Precise measurement of molecular phenotypes with barcode-based CRISPRi systems
Joseph H. Lobel,
Nicholas T. Ingolia
Abstract:SummaryGenome-wide CRISPR-Cas9 screens have untangled regulatory networks and revealed the genetic underpinnings of diverse biological processes. Their success relies on experimental designs that interrogate specific molecular phenotypes and distinguish key regulators from background effects. Here, we realize these goals with a generalizable platform for CRISPR interference with barcoded expression reporter sequencing (CiBER-seq) that dramatically improves the sensitivity and scope of genome-wide screens. We s… Show more
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