Precipitating antibodies of human antisera elicited by a purified dwarf ragweed fraction

The Adjuvant Role of Alginate on Antibody Response to Ragweed Extracts in the Rabbit

“…Pool C, prepared by DEAE-cellulose chromotography of a water soluble fraction of DMLL, possessed higher water solubility, higher allergenic activity and somewhat fewer compo nents than DMLL. Microimmunoelectrophoretic precipitin pat terns of DMLL and Pool C against a hyperimmune rabbit anti dwarf-ragweed antiserum* have been presented previously (10). DX which had been washed four times with water did not form precipitin bands against this antiserum.…”

“…To minimize variations in titer produced by different lots of erythrocytes, antisera obtained from a given bleeding and other bleedings were analyzed simultaneously. Microimmunoelectrophoretic analysis of each antiserum was performed in the manner described previously (5,10).…”

“…Alginate, per se, appears to be non-antigenic (3,4) and elicits few adverse tissue responses when used as a vehicle in hyposensitization therapy (5,6) or as an absorbable hemostatic in neuro- (2,7,8) and oral-(9) surgery. We have re ported previously the induction of precipitins and high levels of hemagglutinating antibody in the serum of two subjects that were given multiple injections of a water-soluble purified dwarf ragweed extract that was dissolved in a 4 per cent sodium alginate-0.67 per cent calcium gluconate vehicle (5,10). These explorative studies, however, were not designed to assign, unequivocally, an adjuvant role to alginate.…”

Functional Activities of γG Ragweed Antibodies Isolated by an Immunospecific Method

“…Whole antisera (in some cases decomplcmented with a heterologous immune precipitate) were selected which had relatively high haemagglutination titers of at least 2500 (tanned red cell method, ref. 4,5). Crude y-globulin fractions were obtained from whole sera by sodium sulphate precipitation and chromatographed on DEAE-cellulose by either of two methods [7,8] to obtain yG proteins.…”

“…Follow ing the demonstration of ragweed precipitating antibodies (RPAb) in whole sera of two patients who were treated with a purified small ragweed extract [4,5], we developed an immunospecific approach for the isolation of these antibodies and for similar antibodies in rabbit anti-ragweed antisera [6]. Antibodies that were obtained in this manner were qualitatively shown to possess haemagglutinating (indirect), passive cutaneous anaphylactic (in the guinea pig), and blocking (neutralizing) activities.…”