1979
DOI: 10.1093/nar/6.1.41
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Precise location of DNase I cutting sites in the nucleosome core determined by high resolution gel electrophoresis

Abstract: The precise locations of the DNase I cutting sites in the nucleosome core have been determined by analysis of the DNA products of a DNase I digestion of 32p end-labelled nucleosome cores on a high resolution gel electrophoresis system. This system is capable of resolving fragments of mixed sequence DNA differing by one base into the region of 160 bases in length. The DNase I cutting sites in the core are found to be spaced at multiples of about 10.4 (i.e. clearly different from 10.0) bases along the DNA, but s… Show more

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Cited by 209 publications
(150 citation statements)
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References 23 publications
(63 reference statements)
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“…and S12W, or Slc and S13w, with staggers varying from about 1.4 to about 3.0 bases (8). This variability of staggers means, as discussed earlier (8),…”
Section: Analysis Of Dnase I Cutting Results In Three Dimensionssupporting
confidence: 60%
See 3 more Smart Citations
“…and S12W, or Slc and S13w, with staggers varying from about 1.4 to about 3.0 bases (8). This variability of staggers means, as discussed earlier (8),…”
Section: Analysis Of Dnase I Cutting Results In Three Dimensionssupporting
confidence: 60%
“…The locations of DNAse I cleavage sites along one strand of the nucleosomal DNA have been determined using high resolution gel electrophoresis to measure the lengths of the single-stranded DNA fragments produced by the action of DNAse I on 5'-terminal radioactively labelled nucleosome cores (8).…”
Section: Analysis Of Dnase I Cutting Results In Three Dimensionsmentioning
confidence: 99%
See 2 more Smart Citations
“…Sequencing gels were prepared according to standard protocols, except that the acrylamide/bisacrylamide weight ratio was decreased from the usual 19:1 value to 12:1, to better resolve mixed-sequence fragments. (We failed to decrease that ratio to 6 -8:1, as described, 23,24 because the gel would not stick onto the glass plates. )…”
Section: Dnase I Footprintingmentioning
confidence: 99%