Preanalytical Storage Does Not Affect 99th Percentile Cardiac Troponin T Concentrations Measured with a High-Sensitivity Assay

Gillis, Judith; Dunselman, Peter H.J.M.; Jarausch, Jochen; Jong, N. de; Cobbaert, Christa

doi:10.1373/clinchem.2012.192211

Cited by 14 publications

(7 citation statements)

References 5 publications

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“…CTnT is known to be stable for 3 months at −20°C and only 9/99 samples were stored >3 months (maximum 5.5 months). Therefore, the influence of storage on our results is most likely minimal . Thirdly, it could be argued that extensive muscle damage caused troponin increase in horses with atypical myopathy.…”

Section: Discussionmentioning

confidence: 92%

Cardiac Troponin I as Compared to Troponin T for the Detection of Myocardial Damage in Horses

Vekens

Decloedt

Ven

et al. 2015

Veterinary Internal Medicne

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BackgroundDifferent cardiac troponin I (cTnI) assays give different results. Only 1 manufacturer has marketed troponin T (cTnT) assays. Therefore, cTnT often is preferred for detection of myocardial infarction in human patients. Studies of cTnT in horses are limited.ObjectivesTo compare a cTnI and a high‐sensitive cTnT assay (hs‐cTnT) in horses.AnimalsCardiac troponin I and cTnT were determined in 35 healthy horses (group 1), 23 horses suspected to have primary myocardial damage (group 2a), and 41 horses with secondary myocardial damage caused by structural heart disease (group 2b).MethodsAll cTnI samples were analyzed at laboratory A (limit of detection [LOD]: 0.03 ng/mL), whereas cTnT samples were analyzed at 2 laboratories with the same hs‐cTnT assay (laboratory B, LOD: 10.0 pg/mL; laboratory C, LOD: 4.0 pg/mL).ResultsThe median cTnI concentration in group 2a (0.90 ng/mL; range, 0.03–58.27 ng/mL) was significantly higher (P < .001) than in group 1 (0.03 ng/mL; range, 0.03–0.09 ng/mL) or group 2b (0.05 ng/mL; range, 0.03–30.92 ng/mL), and the optimal cut‐off for detection of primary myocardial damage was 0.095 ng/mL (sensitivity: 90.5%, specificity: 100%). Using an LOD of 10.0 pg/mL for all cTnT samples, a cut‐off value of 10.5 pg/mL was found, but sensitivity was low (42.9%). When only samples analyzed at laboratory C (n = 58) were included, a cut‐off of 6.6 pg/mL was found (sensitivity: 81%, specificity: 100%).Conclusions and Clinical ImportanceDespite large quantitative differences, cTnI and cTnT are both useful for detection of myocardial damage in horses.

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Section: Discussionmentioning

confidence: 92%

Cardiac Troponin I as Compared to Troponin T for the Detection of Myocardial Damage in Horses

Vekens

Decloedt

Ven

et al. 2015

Veterinary Internal Medicne

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“…Another limitation of the study is that limited data exists on the in-vitro stability of hs-cTnI and hs-cTnT. While studies have shown that hs-cTnT concentrations are stable after storage times of up to 3 months, no data exists that analyzes cTnT stability after up to 82 months [ 40 , 41 ]. Data on in vitro stability of cTnI is scarce as well, with a recent study showing a stable hs-cTnI after up to 3 months and a large study showing a stable and cTnI after 3 years [ 9 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Serum Cardiac Troponin-I is Superior to Troponin-T as a Marker for Left Ventricular Dysfunction in Clinically Stable Patients with End-Stage Renal Disease

Buiten

Bie

Rotmans³

et al. 2015

PLoS ONE

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BackgroundSerum troponin assays, widely used to detect acute cardiac ischemia, might be useful biomarkers to detect chronic cardiovascular disease (CVD). Cardiac-specific troponin-I (cTnI) and troponin-T (cTnT) generally detect myocardial necrosis equally well. In dialysis patients however, serum cTnT levels are often elevated, unlike cTnI levels. The present study aims to elucidate the associations of cTnI and cTnT with CVD in clinically stable dialysis patients.MethodsTroponin levels were measured using 5th generation hs-cTnT assays (Roche) and STAT hs-cTnI assays (Abbott) in a cohort of dialysis patients. Serum troponin levels were divided into tertiles with the lowest tertile as a reference value. Serum troponins were associated with indicators of CVD such as left ventricular mass index (LVMI), left ventricular ejection fraction (LVEF) and the presence of coronary artery disease (CAD). Associations were explored using regression analysis.ResultsWe included 154 consecutive patients, 68±7 years old, 77% male, 70% hemodialysis. Median serum cTnT was 51ng/L (exceeding the 99th percentile of the healthy population in 98%) and median serum cTnI was 13ng/L (elevated in 20%). A high cTnI (T3) was significantly associated with a higher LVMI (Beta 31.60; p=0.001) and LVEF (Beta -4.78; p=0.005) after adjusting for confounders whereas a high serum cTnT was not. CAD was significantly associated with a high cTnT (OR 4.70 p=0.02) but not with a high cTnI. Unlike cTnI, cTnT was associated with residual renal function (Beta:-0.09; p=0.006).ConclusionIn the present cohort, serum cTnI levels showed a stronger association with LVMI and LVEF than cTnT. However, cTnT was significantly associated with CAD and residual renal function, unlike cTnI. Therefore, cTnI seems to be superior to cTnT as a marker of left ventricular dysfunction in asymptomatic dialysis patients, while cTnT might be better suited to detect CAD in these patients.

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“…Plasma was stored at -80 °C until assayed. Hs-cTnT has been shown to be stable under these conditions [22].…”

Section: Specimen Collection Storage and Analysismentioning

confidence: 99%

Biological variation of high sensitivity cardiac troponin-T in stable dialysis patients: implications for clinical practice

Fahim

Hayen

Horvath

et al. 2016

Clinical Chemistry and Laboratory Medicine (CCLM)

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Preanalytical Storage Does Not Affect 99th Percentile Cardiac Troponin T Concentrations Measured with a High-Sensitivity Assay

Cited by 14 publications

References 5 publications

Cardiac Troponin I as Compared to Troponin T for the Detection of Myocardial Damage in Horses

Cardiac Troponin I as Compared to Troponin T for the Detection of Myocardial Damage in Horses

Serum Cardiac Troponin-I is Superior to Troponin-T as a Marker for Left Ventricular Dysfunction in Clinically Stable Patients with End-Stage Renal Disease

Biological variation of high sensitivity cardiac troponin-T in stable dialysis patients: implications for clinical practice

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