Precise calibration of equilibrium oxygen isotope fractionations between dissolved phosphate and water from 3 to 37 °C

Chang, Sae Jung; Blake, Ruth E.

doi:10.1016/j.gca.2014.10.030

Cited by 123 publications

(140 citation statements)

References 57 publications

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“…Recalculated data plotted in Figs. 3B and 4B, show close agreement with expected equilibrium fractionations based on dissolved inorganic PO 4 −water systems (43). We recognize the apparent temperature dependence of PO 4 −water fractionations seen in Figs.…”

Section: Recalculation Of Equilibrium Po 4 −Water Fractionations Withsupporting

confidence: 84%

“…1 and Fig. S1) (42,43). Previous experiments using the purified cell-free PPase enzyme, inorganic PO 4 , and water firmly demonstrate that the oxygen in dissolved inorganic PO 4 (DIP) is rapidly and completely (100%) exchanged with oxygen in ambient water in the medium (Fig.…”

Section: Intracellular Water Isotopic Compositionmentioning

confidence: 54%

“…1). Thus, PO 4 δ 18 O values serve as a proxy for intracellular water due to the 100% transfer of water O to PO 4 , and also due to the consistent relationship between PO 4 and water δ 18 O values that has been demonstrated by controlled laboratory experiments on PPase catalyzed PO 4 − water oxygen isotope exchange (42,43).…”

Section: Intracellular Water Isotopic Compositionmentioning

confidence: 83%

“…In contrast to previous studies reporting direct measurement of intracellular water from only one microbial strain, E. coli (27,36), we tested several different strains of bacteria to look for species-/strain-specific effects and also used the technique of multilabeled water isotope probing (MLWIP), used in previous studies of metabolic water in rats (30) and further developed in our group to probe organophosphorus and phosphoenzyme reaction mechanisms (23,(42)(43)(44). Using this technique, multiple (three or more) labeled-water probes having different oxygen isotopic composition are used to interrogate reaction mechanisms and trace oxygen transfers between compounds in reactions.…”

Section: Intracellular Water Isotopic Compositionmentioning

confidence: 99%

“…Because, according to our hypothesis, intracellular dissolved PO 4 −water fractionations are expected to reflect equilibrium PO 4 −water exchange catalyzed by intracellular PPase, δ 18 O p and δ 18 O WATER data were evaluated by comparing observed PO 4 − water oxygen isotope fractionations (1,000 ln α (p-w) ) to calculated equilibrium fractionations based on our recent calibrations based on PPase-catalyzed equilibrium exchange in the more relevant, dissolved PO 4 −water system (43). Again, assuming that intracellular water has the same isotopic composition as extracellular water, as shown in Figs.…”

Section: Intracellular Water Isotopic Compositionmentioning

confidence: 99%

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Probing the metabolic water contribution to intracellular water using oxygen isotope ratios of PO ₄

Wang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Knowledge of the relative contributions of different water sources to intracellular fluids and body water is important for many fields of study, ranging from animal physiology to paleoclimate. The intracellular fluid environment of cells is challenging to study due to the difficulties of accessing and sampling the contents of intact cells. Previous studies of multicelled organisms, mostly mammals, have estimated body water composition-including metabolic water produced as a byproduct of metabolism-based on indirect measurements of fluids averaged over the whole organism (e.g., blood) combined with modeling calculations. In microbial cells and aquatic organisms, metabolic water is not generally considered to be a significant component of intracellular water, due to the assumed unimpeded diffusion of water across cell membranes. Here we show that the 18 O/ 16 O ratio of PO 4 in intracellular biomolecules (e.g., DNA) directly reflects the O isotopic composition of intracellular water and thus may serve as a probe allowing direct sampling of the intracellular environment. We present two independent lines of evidence showing a significant contribution of metabolic water to the intracellular water of three environmentally diverse strains of bacteria. Our results indicate that ∼30-40% of O in PO 4 comprising DNA/biomass in early stationary phase cells is derived from metabolic water, which bolsters previous results and also further suggests a constant metabolic water value for cells grown under similar conditions. These results suggest that previous studies assuming identical isotopic compositions for intracellular/extracellular water may need to be reconsidered. metabolic water | isotope probing | phosphate oxygen isotopes M etabolic water, more precisely defined as an isotopically distinct flux of O (and H) produced during metabolism (1), has been studied extensively as an alternative water source contributing to body water in animals, such as desert mammals, insects, and migrating birds (2-6), but does not easily lend itself to direct measurement. In recent years, interest in metabolic water has been extended to its oxygen isotopic composition ( 18 O/ 16 O ratio or δ 18 O value) and contribution to body water because this information is crucial to the interpretation of biomineral [e.g., carbonate-CaCO 3 -and phosphate-Ca 3 (PO 4 ) 2 ] oxygen isotopic compositions used heavily in paleoclimate/paleohydrological research (7)(8)(9)(10)(11)(12)(13)(14). This includes biomineral shells of aquatic marine organisms that are preserved in the geologic record and used to infer Earth's climate history (15)(16)(17)(18)(19).A core assumption of applications of biomineral oxygen isotopic compositions to infer environmental conditions is that the 18 O: 16 O ratio is controlled by exchange of oxygen isotopes between oxyanions comprising the biomineral and ambient water in bodily fluids (i.e., body water) (9,(20)(21)(22)(23)(24). In multicellular eukaryotic organisms, body water includes all water found in various body compartments (...

