Precise diagnosis of
            <i>Neisseria macacae</i>
            infective endocarditis assisted by nanopore sequencing

Ai, Jingwen; Liu, Hong; Li, Huixia; Ling, Qingxia; Ai, Yan-Qin; Sun, Sijia; Wang, Xuan; Zhang, Bingyan; Zheng, Jianming; Jin, Jialin; Zhang, Wenhong

doi:10.1080/22221751.2020.1807411

Cited by 4 publications

(3 citation statements)

References 12 publications

(17 reference statements)

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“…Nanopore sequencing is another sequencing method, which is not used in the current study. However, we performed the first explorative study with nanopore sequencing in infectious endocarditis previously (Ai et al, 2020). In addition, we developed a rapid CRISPR-based assay for tuberculosis detection in various forms of direct clinical samples, which is also one of the pathogens causing pneumonia in MG patients (Ai et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Pneumonia in myasthenia gravis: Microbial etiology and clinical management

Jin

Jiao

et al. 2022

Front. Cell. Infect. Microbiol.

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IntroductionPatients with myasthenia gravis (MG) are prone to the development of pneumonia due to the long-term immunotherapies they receive and a tendency for aspiration. Pneumonia remains a risk factor for MG worsening and is the most prevalent cause of mortality in MG patients. Classification of the pathogens involved and exploration of the risk factors for mechanical ventilation (MV) could aid in improving clinical outcomes.MethodsBetween January 2013 and October 2022, we performed an inpatient database review for MG patients with pneumonia concurrence in a tertiary research center specializing in neuromuscular disorders. The clinical and microbiological characteristics of 116 MG patients with pneumonia were retrospectively analyzed.ResultsIn our cohort, 90.32% (112/124) of organisms were bacteria and 42.86% (48/112) of pathogenic bacteria were carbapenem-resistant. A high abundance of Epstein–Barr virus (EBV) was detected using next-generation sequencing (NGS) in 12 patients, while cytomegalovirus (CMV) was detected in 8 patients. Non-fermentative Gram-negative bacilli were the most prevalent microorganisms, in which ampicillin, sulfamethoxazole-trimethoprim (SMZ-TMP), piperacillin, cefoperazone, ceftazidime, and cefepime may have an anti-infectious effect. Moreover, peripheral lymphocyte percentage [odds ratio (OR) 0.88, 95% CI 0.75–0.96, p = 0.02] and serum globulin (OR 1.16, 95% CI 1.02–1.35, p = 0.03) were significantly associated with the risk of MV demand.DiscussionOur identification of the microbial etiology of pneumonia in MG patients may provide future perspectives on accurate antibiotic options and enable early interventions when risk factors are present.

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Section: Discussionmentioning

confidence: 99%

Pneumonia in myasthenia gravis: Microbial etiology and clinical management

Jin

Jiao

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Therefore, techniques to reliably guide the correct antimicrobial treatment in order to get the sterilization of the affected tissues and decrease the mortality are needed (8). NGS has recently emerged as a comprehensive method for exploring causative agents of infectious diseases without prior culture.However, reports about metagenomic analysis for pathogen identification in hearth valve tissues are scarce, and each of them consists of very few patients (25-34). Here, we used 16S rRNA targeted-NGS from hearth valve tissues as an approach to the diagnosis of IE in a cohort of 27 patients.…”

Section: Discussionmentioning

confidence: 99%

“…NGS has recently emerged as a comprehensive method for exploring causative agents of infectious diseases without prior culture. However, reports about metagenomic analysis for pathogen identification in hearth valve tissues are scarce, and each of them consists of very few patients (25)(26)(27)(28)(29)(30)(31)(32)(33)(34). Here, we used 16S rRNA targeted-NGS from hearth valve tissues as an approach to the diagnosis of IE in a cohort of 27 patients.…”

Section: Discussionmentioning

confidence: 99%

What does 16S rRNA gene-targeted next generation sequencing contribute to the study of infective endocarditis in valve tissue?

Santibáñez

Portillo

Santibáñez

et al. 2021

Preprint

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Infective endocarditis (IE) is a severe and life-threatening disease. Identification of infectious etiology is essential for establishing the appropriate antimicrobial treatment and decreasing mortality. The aim of this study was to explore potential utility of metagenomics for improving microbiological diagnosis of IE. In this work, next-generation sequencing (NGS) of V3-V4 region of the 16S rRNA gene was performed in 27 heart-valve tissues (18 natives, 5 intravascular devices, and 4 prosthetics) of patients diagnosed by IE. Initial microbiological diagnosis, blood culture (BC) and/or PCR, was compared with NGS-based diagnosis. Metagenomics matched with conventional techniques diagnosis in 24/27 cases (88.9%). The same bacterial family was assigned to 24 cases, the same genus to 23 cases, and the same specie for 13 cases. In 22 of them, the etiological agent was represented by percentages >99% of the reads and in two by ∼70%. Staphylococcus aureus was detected in a previously undiagnosed patient, making the microbiological diagnosis possible in one more sample than with previously used techniques. The remaining two patients showed no coincidence between traditional and NGS microbiological diagnoses. Minority records verified mixed infections in four cases and suggested confections in two cases, supported by clinical data. In conclusion: 16S rRNA gene-targeted NGS allowed to diagnose one case of IE without microbiological entity based on traditional techniques. However, the application of metagenomics to the study of IE in resected heart valves provides no benefits in comparison with BC and/or PCR. More studies are needed before implementation of NGS for the diagnosis of IE.

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