repression are well described, their dynamics remain largely obscure. By monitoring expression dynamics of the pro-EMT developmental gene snail in Drosophila embryos, we uncovered and quantified the timescale of kinetic bottlenecks tuning transcription during repression. Repression is associated with the transition of the promoter from two states to a three-state regime, comprising two temporally distinct inactive periods. Surprisingly, repression occurs without abrupt changes in Pol II initiation rates. By monitoring nuclear Sna protein levels, we show that Sna-mediated repression operates with high cooperativity, a feature dictating the degree of cell-cell coordination in the imposition of repression. Our approach offers quantitative insights into the dynamics of repression mediated by short-range repressors and how their cooperativity may coordinate cell fate decisions within a tissue.

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Section: Methodsmentioning

confidence: 99%

Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organism

Pimmett,

Douaihy,

Maillard

et al. 2024

Preprint

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show abstract

“…In addition, Zelda and Hunchback were found to help sharpen the boundary, where Zelda lowers the Bcd concentration threshold for transcription activation, and Hunchback reduces stochastic ON–OFF switching and increases the Pol II initiation rate [ 39 ]. Besides sensing TF concentration, gene expression during Drosophila development can be induced at defined times after a previous regulatory event, as shown for Fushi tarazu (Ftz) expression [ 40 ]. Multiple mechanisms thus allow the TF gradient to facilitate precise responses during development.…”

Section: The Molecular Drivers Of Transcriptional Burstingmentioning

confidence: 99%

Time will tell: comparing timescales to gain insight into transcriptional bursting

Meeussen,

Lenstra

2024

Trends in Genetics

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“…ftz mRNA is initially expressed as a continuous band before transitioning to a regular striped pattern ( Edgar et al, 1986 ). Without rapid turnover, the ability of Ftz protein to stimulate ftz transcription ( Birnie et al, 2023 ; Zhao et al, 2023 ) would prevent the restriction of the pattern to stripes. More generally, extensive degradation of maternal mRNA in animal embryos occurs to clear the slate for zygotic gene expression ( Schier, 2007 ).…”

Section: Rna Turnover To Destabilise Cell Statesmentioning

confidence: 99%

Clearing the slate: RNA turnover to enable cell state switching?

Westbrook,

Ford,

Antolović

et al. 2023

Development

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The distribution of mRNA in tissue is determined by the balance between transcription and decay. Understanding the control of RNA decay during development has been somewhat neglected compared with transcriptional control. Here, we explore the potential for mRNA decay to trigger rapid cell state transitions during development, comparing a bistable switch model of cell state conversion with experimental evidence from different developmental systems. We also consider another potential role for large-scale RNA decay that has emerged from studies of stress-induced cell state transitions, in which removal of mRNA unblocks the translation machinery to prioritise the synthesis of proteins that establish the new cell state.

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Precisely timed regulation of enhancer activity defines the binary expression pattern of Fushi tarazu in the Drosophila embryo

Cited by 9 publications

References 44 publications

Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organism

Dissecting the dynamics of coordinated active transcriptional repression in a multicellular organism

Time will tell: comparing timescales to gain insight into transcriptional bursting

Clearing the slate: RNA turnover to enable cell state switching?

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