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Section: Recalculation Of Equilibrium Po 4 −Water Fractionations Withsupporting

confidence: 84%

Section: Intracellular Water Isotopic Compositionmentioning

confidence: 54%

Section: Intracellular Water Isotopic Compositionmentioning

confidence: 83%

Section: Intracellular Water Isotopic Compositionmentioning

confidence: 99%

Section: Intracellular Water Isotopic Compositionmentioning

confidence: 99%

See 3 more Smart Citations

Probing the metabolic water contribution to intracellular water using oxygen isotope ratios of PO ₄

Wang

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Phosphorus availability and turnover in the Chesapeake Bay: Insights from nutrient stoichiometry and phosphate oxygen isotope ratios

Bai

Bear

et al. 2017

JGR Biogeosciences

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Understanding phosphorus (P) availability and its control on eutrophication in the Chesapeake Bay is complicated by variable sources and biogeochemical reactions transforming P forms. We investigated seasonal and spatial variability in P limitation and biological utilization in the Bay using nutrient stoichiometry (of both dissolved and particulate forms), phosphate oxygen isotope ratios, and alkaline phosphatase activity at three sites along the salinity gradient. We demonstrate that particulate nutrient ratios can be used as indicators of nutrient limitation in the Bay and suggest strong seasonal and spatial variability in P availability: the surface water is P limiting in spring, but this condition is alleviated in summer and in the deeper waters. Variability in P limitation is well reflected in the trends of phosphate oxygen isotope composition (δ18OP), with values approaching isotopic equilibrium under P limiting conditions, suggesting rapid biological P turnover. Furthermore δ18OP values suggest multiple phosphate sources including remobilization of terrestrial inorganic P phases and remineralization of organic P and P from both sources is sufficiently cycled by microorganisms, suggested by the extensive equilibrium oxygen isotope exchange. Our results further suggest high P utilization in the deeper euphotic zone where nutrients are abundant, raising caution on studying nutrient availability and limitation only in the surface water.

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Linking Sources, Transformation, and Loss of Phosphorus in the Soil–water Continuum in a Coastal Environment

Jaisi¹,

Mingus²,

Joshi³

et al. 2022

Multi‐Scale Biogeochemical Processes in Soil Ecosystems

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Precise calibration of equilibrium oxygen isotope fractionations between dissolved phosphate and water from 3 to 37 °C

Cited by 123 publications

References 57 publications

Probing the metabolic water contribution to intracellular water using oxygen isotope ratios of PO ₄

Probing the metabolic water contribution to intracellular water using oxygen isotope ratios of PO ₄

Phosphorus availability and turnover in the Chesapeake Bay: Insights from nutrient stoichiometry and phosphate oxygen isotope ratios

Linking Sources, Transformation, and Loss of Phosphorus in the Soil–water Continuum in a Coastal Environment

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Precise calibration of equilibrium oxygen isotope fractionations between dissolved phosphate and water from 3 to 37 °C

Cited by 123 publications

References 57 publications

Probing the metabolic water contribution to intracellular water using oxygen isotope ratios of PO 4

Probing the metabolic water contribution to intracellular water using oxygen isotope ratios of PO 4

Phosphorus availability and turnover in the Chesapeake Bay: Insights from nutrient stoichiometry and phosphate oxygen isotope ratios

Linking Sources, Transformation, and Loss of Phosphorus in the Soil–water Continuum in a Coastal Environment

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Probing the metabolic water contribution to intracellular water using oxygen isotope ratios of PO ₄

Probing the metabolic water contribution to intracellular water using oxygen isotope ratios of PO ₄