Precisely Controlled Three-Dimensional Gold Nanoparticle Assembly Based on Spherical Bacteriophage Scaffold for Molecular Sensing via Surface-Enhanced Raman Scattering

Jeon, Myeong Jin; Ma, Xianyong; Lee, Jong Uk; Roh, Hyejin; Bagot, Conrad Corbella; Park, Won; Sim, Sang Jun

doi:10.1021/acs.jpcc.0c08800

Cited by 10 publications

(6 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The analyses of TEM and energy‐dispersive X‐ray (EDX) spectroscopy confirm that the dark blue color comes from large Au particles, that is, the larger the size of Au particles is, the darker the blue color becomes (Figure S1e,f ). Interestingly, light absorption spectra shows that as the color gradually darkens the maximum absorbance always occurs at a wavelength of 620 nm ( λ max ) (Figure 3a,b ), which is in good agreement with the “raspberry” model 56 , 57 that was established to explain optical properties of gold particles (i.e., clusters of AuNPs). According to this model, larger clusters of AuNPs possess a larger absorption cross section per cluster, while resonance wavelength remained nearly constant.…”

Section: Resultssupporting

confidence: 83%

A simple urine test by 3D‐plus‐3D immunoassay guides precise in vitro cancer diagnosis

Kim

Moon

Seol

et al. 2023

Bioengineering & Transla Med

View full text Add to dashboard Cite

Although a variety of urinary cancer markers are available for in vitro diagnosis, inherent problems of urine environment—containing various inorganic/organic ions/molecules that vary in concentration over a 20‐fold range or more and significantly attenuate antibody avidity for markers—render conventional immunoassays unsuitable, remaining unresolved and a major challenge. Here we developed a 3D‐plus‐3D (3p3) immunoassay method, based on a single‐step urinary marker detection by 3D‐antibody probes, which are free of steric hindrance and capable of omnidirectional capture of markers in a 3D solution. The 3p3 immunoassay showed an excellent performance in the diagnosis of prostate cancer (PCa) through detecting PCa‐specific urinary engrailed‐2 protein, demonstrating 100% sensitivity and 100% specificity with the urine specimens of PCa‐related and other related disease patients and healthy individuals. This innovative approach holds a great potential in opening up a novel clinical route for precise in vitro cancer diagnosis and also pushing urine immunoassay closer to more widespread adoption.

show abstract

Section: Resultssupporting

confidence: 83%

A simple urine test by 3D‐plus‐3D immunoassay guides precise in vitro cancer diagnosis

Kim

Moon

Seol

et al. 2023

Bioengineering & Transla Med

View full text Add to dashboard Cite

show abstract

“…In comparison to other noble metal-based NPs, the assembled structure had a lower EF value owing to the large surface area for RLC binding. However, the higher intensity and signal uniformity of each single nanocomposite could be advantageous features of assembled structures [44]. SiO 2 @Au@Au has higher EF values than those reported for other noble metal-assembled NPs (Table 1).…”

Section: Resultsmentioning

confidence: 85%

Highly Sensitive Near-Infrared SERS Nanoprobes for in Vivo Imaging Using Gold-Assembled Silica Nanoparticles with Controllable Nanogaps

Bock

Choi

Kim

et al. 2021

Preprint

View full text Add to dashboard Cite

Backgroud: Surface-enhanced Raman scattering (SERS) imaging is widely exploited, given its advantages such as multiplex capacity, non-photobleaching property, and high sensitivity. Near-infrared (NIR) radiation is suitable for in vivo studies because it exhibits good tissue penetration capability. Results In this study, gold (Au)-assembled silica (SiO2) nanoparticles (SiO2@Au@Au NPs) as NIR SERS nanoprobes are synthesized by a seed-mediated growth method. SiO2@Au@Au NPs with six different sizes of Au NPs are prepared by controlling the concentration of the Au precursor in the growth step. Therefore, the surface plasmonic band of the nanogaps between Au NPs on the SiO2 surface could be controlled from 4.16 to 0.98 nm, thus generating SERS hotspots. SiO2@Au@Au NPs with a 0.98-nm gap shows high SERS signals after being subjected to an excitation wavelength of 785 nm (enhancement factor ~3.8 × 106). SiO2@Au@Au nanoprobes shows detectable in vivo SERS signals at a concentration of 16 µg/mL in a 7-mm-thick animal tissue specimen. SiO2@Au@Au NPs with 14 different Raman label compounds shows distinguishable SERS signals upon being subcutaneously injected into nude mice. Conclusion Through this study, it is highlighted that their potential for use in in vivo applications as multiplex nanoprobes.

show abstract

“…Compared to other noble metal-based NPs, the assembled structure had a lower EF value owing to the large surface area for RLC binding. However, the higher intensity and signal uniformity of each nanocomposite could be an advantageous feature of the assembled structures [ 44 ]. SiO 2 @Au@Au 500 exhibited higher EF values than those reported for other noble metal-assembled NPs (Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

Highly sensitive near-infrared SERS nanoprobes for in vivo imaging using gold-assembled silica nanoparticles with controllable nanogaps

et al. 2022

View full text Add to dashboard Cite

Background To take advantages, such as multiplex capacity, non-photobleaching property, and high sensitivity, of surface-enhanced Raman scattering (SERS)-based in vivo imaging, development of highly enhanced SERS nanoprobes in near-infrared (NIR) region is needed. A well-controlled morphology and biocompatibility are essential features of NIR SERS nanoprobes. Gold (Au)-assembled nanostructures with controllable nanogaps with highly enhanced SERS signals within multiple hotspots could be a breakthrough. Results Au-assembled silica (SiO2) nanoparticles (NPs) (SiO2@Au@Au NPs) as NIR SERS nanoprobes are synthesized using the seed-mediated growth method. SiO2@Au@Au NPs using six different sizes of Au NPs (SiO2@Au@Au50–SiO2@Au@Au500) were prepared by controlling the concentration of Au precursor in the growth step. The nanogaps between Au NPs on the SiO2 surface could be controlled from 4.16 to 0.98 nm by adjusting the concentration of Au precursor (hence increasing Au NP sizes), which resulted in the formation of effective SERS hotspots. SiO2@Au@Au500 NPs with a 0.98-nm gap showed a high SERS enhancement factor of approximately 3.8 × 106 under 785-nm photoexcitation. SiO2@Au@Au500 nanoprobes showed detectable in vivo SERS signals at a concentration of 16 μg/mL in animal tissue specimen at a depth of 7 mm. SiO2@Au@Au500 NPs with 14 different Raman label compounds exhibited distinct SERS signals upon subcutaneous injection into nude mice. Conclusions SiO2@Au@Au NPs showed high potential for in vivo applications as multiplex nanoprobes with high SERS sensitivity in the NIR region. Graphical Abstract

show abstract

Precisely Controlled Three-Dimensional Gold Nanoparticle Assembly Based on Spherical Bacteriophage Scaffold for Molecular Sensing via Surface-Enhanced Raman Scattering

Cited by 10 publications

References 63 publications

A simple urine test by 3D‐plus‐3D immunoassay guides precise in vitro cancer diagnosis

A simple urine test by 3D‐plus‐3D immunoassay guides precise in vitro cancer diagnosis

Highly Sensitive Near-Infrared SERS Nanoprobes for in Vivo Imaging Using Gold-Assembled Silica Nanoparticles with Controllable Nanogaps

Highly sensitive near-infrared SERS nanoprobes for in vivo imaging using gold-assembled silica nanoparticles with controllable nanogaps

Contact Info

Product

Resources

About