Precise and systematic survey of the efficacy of multicomponent drugs against functional dyspepsia

Abstract

Functional dyspepsia (FD) is one of the most prevalent functional gastrointestinal disorders, and more and more multicomponent drugs represented by traditional Chinese medicines have provided a favorable therapeutic effect in its treatment. However, their precise localization in the clinic, as well as corresponding mechanism, is ambiguous, thus hindering their widespread use. To meet this requirement, a precise and systematic approach based on a restriction of special disease-related molecules and the following network pharmacology analysis was developed and applied to a multicomponent conventional drug, XiaoErFuPi (XEFP) granules. Experimental verification of the results indicates that this approach can facilitate the prediction, and the precise and systematic efficacy of XEFP could be easily revealed, which shows that XEFP has an advantage over the positive control drug on lactate, gastrin, interleukin 4 and calcitonin gene-related peptide. Moreover, by the proteomics analysis, its superposition of multi-target effects was revealed and a new candidate target for the treatment of FD, striatin, was obtained and verified. This study provides a practicable precise approach for the investigation of the efficacy of multicomponent drugs against FD and offers a promising alternative for the systematical management of FD.

Systematic Investigation of the Efficacy of Sinitang Decoction Against Ulcerative Colitis

“…The MS analysis for QE HF was performed with one full scan (300–1,400 m/z, R=120,000 at 400 m/z) at automatic gain control target of 5×105 ions, followed by up to 30 data-dependent MS/MS scans with higher-energy collision dissociation (target 5×105 ions, max injection time 35 ms, normalized collision energy of 32%), detected in the Orbitrap. The dynamic exclusion of previously acquired precursor ions was enabled at 18 s ( Wei et al, 2019 ).…”

Role of gut microbiota via the gut-liver-brain axis in digestive diseases

“…At the same time, studies have found that Xiangsha Liujunzi Decoction alleviates the symptoms of FD by increasing the production of ghrelin, cholecystokinin and VIP and increasing the levels of these neuropeptides in the circulation[ 89 ]. Moreover, Wei et al[ 90 ] found that XiaoErFuPi granules can increase the levels of motilin, gastrin and gastric emptying rate, and thus have a good effect on patients with FD. Xue et al[ 91 ] isolated and purified magnoloside A (MA) from Magnolia officinalis, and found that MA accelerated the delayed intestinal emptying of FD rats and increased the levels of gastrin, motilin, and calcitonin gene-related proteins, reducing the levels of serotonin, nitric oxide synthase, and VIP.…”

Licorice Ameliorates Cisplatin-Induced Hepatotoxicity Through Antiapoptosis, Antioxidative Stress, Anti-Inflammation, and Acceleration of Metabolism

“…Default settings were used for variable and fixed modifications (variable modification: acetylation (protein-N terminus) and oxidation methionine (M)), fixed modification: carbamidomethylation (C). A MaxQuant label-free quantification (LFQ) algorithm was used to quantitate the MS signals, and the intensities of proteins were represented in intensity based absolute protein quantification (iBAQ) (Wei et al, 2019b). The iBAQ of each sample was transferred into a fraction of total protein iBAQ amount per experiment (FOT).…”