Prebiotic characteristics of arabinogalactans during in vitro fermentation through multi-omics analysis

Sun, Yonggan; Hu, Jielun; Zhang, Shanshan; He, Huijun; Nie, Qixing; Chen, Chunhua; Geng, Fang; Nie, Shaoping

doi:10.1016/j.fct.2021.112522

Cited by 24 publications

(13 citation statements)

References 61 publications

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“…More recently, Sun et al found a new soluble dietary fiber called Larch wood arabinogalactan (LA-AG) as a candidate prebiotic. LA-AG was capable of increasing the accumulation of organic acids by fermentation to inhibit the activity of pathogenic bacteria and improve gut health [85]. Thus, LA-AG may have the potent to the prevention of NAFLD through regulating gut-liver axis.…”

Section: Prebioticsmentioning

confidence: 99%

The Role of Gut–Liver Axis in Gut Microbiome Dysbiosis Associated NAFLD and NAFLD-HCC

Song

Zhang

2022

Biomedicines

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Nonalcoholic fatty liver disease (NAFLD) is considered as one of the most prevalent chronic liver diseases worldwide due to the rapidly rising prevalence of obesity and metabolic syndrome. As a hepatic manifestation of metabolic disease, NAFLD begins with hepatic fat accumulation and progresses to hepatic inflammation, termed as non-alcoholic steatohepatitis (NASH), hepatic fibrosis/cirrhosis, and finally leading to NAFLD-related hepatocellular carcinoma (NAFLD-HCC). Accumulating evidence showed that the gut microbiome plays a vital role in the initiation and progression of NAFLD through the gut–liver axis. The gut–liver axis is the mutual communication between gut and liver comprising the portal circulation, bile duct, and systematic circulation. The gut microbiome dysbiosis contributes to NAFLD development by dysregulating the gut–liver axis, leading to increased intestinal permeability and unrestrained transfer of microbial metabolites into the liver. In this review, we systematically summarized the up-to-date information of gut microbiome dysbiosis and metabolomic changes along the stages of steatosis, NASH, fibrosis, and NAFLD-HCC. The components and functions of the gut–liver axis and its association with NAFLD were then discussed. In addition, we highlighted current knowledge of gut microbiome-based treatment strategies targeting the gut–liver axis for preventing NAFLD and its associated HCC.

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Section: Prebioticsmentioning

confidence: 99%

The Role of Gut–Liver Axis in Gut Microbiome Dysbiosis Associated NAFLD and NAFLD-HCC

Song

Zhang

2022

Biomedicines

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“…72 Sun et al used gas chromatography (GC) to determine the organic acids in a fermentation broth in their in vitro study of the prebiotic properties of arabinogalactans, highperformance gel permeation chromatography for molecular weight analysis and gas chromatography−mass spectrometry (GC−MS) for the volatility analysis of organic metabolites, and 16S rRNA gene sequencing to analyze the intestinal microbiota cultured in vitro. 73 To study the potential prebiotic effects of fruit and vegetable byproduct (FVBP) flour in vitro, Andrade et al added FVBP flour to a medium for culturing fecal flora and extracted the DNA of the flora in the fermentation broth for real-time polymerase chain reaction (RT-qPCR), which was used to determine the composition of the microbial population, detect the production of SCFA and lactic acid in the fermentation supernatant during the culture process, and detect the effect of FVBP flour on cell viability. 74 When candidate prebiotics were selected, Yasuhiro et al used nuclear magnetic resonance (NMR)-based metabolomics methods combined with multivariate statistical analysis to study the metabolism of the intestinal microbiota and feces in vitro after incubation.…”

