Precise Organization of Metal and Metal Oxide Nanoclusters into Arbitrary Patterns on DNA Origami

Biological materials are self-assembled with near-atomic precision in living cells, whereas synthetic 3D structures generally lack such precision and controllability. Recently, DNA nanotechnology, especially DNA origami technology, has been useful in the bottom-up fabrication of well-defined nanostructures ranging from tens of nanometres to sub-micrometres. In this Primer, we summarize the methodologies of DNA origami technology, including origami design, synthesis, functionalization and characterization. We highlight applications of origami structures in nanofabrication, nanophotonics and nanoelectronics, catalysis, computation, molecular machines, bioimaging, drug delivery and biophysics. We identify challenges for the field, including size limits, stability issues and the scale of production, and discuss their possible solutions. We further provide an outlook on next-generation DNA origami techniques that will allow in vivo synthesis and multiscale manufacturing.

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“…• Enzyme-driven DNA origami rotors 223 • Single-molecule DNA navigator 43 • Free-style metallization 131,308…”

mentioning

confidence: 99%

DNA origami

Dey

Chen

Gothelf

et al. 2021

Nat Rev Methods Primers

485

386

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“…The greatest metric of DNA origami technology is to offer multiple sites to position molecules, biomolecules and materials with a nanometer resolution. A wide range of nanomaterials can be incorporated in DNA origami nanostructure including polymers [107], nanodimonds [108], enzymes [109], metal NPs [110], quantum dots [111] and so on. However, the function derived from integration has little actual practical application including sensing.…”

Section: Discussionmentioning

confidence: 99%

DNA Origami-Enabled Biosensors

Wang

Zhou

et al. 2020

Sensors

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Biosensors are small but smart devices responding to the external stimulus, widely used in many fields including clinical diagnosis, healthcare and environment monitoring, etc. Moreover, there is still a pressing need to fabricate sensitive, stable, reliable sensors at present. DNA origami technology is able to not only construct arbitrary shapes in two/three dimension but also control the arrangement of molecules with different functionalities precisely. The functionalization of DNA origami nanostructure endows the sensing system potential of filling in weak spots in traditional DNA-based biosensor. Herein, we mainly review the construction and sensing mechanisms of sensing platforms based on DNA origami nanostructure according to different signal output strategies. It will offer guidance for the application of DNA origami structures functionalized by other materials. We also point out some promising directions for improving performance of biosensors.

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“…In a recent example, controlled placement of thiol groups on a DNA origami triangle was exploited to direct the growth of a variety of metal and metal oxide shapes that would be very difficult to grow through crystal growth techniques. 129 A follow-up study described the successful growth of silica nanostruc-tures using the same approach, further broadening the scope. 130 DNA was also used to controllably place mineralising groups to achieve controlled growth of calcium phosphate.…”

Section: [H2] Templated Assemblymentioning

confidence: 99%

Synthesis and applications of anisotropic nanoparticles with precisely defined dimensions

et al. 2020

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Precise Organization of Metal and Metal Oxide Nanoclusters into Arbitrary Patterns on DNA Origami

Abstract: Experimental section, extra figures, and results (PDF)

Cited by 68 publications

References 43 publications

DNA origami

DNA origami

DNA Origami-Enabled Biosensors

Synthesis and applications of anisotropic nanoparticles with precisely defined dimensions

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