Prebiotics alleviate cartilage degradation and inflammation in post-traumatic osteoarthritic mice by modulating the gut barrier and fecal metabolomics

Mi, Yanlin; YI, Nan xing; Xu, Xin; Zeng, Fang; Li, Naping; Tan, Xiaohong; Gong, Zhixian; Ke, Yan; Kuang, Gaoyan; Lü, Min

doi:10.1039/d3fo00775h

Cited by 2 publications

(3 citation statements)

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“…In vivo treatment of adrenic acid markedly prevented inflammation and alleviated the arthritis symptoms in an LTB4dependent murine arthritis model [18]. Another study demonstrated that intestinal metabolic disturbance occurred in a post-traumatic osteoarthritic (PTOA) mouse model with feces adrenic acid significantly decreased [19].…”

Section: Discussionmentioning

confidence: 97%

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A Metabolomics Study of Feces Revealed That a Disturbance of Selenium-Centered Metabolic Bioprocess Was Involved in Kashin–Beck Disease, an Osteoarthropathy Endemic to China

Wen,

Wang,

Shi

et al. 2023

Nutrients

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Background: Kashin–Beck disease (KBD) is a distinct osteoarthropathy in China with an unclear pathogenesis. This study aims to explore whether perturbations in the intestine metabolome could be linked to KBD individuals. Methods: An investigation was conducted in KBD endemic villages and fecal samples were collected. After applying inclusion and exclusion criteria, a total of 75 subjects were enrolled for this study, including 46 KBD (including 19 Grade I KBD and 27 Grade II KBD) and 29 controls. Untargeted metabolomics analysis was performed on the platform of UHPLC-MS. PLS-DA and OPLS-DA were conducted to compare the groups and identify the differential metabolites (DMs). Pathway analysis was conducted on MPaLA platform to explore the functional implication of the DMs. Results: Metabolomics analysis showed that compared with the control group, KBD individuals have a total of 584 differential metabolites with dysregulated levels such as adrenic acid (log2FC = −1.87, VIP = 4.84, p = 7.63 × 10−7), hydrogen phosphate (log2FC = −2.57, VIP = 1.27, p = 1.02 × 10−3), taurochenodeoxycholic acid (VIP = 1.16, log2FC = −3.24, p = 0.03), prostaglandin E3 (VIP = 1.17, log2FC = 2.67, p = 5.61 × 10−4), etc. Pathway analysis revealed several significantly perturbed pathways associated with KBD such as selenium micronutrient network (Q value = 3.11 × 10−3, Wikipathways), metabolism of lipids (Q value = 8.43 × 10−4, Reactome), free fatty acid receptors (Q value = 3.99 × 10−3, Reactome), and recycling of bile acids and salts (Q value = 2.98 × 10−3, Reactome). Subgroup comparisons found a total of 267 differential metabolites were shared by KBD vs. control, KBD II vs. control, and KBD I vs. control, while little difference was found between KBD II and KBD I (only one differential metabolite detected). Conclusions: KBD individuals showed distinct metabolic features characterized by perturbations in lipid metabolism and selenium-related bioprocesses. Our findings suggest that the loss of nutrients metabolism balance in intestine was involved in KBD pathogenesis. Linking the nutrients metabolism (especially selenium and lipid) to KBD cartilage damage should be a future direction of KBD study.

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Section: Discussionmentioning

confidence: 97%

“…Prebiotic treatment could exert a beneficial influence on the joint degeneration of PTOA mice, possibly through compensating for such a decrease [19]. Furthermore, adrenic acid was found to be highly correlated with femur and tibia bone mineral density (BMD) [20].…”

Section: Discussionmentioning

confidence: 99%

A Metabolomics Study of Feces Revealed That a Disturbance of Selenium-Centered Metabolic Bioprocess Was Involved in Kashin–Beck Disease, an Osteoarthropathy Endemic to China

Wen,

Wang,

Shi

et al. 2023

Nutrients

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“…In untargeted metabolomics experiments, an X500R HPLC-Q-TOF/MS (SCIEX) was used with an XSelect Premier HSS T3 column (100 mm × 2.1 mm, Waters Corporation, Milford, Massachusetts, USA) for chromatographic separation ( Jing et al, 2021 ). The mobile phase consisted of 0.1% formic acid in water (solvent A) and acetonitrile (solvent B), with a gradient duration of 18 min and a flow rate of 0.3 mL/min ( Mi et al, 2023 ). ESI was used for ionization in both positive and negative ion modes, with ion source gasses 1 and 2 set at 55 psi and curtain gas at 35 psi.…”

Section: Methodsmentioning

confidence: 99%

Puerarin alleviates inflammation and pathological damage in colitis mice by regulating metabolism and gut microbiota

Zou,

Ding,

et al. 2023

Front. Microbiol.

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Dysbiosis of gut microbiota and metabolic pathway disorders are closely related to the ulcerative colitis. Through network pharmacology, we found that puerarin is a potential ingredient that can improve the crypt deformation and inflammatory infiltration in mice, and decrease the levels of IL-1β, IL-6 and TNF-α significantly. Listeria, Alistipes and P. copri gradually became dominant bacteria in UC mice, which were positively correlated with inflammatory factors. Puerarin effectively improved dysbiosis by reducing the abundance of Alistipes, P. copri and Veillonella, and increasing the level of Desulfovibrionacea. Correlation network and metabolic function prediction analysis of the microbiota showed that they formed a tightly connected network and were widely involved in carbohydrate metabolism and amino acid metabolism. Specifically, we observed significant changes in the tryptophan metabolism pathway in DSS mice, with an increase in the abundance of Bacteroidetes and Enterobacteriaceae involved in tryptophan metabolism. However, this metabolic disorder was alleviated after puerarin treatment, including the reversal of 3-HAA levels and an increase in the abundance of Rhodobacteraceae and Halomonadaceae involved in kynurenine metabolism, as well as a significant increase in the purine metabolite guanosine. In conclusion, our study suggests that puerarin has a good therapeutic effect on UC, which is partially achieved by restoring the composition and abundance of gut microbiota and their metabolism.

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Prebiotics alleviate cartilage degradation and inflammation in post-traumatic osteoarthritic mice by modulating the gut barrier and fecal metabolomics

Abstract: Osteoarthritis (OA) is a degenerative joint disease characterized by articular cartilage degeneration, subchondral bone sclerosis, synovial hyperplasia and as the main pathological manifestations. This study aims to investigate the protective...

Cited by 2 publications

References 46 publications

A Metabolomics Study of Feces Revealed That a Disturbance of Selenium-Centered Metabolic Bioprocess Was Involved in Kashin–Beck Disease, an Osteoarthropathy Endemic to China

A Metabolomics Study of Feces Revealed That a Disturbance of Selenium-Centered Metabolic Bioprocess Was Involved in Kashin–Beck Disease, an Osteoarthropathy Endemic to China

Puerarin alleviates inflammation and pathological damage in colitis mice by regulating metabolism and gut microbiota

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