Section: ■ Process Of Screening Potential Prebioticsmentioning

confidence: 99%

“…When Harris et al used batch culture fermentation to determine the prebiotic activity of wheat arabinogalactan peptide, they used fluorescence in situ hybridization (FISH) to measure the type and number of bacteria in the fermentation broth and used high-performance liquid chromatography (HPLC) to measure the concentrations of SCFAs . Sun et al used gas chromatography (GC) to determine the organic acids in a fermentation broth in their in vitro study of the prebiotic properties of arabinogalactans, high-performance gel permeation chromatography for molecular weight analysis and gas chromatography–mass spectrometry (GC–MS) for the volatility analysis of organic metabolites, and 16S rRNA gene sequencing to analyze the intestinal microbiota cultured in vitro . To study the potential prebiotic effects of fruit and vegetable byproduct (FVBP) flour in vitro , Andrade et al added FVBP flour to a medium for culturing fecal flora and extracted the DNA of the flora in the fermentation broth for real-time polymerase chain reaction (RT-qPCR), which was used to determine the composition of the microbial population, detect the production of SCFA and lactic acid in the fermentation supernatant during the culture process, and detect the effect of FVBP flour on cell viability .…”

Section: Process Of Screening Potential Prebioticsmentioning

confidence: 99%

Sources, Processing-Related Transformation, and Gut Axis Regulation of Conventional and Potential Prebiotics

Cheng

Kong

Xia

et al. 2022

J. Agric. Food Chem.

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One strategy to achieve a balanced intestinal microbiota is to introduce prebiotics. Some substances present in the diet, such as soybean extracts, koji glycosylceramides, grape extracts, tea polyphenols, and seaweed extracts, can be considered as potential prebiotics, because they can selectively stimulate the proliferation of beneficial bacteria in the intestine. However, the discovery of novel prebiotics also involves advances in screening methods and the use of thermal and non-thermal processing techniques to modify and enhance the properties of beneficial organisms. The health benefits of prebiotics are also reflected by their participation in regulating the microbiota in different gut axes. In the present review, we introduced the field of prebiotics, focusing on potential prebiotic substances, the process of screening potential prebiotics, the transformation of prebiotics by food-processing technologies, and the roles of prebiotics on gut axis regulation, which, it is hoped, will promote the discovery and utilization of novel prebiotics.

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“…AG could resist the digestion in the upper digestive tract of humans and reach the colon, where it is slowly fermented by the gut microbiota (Y. Sun et al, 2021). Our previous study has shown that AG can increase body weight, reduce fasting glucose, change the relative abundance of gut microbiota, and alleviate impaired glucose tolerance in T2D rats (Nie et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Arabinogalactan, Bifidobacterium longum, and Faecalibacterium prausnitzii improve insulin resistance in high‐fat diet‐induced C57BL/6J mice

Sun

Zhang

et al. 2022

eFood

Self Cite

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The occurrence and development of insulin resistance (IR) are usually related to the change of gut microbiota. Our previous study showed that arabinogalactan (AG) could selectively increase the relative abundance of Bifidobacterium longum and Faecalibacterium prausnitzii in in vitro fermentation. However, their effect on IR remains unclear. In the current study, we investigated the effects of AG, B. longum, F. prausnitzii and their combinations on insulin-resistant mice. Intake of AG and those two strains reduced the body weight, fat accumulation, and homeostasis model assessment of IR of the mice with IR, and decreased the level of serum glucose, insulin, and lipids. Meanwhile, they could alleviate the injury of the liver. Moreover, AG, B. longum, and F. prausnitzii regulated the production of short-chain fatty acids and elevated the abundance of gut probiotics such as Lactobacillus, Lactococcus, and Bifidobacterium. However, the effect of giving only AG or a single strain was weaker than the combination of AG, B. longum, and F. prausnitzii. Our study attempted to find a new way to prevent the development of type 2 diabetes in the early stage and provided evidence for new therapy against IR with a combination of prebiotics and two bacterial strains of gut origin.

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Prebiotic characteristics of arabinogalactans during in vitro fermentation through multi-omics analysis

Cited by 24 publications

References 61 publications

The Role of Gut–Liver Axis in Gut Microbiome Dysbiosis Associated NAFLD and NAFLD-HCC

The Role of Gut–Liver Axis in Gut Microbiome Dysbiosis Associated NAFLD and NAFLD-HCC

Sources, Processing-Related Transformation, and Gut Axis Regulation of Conventional and Potential Prebiotics

Arabinogalactan, Bifidobacterium longum, and Faecalibacterium prausnitzii improve insulin resistance in high‐fat diet‐induced C57BL/6J mice

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