PRECIPITATING ANTIBODIES AGAINSTSTAPHYLOCOCCUS AUREUSIN SERUM FROM PATIENTS WITH STAPHYLOCOCCAL SEPTICAEMIA, INVESTIGATED BY MEANS OF QUANTITATIVE IMMUNOELECTROPHORETIC METHODS

Abstract

Espersen, F. & Hedstrom, S. A. Precipitating antibodies against Sraphylococcus aureus in serum from patients with staphylococcal septicaemia, investigated by means of quantitative immunoelectrophoretic methods. Acta path. microbiol. scand. Sect. B, 89: [261][262][263][264][265][266] 1981.Crossed immunoelectrophoresis was employed in the study of precipitating antibodies against Sraphylococcus aureus in sera from 46 patients with S. aureus septicaemia. The occurrence and titres of antibodies were compared to previously reported findings for normal persons. Ten infection-specific precipitins were detected. However, most of these precipitins were detected in low titres and could only be detected during convalescence. When antibodies against a single antigen were measured, titres above the normal level were found in 39. I % -65.2 % of the patients. When the total number of precipitins in each serum was compared to findings in serum from normal persons, 54.3% of the patients had a higher value. Most promising was the precipitin score, considering both the number of precipitins and their titres. This score showed an early rise and was increased above the normal level in 82.6% of the patients. The antibody response against individual S. nureus antigens varied from person to person both concerning titres and time course.

ANTIBODY RESPONSE TO WHOLE CELLS AND TEICHOIC ACID OF STAPHYLOCOCCUS AUREUS STRAIN E 1369 IN HUMAN SERA

“…In an attempt to identify antigens which may be useful in serology we have previously investigated the antibody response against 55 different S. aureus antigens by means of quantitative immunoelectrophoresis (3,4,6). A number of infection specific antibodies were detected but the prevalence of these and their titres were low (3,4,6). Also, the antibody response against different S.…”

“…aureus antigens showed great individual variations (6). The total antibody response against all antigens was a better parameter than the antibody response against any single antigen to differentiate between positive and negative samples (4,6). Others have used a combination of assays to increase the sensitivity and specificity (1, 10, 12).…”

Antibody Response Against Whole Staphylococcus Aureus In Patients With Staphylococcal Septicaemia and Endocarditis Investigated By ELISA

“…The development of sensitive and specific assays for detecting S. aureus antibodies in patients with S. aureus infections has been a matter of great interest for many years. Purified S. aureus cell wall antigens like peptidoglycan and teichoic acid seem to be antigens of choice for this purpose ( 1, 3, 10, 12,.14, 16, 17, 18, 19, 21), but combinations of different antigens have also been recommended (2,8,9,13). Results from different laboratories are not always in accordance.…”