Prebiotic efficacy of coconut kernel cake’s soluble crude polysaccharides on growth rates and acidifying property of probiotic lactic acid bacteria in vitro

Abstract

This paper reports the prebiotic efficacy of soluble crude polysaccharides (SCP) from palm kernel cake on the growth rates and acidifying property of probiotic lactic acid bacteria (LAB), Lactobacillus plantarum ATCC 8014 and Lactobacillus rhamnosus ATCC 53103, to investigate into the potential SCP of an agriculture by-product as a novel prebiotic. The highest extraction yield of SCP (2.90%) was obtained using NaOH. The extracted polysaccharides were highly soluble (>97%). The highest percentage of protein (0.63 ± 0.09%) and the total carbohydrate content (55.36 ± 0.33%) were observed in NaOH-extracted SCP (SCP N) and citric acid-extracted SCP (SCP CA). Water-extracted SCP (SCP W) contained the highest percentage of glucose, whereas SCP CA demonstrated the presence of two major monosaccharides, galactose and mannose. Structural analysis of SCP showed peaks in the range of 1000-1077 cm À1 , an indication of the presence of polysaccharides and alpha (a)-or beta (b)-glycosidic linkages. The degree of hydrolysis increased with the increase of pH from 5 to 8 following 4 h incubation. SCP W and SCP CA were more resistant (95%) to a-amylase as compared to fructooligosaccharides (FOS). As in the case of FOS ($98%), SCP W ($99%) and SCP CA ($98%) were resistant to artificial human gastric juice and their degree of hydrolysis had negative correlation with the pH. The product was found to markedly stimulate the proliferation of the tested probiotics and acid production. All the above findings are supportive of the fact that polysaccharides extracted from CKC have a vast potential to be exploited as novel prebiotic.

Structural Elucidation and Antioxidant Activities of a Neutral Polysaccharide From Arecanut (Areca catechu L.)

“…PAP1a was mainly composed of mannose and galactose with a mass ratio of 3:2, and PAP1b composed of mannose, galactose, xylose, and arabinose with a molar ratio of 4.1:3.3:0.9:1.7, indicating that mannose and galactose were the main monosaccharides in neutral polysaccharides. The monosaccharide composition and proportion were similar to those of coconut soluble crude polysaccharides reported by Abbasiliasi et al (23).…”

Nutritional Profiles, Phytochemical Analysis, Antioxidant Activity and DNA Damage Protection of Makapuno Derived from Thai Aromatic Coconut

“…The dietary fiber of Makapuno meat, which was higher than that of mature coconut, was comparable to that of oat bran, chickpea, or other legumes [ 30 ]. The higher dietary fiber of Makapuno was most likely due to the accumulation of galactomannan, and thus has potential to be used as a functional food as well as in prebiotics to support gut health [ 31 , 32 , 33 , 34 ].…”

Potential of Incorporating a Functional Probiotic Encapsulant in Whipped Cream

“…The study showed that dairy dessert supplementation with whey protein concentrate improved the L. acidophilus viability during storage [33]. This could be due to the presence of polysaccharide that further produces short-chain fatty acids with probiotics' metabolic activity resulting in a decrease in pH, creating a more favorable environment for the probiotic organisms [35].…”