Precise Spatiotemporal Identification of Mitochondrial H₂S Fluctuations through Exploiting an On-Demand Photoactivated Probe

Engineering organelle-specific activatable molecules for ultra-fast and reliable in situ mapping of subcellular nitric oxide

“…44–47 BODIPY fluorescent dyes have the advantages of good photostability, insensitivity to pH, and high fluorescence quantum yields. 48–52 We expected to construct a generalized platform that could reduce the reaction time of the probe and improve its detection sensitivity, enabling in situ and high-fidelity imaging of NO within various organelles. As shown in Schemes 1 and 2, taking the ER and lysosome as examples, in order to enable subcellular targeting of probes, we constructed ER-targeting BODIPY luminogens (ER-BOD) and lysosome-targeting BODIPY luminogens (Lyso-BOD), respectively.…”

Silica Nanoparticle-Based FRET System for Hydrogen Sulfide Detection in Biological and Food Samples

“…In the realm of H 2 S detection, significant challenges hinder the accuracy and efficacy of current methods, particularly due to the low H 2 S concentrations in early stage diseases and food spoilage. − Further complicating the detection process is the inherent complexity of liquid samples, such as blood or environmental water, which are often replete with a diverse array of compounds including gases, ions, and organic compounds. , These components can potentially interfere with H 2 S detection, thus presenting a formidable obstacle to precise quantification. − …”

High-Fidelity Spatiotemporal Recognition of Golgi ALP through an Initial-Accumulation and Postactivation Strategy

“…Fortunately, the photocontrolled strategy provides temporal and spatial controls over the response reactivity of fluorescence probes, affording a promising opportunity to exclude the above defect. − Herein, based on the continuous light and enzyme-activation tactic, we designed and synthesized the first photocontrollable and Golgi-targeted fluorescence probe BOD-P-Gol. Our designed molecules are described in Scheme , wherein the phenylsulfonamide unit is the targeting group of the Golgi, a boron-dipyrromethene (BODIPY) core is the fluorescence reporter, and a phosphate group unit is the ALP reaction trigger, which is locked with two photolabile 2-nitrobenzyl groups to prevent its response to ALP.…”