Precise identification of two wheat–Thinopyrum intermedium substitutions reveals the compensation and rearrangement between wheat and Thinopyrum chromosomes

Guang-rong, LI; Lang, Tao; Dai, Gang Shu; Li, Donghai; Li, Chenghui; Song, Xiao-Jin; Yang, Zujun

doi:10.1007/s11032-015-0202-z

Cited by 149 publications

(33 citation statements)

References 30 publications

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“…cristatum 6P chromosomes. Similar phenomena were also reported in other species, such as Leymus racemosus [42], Thinopyrum intermedium [43], and rye [44]. Therefore, 5113 was a novel wheat- A .…”

Section: Discussionsupporting

confidence: 79%

Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113

Pan

et al. 2016

PLoS ONE

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Creation of wheat-alien disomic addition lines and localization of desirable genes on alien chromosomes are important for utilization of these genes in genetic improvement of common wheat. In this study, wheat-Agropyron cristatum derivative line 5113 was characterized by genomic in situ hybridization (GISH) and specific-locus amplified fragment sequencing (SLAF-seq), and was demonstrated to be a novel wheat-A. cristatum disomic 6P addition line. Compared with its parent Fukuhokomugi (Fukuho), 5113 displayed multiple elite agronomic traits, including higher uppermost internode/plant height ratio, larger flag leaf, longer spike length, elevated grain number per spike and spikelet number per spike, more kernel number in the middle spikelet, more fertile tiller number per plant, and enhanced resistance to powdery mildew and leaf rust. Genes conferring these elite traits were localized on the A. cristatum 6P chromosome by using SLAF-seq markers and biparental populations (F1, BC1F1 and BC1F2 populations) produced from the crosses between Fukuho and 5113. Taken together, chromosomal localization of these desirable genes will facilitate transferring of high-yield and high-resistance genes from A. cristatum into common wheat, and serve as the foundation for the utilization of 5113 in wheat breeding.

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Section: Discussionsupporting

confidence: 79%

Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113

Pan

et al. 2016

PLoS ONE

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“…In this study, we successfully transferred Th. intermedium chromosome 1St#2 to wheat and subsequently produced substitution and translocation lines (Hu et al 2011;Li et al 2013Li et al , 2015a which enabled us to determine the effect of 1St#2 chromosomes in a wheat background. Our results suggest that the Th.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the Th. intermedium 1St#2 chromosome has also been found to carry novel stripe rust resistance genes, which are easily transferred into wheat (Hu et al 2011;Li et al 2015a). Hence, group-1 chromosomes derived from different Th.…”

Section: Discussionmentioning

confidence: 99%

“…Translocation lines E9-2 (2n = 42, T1DL.1St#2S) and E1233 (2n = 42, T1DS.1St#2L) were developed from crosses between AS1677 with the wheat cultivar MY11 . Line X479 is a double 1St#2 (1B) plus 4St/4J S (4B) substitution, and Line X482 is a double 4St/4J S (4D) plus 6St (6D) substitution line; both were selected from the progenies of the cross between TE-3 and wheat cultivar MY26 (Li et al 2015a). Pseudoroegneria spicata PI 232131 (St genome, 2n = 2x = 14) and Th.…”

Section: Plant Materialsmentioning

confidence: 99%

“…intermedium ssp. trichophorum chromosome 1St#2 substitution and translocation lines, which contained novel agronomically important genes (Hu et al 2011;Li et al 2013Li et al , 2015a. With the aim to precisely trace the 1St#2 chromosomes by marker assisted selection in wheat breeding programs for improving grain quality, the SLAF-seq technology was applied to wheat-Th.…”

Section: Introductionmentioning

confidence: 99%

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New molecular markers and cytogenetic probes enable chromosome identification of wheat-Thinopyrum intermedium introgression lines for improving protein and gluten contents

Guang-rong

Wang

Lang

et al. 2016

Planta

Self Cite

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New molecular markers were developed for targeting Thinopyrum intermedium 1St#2 chromosome, and novel FISH probe representing the terminal repeats was produced for identification of Thinopyrum chromosomes. Thinopyrum intermedium has been used as a valuable resource for improving the disease resistance and yield potential of wheat. A wheat-Th. intermedium ssp. trichophorum chromosome 1St#2 substitution and translocation has displayed superior grain protein and wet gluten content. With the aim to develop a number of chromosome 1St#2 specific molecular and cytogenetic markers, a high throughput, low-cost specific-locus amplified fragment sequencing (SLAF-seq) technology was used to compare the sequences between a wheat-Thinopyrum 1St#2 (1D) substitution and the related species Pseudoroegneria spicata (St genome, 2n = 14). A total of 5142 polymorphic fragments were analyzed and 359 different SLAF markers for 1St#2 were predicted. Thirty-seven specific molecular markers were validated by PCR from 50 randomly selected SLAFs. Meanwhile, the distribution of transposable elements (TEs) at the family level between wheat and St genomes was compared using the SLAFs. A new oligo-nucleotide probe named Oligo-pSt122 from high SLAF reads was produced for fluorescence in situ hybridization (FISH), and was observed to hybridize to the terminal region of 1St#L and also onto the terminal heterochromatic region of Th. intermedium genomes. The genome-wide markers and repetitive based probe Oligo-pSt122 will be valuable for identifying Thinopyrum chromosome segments in wheat backgrounds.

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Advances in Molecular Techniques of Insect Resistance in Cereal Improvement

Aarthi

Selvanarayanan²,

Tanda³

2022

Molecular Advances in Insect Resistance of Field Crops

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Precise identification of two wheat–Thinopyrum intermedium substitutions reveals the compensation and rearrangement between wheat and Thinopyrum chromosomes

Cited by 149 publications

References 30 publications

Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113

Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113

New molecular markers and cytogenetic probes enable chromosome identification of wheat-Thinopyrum intermedium introgression lines for improving protein and gluten contents

Advances in Molecular Techniques of Insect Resistance in Cereal Improvement

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