Precise Synergistic Photothermal Therapy Guided by Accurate Temperature‐Dependent NIR‐II Fluorescence Imaging
Zhuqing Sun,
Tuanwei Li,
Feng Wu
et al.
Abstract:Photothermal therapy (PTT) is considered a promising treatment strategy for solid tumors. However, local hyperthermia (over 45°C) during PTT can cause severe side effects in neighboring healthy tissues. PTT with accurate temperature feedback is a compelling strategy to ablate tumors and reduce side effects, but it still faces challenges. Here, a new kind of phototheranostic nanoparticle, namely 17‐RF@Ag2Se is developed, enabling in vivo NIR‐II fluorescence tracking, PTT and fluorescence nanothermometry as well… Show more
“…The mass of LSMPC-0.5 did not change within 7 days ( Supplementary Figure 1 ), indicating the non-degradability of LSMPC-0.5. We have reported that CSC degraded by 12% after 8 weeks, 36 which would provide space for new bone growth and osseointegration. Narrow facet interbody fusion devices currently used in minimally invasive interbody fusion are prone to interbody stress shielding, leading to vertebral collapse and fusion failure.…”
Section: Resultsmentioning
confidence: 99%
“…Previous research reported that it can cause damage to tissues when the temperature is over 45 °C for a long period. 36 Although the transformation temperature of the prepared LSMPC is about 45 °C, the shape recovery can be completed within 5 minutes, indicating limited thermal damage to human tissues. Figure 7 Feasibility of spinal fusion device.…”
Introduction
Lumbar interbody fusion is widely employed for both acute and chronic spinal diseases interventions. However, large incision created during interbody cage implantation may adversely impair spinal tissue and influence postoperative recovery. The aim of this study was to design a shape memory interbody fusion device suitable for small incision implantation.
Methods
In this study, we designed and fabricated an intervertebral fusion cage that utilizes near-infrared (NIR) light-responsive shape memory characteristics. This cage was composed of bisphenol A diglycidyl ether, polyether amine D-230, decylamine and iron oxide nanoparticles. A self-hardening calcium phosphate-starch cement (CSC) was injected internally through the injection channel of the cage for healing outcome improvement.
Results
The size of the interbody cage is reduced from 22 mm to 8.8 mm to minimize the incision size. Subsequent NIR light irradiation prompted a swift recovery of the cage shape within 5 min at the lesion site. The biocompatibility of the shape memory composite was validated through in vitro MC3T3-E1 cell (osteoblast-like cells) adhesion and proliferation assays and subcutaneous implantation experiments in rats. CSC was injected into the cage, and the relevant results revealed that CSC is uniformly dispersed within the internal space, along with the cage compressive strength increasing from 12 to 20 MPa.
Conclusion
The results from this study thus demonstrated that this integrated approach of using a minimally invasive NIR shape memory spinal fusion cage with CSC has potential for lumbar interbody fusion.
“…The mass of LSMPC-0.5 did not change within 7 days ( Supplementary Figure 1 ), indicating the non-degradability of LSMPC-0.5. We have reported that CSC degraded by 12% after 8 weeks, 36 which would provide space for new bone growth and osseointegration. Narrow facet interbody fusion devices currently used in minimally invasive interbody fusion are prone to interbody stress shielding, leading to vertebral collapse and fusion failure.…”
Section: Resultsmentioning
confidence: 99%
“…Previous research reported that it can cause damage to tissues when the temperature is over 45 °C for a long period. 36 Although the transformation temperature of the prepared LSMPC is about 45 °C, the shape recovery can be completed within 5 minutes, indicating limited thermal damage to human tissues. Figure 7 Feasibility of spinal fusion device.…”
Introduction
Lumbar interbody fusion is widely employed for both acute and chronic spinal diseases interventions. However, large incision created during interbody cage implantation may adversely impair spinal tissue and influence postoperative recovery. The aim of this study was to design a shape memory interbody fusion device suitable for small incision implantation.
Methods
In this study, we designed and fabricated an intervertebral fusion cage that utilizes near-infrared (NIR) light-responsive shape memory characteristics. This cage was composed of bisphenol A diglycidyl ether, polyether amine D-230, decylamine and iron oxide nanoparticles. A self-hardening calcium phosphate-starch cement (CSC) was injected internally through the injection channel of the cage for healing outcome improvement.
Results
The size of the interbody cage is reduced from 22 mm to 8.8 mm to minimize the incision size. Subsequent NIR light irradiation prompted a swift recovery of the cage shape within 5 min at the lesion site. The biocompatibility of the shape memory composite was validated through in vitro MC3T3-E1 cell (osteoblast-like cells) adhesion and proliferation assays and subcutaneous implantation experiments in rats. CSC was injected into the cage, and the relevant results revealed that CSC is uniformly dispersed within the internal space, along with the cage compressive strength increasing from 12 to 20 MPa.
Conclusion
The results from this study thus demonstrated that this integrated approach of using a minimally invasive NIR shape memory spinal fusion cage with CSC has potential for lumbar interbody fusion.
Exploiting photothermal agents with second‐near‐infrared (NIR‐II, 1000−1700 nm) emission and a high photothermal conversion efficiency (PCE) is an appealing and challenging task. Herein, by simultaneously tailoring the D−A interaction and planarity in fluorophores, two donor‐acceptor (D−A) −type semiconducting polymers (SPs), T‐BTP and B‐BTP, are constructed. Compared with T‐BTP, B‐BTP shows increased intramolecular interactions and improved molecular planarity, leading to bathochromic‐shift absorption, NIR‐II emission, and high PCE. Notably, the B‐BTP NPs achieve a remarkable PCE of 84.7%, which is among the highest PCEs of SPs in NIR‐II fluorescence imaging‐guided photothermal therapy (PTT). Because of these promising features, B‐BTP NPs are successfully used in NIR‐II vascular imaging and cancer therapy. This study provides valuable guidelines for the development of high‐performance NIR‐II SPs.
The lack of a simple design strategy to obtain ideal conjugated polymers (CPs) with high absorbance and fluorescence (FL) in the near-infrared-II (NIR-II; 1000–1700 nm) region still hampers the success of NIR-II light-triggered phototheranostics. Herein, novel phototheranostic nanoparticles (PPN-NO NPs) were successfully prepared by coloading a cationic NIR-II CPs (PBC-co-PBF-NMe
3
) and a NO donor (
S
-nitroso-
N
-acetylpenicillamine, SNAP) onto a 1:1 mixture of DSPE-PEG
5000
and dimyristoylphosphatidylcholine (DMPC) for NIR-II FL and NIR-II photoacoustic (PA) imaging-guided low-temperature NIR-II photothermal therapy (PTT) and gas combination therapy for cancer treatment. A precise NIR-II FL dually enhanced design tactic was proposed herein by integrating flexible nonconjugated segments (C6) into the CPs backbone and incorporating quaternary ammonium salt cationic units into the CPs side chain, which considerably increased the radiative decay pathway, resulting in desirable NIR-II FL intensity and balanced NIR-II absorption and NIR PTT properties. The phototheranostic PPN-NO NPs exhibited distinguished NIR-II FL and PA imaging performance in tumor-bearing mice models. Furthermore, the low-temperature photothermal effect of PPN-NO NPs could initiate NO release upon 980 nm laser irradiation, efficiently suppressing tumor growth owing to the combination of low-temperature NIR-II PTT and NO gas therapy in vitro and in vivo.
Graphical Abstract
Supplementary Information
The online version contains supplementary material available at 10.1186/s12951-024-02741-9.
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