Precipitation of nucleic acids with cetyltrimethylammonium bromide: A method for preparing viral and cellular DNA polymerase products for cesium sulfate density gradient analysis

Reitz, Marvin S.; Abrell, John W.; Trainor, Cecelia D.; Gallo, Robert C.

doi:10.1016/0006-291x(72)90005-8

Cited by 37 publications

(17 citation statements)

References 3 publications

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“…wt. radioactivity by precipitation with cetyltrimethylammonium bromide (Reitz et al, 1972) and its size was analysed by gel electrophoresis (J. Tu, personal communication) and subsequent autoradiography (data not shown).…”

Section: Methodsmentioning

confidence: 99%

Three Strains of European Foot-and-Mouth Disease Virus are Highly Conserved in the 3'-Termini and Highly Variable in the Genes of Two Capsid Proteins

Marquardt

1982

Journal of General Virology

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SUMMARYRestriction enzyme-generated subgenomic fragments of cloned cDNA prepared from RNA of the strain O1 Kaufbeuren (OIK) of foot-and-mouth disease virus (FMDV) were compared qualitatively and quantitatively for sequence complementarity with radioactive RNA from strains C Oberbayern (Cobb) and A2 Spain (AES) in hybridization experiments on nitrocellulose membranes. Quantitative comparison of nucleic acid sequences neighbouring (Cobb/OtK) or including (A2S/OtK) the 3' end of the virus genomes demonstrated more than 80% homology. In contrast, sequences coding for the capsid proteins VP1 (10%, Cobb/OiK; 16 to 21%, A2S/O1K) and VP3 (12%, A2S/O1K ) were remarkably heterologous. Sequences downstream from the gene for VP1, i.e. those coding for non-structural proteins, showed 23 to 51% homology to both RNAs except for the area coding for protein P56a. Here, the observed homology was 82% to Cob b and 39 to 46% to AES.

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Section: Methodsmentioning

confidence: 99%

Three Strains of European Foot-and-Mouth Disease Virus are Highly Conserved in the 3'-Termini and Highly Variable in the Genes of Two Capsid Proteins

Marquardt

1982

Journal of General Virology

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“…MY divergence from baboons taken from reference 4. VOL. 21,1977 on July 6, 2020 by guest http://jvi.asm.org/ Downloaded from African primates. However, the relationship between thermal stability and percent homology reported with cDNA from BaEV (4) was not found with RNA from BaEV (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…125I-labeled RNA was purified on CF11 cellulose (20). Cetyltrimethylammonium bromide precipitation was used as a final purification step (21). The '251-labeled RNA had an approximate specific activity of 108 cpm/,ug.…”

mentioning

confidence: 99%

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Divergence of baboon endogenous type C virogenes in primates: genomic viral RNA in molecular hybridization experiments

Donehower¹,

Wong‐Staal²,

Gillespie³

1977

J Virol

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RNA purified from two related RNA tumor viruses, one isolated from a baboon, Papio anubis, and the second from cultured blood leukocytes of a patient with acute myelogenous leukemia, was labeled with 125I and hybridized to DNA from different primates. RNA from both viruses showed maximum sequence homology with genes in baboons and little homology with genes of humans. The results confirm earlier suggestions that both viruses originated by transcription of baboon virogenes, and that one was transmitted to humans in nature. Hybridization of the viral RNA to cell DNA followed complicated kinetic patterns, indicating the presence of both repeated and infrequent virogene elements. This conclusion was verified in experiments using varied DNA:RNA ratios. It is proposed that virogenes, though composed of genes repeated 10 times or more, consist of some sequences more preferentially conserved than others. The non-uniformity of virogene sequence conservation limits the use of viral probes in studies concerning certain aspects of virogene evolution. enous type C virus obtained from baboons, Papio anubis, and was similar to BaEV-related nucleotide sequences detected in the fresh tis-932 on July 6, 2020 by guest http://jvi.asm.org/ Downloaded from patients with myelogenous leukemia. Nature (London) 262:190-194.

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“…wt. radioactivity (Reitz et al, 1972) prior to use. The hybridization was performed for 70 h at 67 °C in a reaction volume of 20/A containing 20 mM-potassium phosphate pH 7.5, 5 mM-EDTA, 400 mM-NaC1 and 0.1% SDS following preincubation at 1 I0 °C for 2 min.…”

mentioning

confidence: 99%

Evidence for Non-chromosomal Hepatitis B Virus Surface (HBsAg)- and Core Antigen (HBcAg)-specific DNA Sequences in a Hepatoma Cell Line

Marquardt

Zaslavsky

Hofschneider

1982

Journal of General Virology

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SUMMARYAs demonstrated previously, a 'beta particle' fraction isolated from the cytoplasm of PLC/PRF/5 cells contains hepatitis B virus (HBV)-specific DNA. Here, further evidence is provided that the specificity of the DNA for HBV is represented at least by sequences coding for the surface and core antigen (HBsAg and HBcAg). This was shown by two different hybridization techniques. One of them, the technique of Southern, distinguished these hybrid molecules formed from those containing HBV DNA integrated into chromosomes. The HBV-specific beta particle DNA forms two distinct bands separate from the high molecular weight cellular DNA.

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Precipitation of nucleic acids with cetyltrimethylammonium bromide: A method for preparing viral and cellular DNA polymerase products for cesium sulfate density gradient analysis

Cited by 37 publications

References 3 publications

Three Strains of European Foot-and-Mouth Disease Virus are Highly Conserved in the 3'-Termini and Highly Variable in the Genes of Two Capsid Proteins

Three Strains of European Foot-and-Mouth Disease Virus are Highly Conserved in the 3'-Termini and Highly Variable in the Genes of Two Capsid Proteins

Divergence of baboon endogenous type C virogenes in primates: genomic viral RNA in molecular hybridization experiments

Evidence for Non-chromosomal Hepatitis B Virus Surface (HBsAg)- and Core Antigen (HBcAg)-specific DNA Sequences in a Hepatoma Cell Line

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