Precise Construction of Cell-Instructive 3D Microenvironments by Photopatterning a Biodegradable Hydrogel

Cai, Zhengwei; Huang, Kai-Ping; Bao, Chunyan; Wang, Xuebin; Sun, Xiang‐Chao; Xia, Hong; Lin, Qiuning; Yang, Yi; Zhu, Linyong

doi:10.1021/acs.chemmater.9b00706

Cited by 45 publications

(31 citation statements)

References 39 publications

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“…Inspired by the reaction between thiolated polymers and cysteine‐rich subdomains of the glycoprotein of intestinal mucus, [ 25 , 26 ] the thiolated‐HA polymer was prepared according to a previous report (Figure S1 , Supporting Information). [ 27 ] The successful synthesis of HA‐SH was testified by proton nuclear magnetic resonance ( 1 H NMR) and Fourier transform infrared spectroscopy (FTIR) (Figure S2 , Supporting Information; Figure 1 a ). The gas‐shearing method was applied to manufacture homogeneous microspheres on a large scale.…”

Section: Resultsmentioning

confidence: 99%

Colon‐Targeted Adhesive Hydrogel Microsphere for Regulation of Gut Immunity and Flora

et al. 2021

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Intestinal immune homeostasis and microbiome structure play a critical role in the pathogenesis and progress of inflammatory bowel disease (IBD), whereas IBD treatment remains a challenge as the first-line drugs show limited therapeutic efficiency and great side effect. In this study, a colon-targeted adhesive core-shell hydrogel microsphere is designed and fabricated by the ingenious combination of advanced gas-shearing technology and ionic diffusion method, which can congregate on colon tissue regulating the gut immune-microbiota microenvironment in IBD treatment. The degradation experiment indicates the anti-acid and colon-targeted property of the alginate hydrogel shell, and the in vivo imaging shows the mucoadhesive ability of the thiolated-hyaluronic acid hydrogel core of the microsphere, which reduces the systematic exposure and prolongs the local drug dwell time. In addition, both in vitro and in vivo study demonstrate that the microsphere significantly reduces the secretion of pro-inflammatory cytokines, induces specific type 2 macrophage differentiation, and remarkably alleviates colitis in the mice model. Moreover, 16S ribosomal RNA sequencing reveals an optimized gut flora composition, probiotics including Bifidobacterium and Lactobacillus significantly augment, while the detrimental communities are inhibited, which benefits the intestinal homeostasis. This finding provides an ideal clinical candidate for IBD treatment.

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Section: Resultsmentioning

confidence: 99%

Colon‐Targeted Adhesive Hydrogel Microsphere for Regulation of Gut Immunity and Flora

et al. 2021

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“…If a material cannot penetrate air and water, this process will be artificially blocked, resulting in the deterioration of the microenvironment. [ 36 ] Therefore, the air and water permeability of Scaffold can further improve the microenvironment of the cells after fracture and protect the respiration and physiological functions of the cells.…”

Section: Resultsmentioning

confidence: 99%

Electrospun Fibers Improving Cellular Respiration via Mitochondrial Protection

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et al. 2021

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Cellular respiration is the prerequisite for cell survival and functions, and mitochondrial function and microcirculation oxygen supply are essential for cellular respiration. However, in diabetic fracture, cellular respiration of bone marrow stem cells (BMSCs) is disrupted because of the dysfunction of mitochondria and microcirculation disorders. Here, the electrospun fibers of GelMA loaded with Hif‐1 pathway activator (DFO) are constructed to improve the cellular respiration of BMSCs via protecting mitochondrial function and reconstructing microcirculation. The sequential process of electrospinning and UV crosslinking endowed the electrospun fibers with breathability and the biomechanical properties like the periosteum. In vitro biomolecular experiments showed that by crosslinking grafted polyethylene glycol acrylate liposomes loaded with DFO, the functional electrospun fibers can release DFO locally to activate Hif‐1 in BMSCs, which can regulate the balance of Bcl‐2/Bax to protect mitochondria and upregulate the expression of VEGF to reconstruct microcirculation. Animal experiments confirmed that the functional electrospun fibers can promote the recovery of diabetic fracture in vivo. These suggested that the functional electrospun fibers can improve cellular respiration for cell survival and functions of BMSCs. This study provides a new treatment strategy for diabetic fracture and other tissue regeneration on basis of cellular respiration improvement.

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“…Furthermore, photoactivated materials can enhance cellular systems guidance into 3D microenvironments found in physiological tissues. 22,143 Photouncaging refers to the photolysis of the caged compound (Figure 9A). Specifically, photoremovable protecting groups (PPGs) have been applied to on-demand activate substrates within the hydrogel for reactions with proteins 55,144 or to liberate functional chemical groups on polymer chains, such as thiols, enabling their further click-reaction with a target (bio)molecule.…”

Section: Photodegradation and Photouncaging Reactionsmentioning

confidence: 99%

Guiding Lights: Tissue Bioprinting Using Photoactivated Materials

et al. 2020

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Photo-activated materials have found widespread use in biological and medical applications and are playing an increasingly important role in the nascent field of three-dimensional (3D) bioprinting. Light can be used as a trigger to drive the formation or the degradation of chemical bonds, leading to unprecedented spatiotemporal control over a material's chemical, physical, and biological properties. With resolution and construct size ranging from nanometres to centimeters, light-mediated biofabrication allows multicellular and multimaterial approaches. It promises to be a powerful tool to mimic the complex multiscale organization of living tissues including skin, bone, cartilage, muscle, vessels, heart, and liver, among others, with increasing organotypic functionality. With this review, we comprehensively discuss photochemical reactions, photo-activated materials, and their use in state-of-the-art deposition-based (extrusion and droplet) and vat polymerization-based (one-and two-photon) bioprinting. By offering an up-to-date view on these techniques, we identify emerging trends, focusing on both the chemistry and instrument aspects, thereby allowing the readers to select the best-suited approach. Starting with photochemical reactions and photo-activated materials, we then discuss principles, applications, and limitations of each technique. With a critical eye to the most recent achievements, the reader is guided through this exciting, emerging field with special emphasis on cell-laden hydrogel constructs.

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Precise Construction of Cell-Instructive 3D Microenvironments by Photopatterning a Biodegradable Hydrogel

Cited by 45 publications

References 39 publications

Colon‐Targeted Adhesive Hydrogel Microsphere for Regulation of Gut Immunity and Flora

Colon‐Targeted Adhesive Hydrogel Microsphere for Regulation of Gut Immunity and Flora

Electrospun Fibers Improving Cellular Respiration via Mitochondrial Protection

Guiding Lights: Tissue Bioprinting Using Photoactivated Materials

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