Precisely Defined Conjugated Oligoelectrolytes for Biosensing and Therapeutics

Wang, Bing; Queenan, Bridget N.; Wang, Shu; Nilsson, K. Peter R.; Bazan, Guillermo C.

doi:10.1002/adma.201806701

Cited by 65 publications

(69 citation statements)

References 195 publications

(214 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These studies have demonstrated that effective and reversible photomodulation can be achieved both in vitro and in vivo, by the covalent binding of the azobenzene unit to the selected target. Alternatively, one can exploit non‐covalent interactions to assist the incorporation of photoactive molecules in bio‐systems 26–30. Although this method is less specific than the covalent approach, the relatively weak non‐covalent interactions still allow for driving affinity to various bio‐interfaces, while ensuring full reversibility and much lower invasiveness.…”

Section: Figurementioning

confidence: 99%

Membrane Environment Enables Ultrafast Isomerization of Amphiphilic Azobenzene

et al. 2020

View full text Add to dashboard Cite

The non‐covalent affinity of photoresponsive molecules to biotargets represents an attractive tool for achieving effective cell photo‐stimulation. Here, an amphiphilic azobenzene that preferentially dwells within the plasma membrane is studied. In particular, its isomerization dynamics in different media is investigated. It is found that in molecular aggregates formed in water, the isomerization reaction is hindered, while radiative deactivation is favored. However, once protected by a lipid shell, the photochromic molecule reacquires its ultrafast photoisomerization capacity. This behavior is explained considering collective excited states that may form in aggregates, locking the conformational dynamics and redistributing the oscillator strength. By applying the pump probe technique in different media, an isomerization time in the order of 10 ps is identified and the deactivation in the aggregate in water is also characterized. Finally, it is demonstrated that the reversible modulation of membrane potential of HEK293 cells via illumination with visible light can be indeed related to the recovered trans→cis photoreaction in lipid membrane. These data fully account for the recently reported experiments in neurons, showing that the amphiphilic azobenzenes, once partitioned in the cell membrane, are effective light actuators for the modification of the electrical state of the membrane.

show abstract

Section: Figurementioning

confidence: 99%

Membrane Environment Enables Ultrafast Isomerization of Amphiphilic Azobenzene

et al. 2020

View full text Add to dashboard Cite

show abstract

“…These conformational‐dependent optical characteristics make LCOs promising biorecognition element for the selective and sensitive detection of biomacromolecules. [ 152 ]…”

Section: Optotracing By Luminescent‐conjugated Oligothiophenesmentioning

confidence: 99%

Bacterial Sensing and Biofilm Monitoring for Infection Diagnostics

Parlak

Richter‐Dahlfors

2020

Macromolecular Bioscience

View full text Add to dashboard Cite

Recent insights into the rapidly emerging field of bacterial sensing and biofilm monitoring for infection diagnostics are discussed as well as recent key developments and emerging technologies in the field. Electrochemical sensing of bacteria and bacterial biofilm via synthetic, natural, and engineered recognition, as well as direct redox-sensing approaches via algorithmbased optical sensing, and tailor-made optotracing technology are discussed. These technologies are highlighted to answer the very critical question: "how can fast and accurate bacterial sensing and biofilm monitoring be achieved? Following on from that: "how can these different sensing concepts be translated for use in infection diagnostics? A central obstacle to this transformation is the absence of direct and fast analysis methods that provide high-throughput results and bio-interfaces that can control and regulate the means of communication between biological and electronic systems. Here, the overall progress made to date in building such translational efforts at the level of an individual bacterial cell to a bacterial community is discussed.

show abstract

“…Recently, cationic conjugated oligomers (including cationic oligo(p-phenylene vinylene), oligo(p-phenylene ethynylene)s, and oligo-(thiophene ethynylene) (OTE)), exhibit high antibacterial and antitumor activity on account of their noticeable PDT effect. [30][31][32] In addition, cationic conjugated oligomers are usually featured with strong light-harvesting ability and high fluorescence quantum yield, 33,34 which forms the solid base for investigating the dynamic distribution of drugs inside cells. 35 Particularly, OTE can physically break the membrane structure of microorganism efficiently.…”

Section: Introductionmentioning

confidence: 99%