Preanalytical standardization of sphingosine-1-phosphate, sphinganine-1-phosphate and sphingosine analysis in human plasma by liquid chromatography–tandem mass spectrometry

Ceglarek, Uta; Dittrich, J; Helmschrodt, Christin; Wagner, Kristin; Nofer, Jerzy–Roch; Thiery, Joachim; Becker, Stefan

doi:10.1016/j.cca.2014.04.010

Cited by 15 publications

(14 citation statements)

References 22 publications

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“…Interestingly, circulating S1P concentration of the patients of this tertile were comparable with levels in a healthy population [11]. Low SA1P levels were associated with higher three-month and one-year mortality comparing survivors with non-survivors.…”

Section: Discussionmentioning

confidence: 83%

“…Although we could show that sphingolipid metabolites may serve as valid mortality predictors in this study, we think that these parameters do not have the potential to serve as supplemental biomarkers in clinical routine analysis due to their sophisticated preanalytical conditions [11]. …”

Section: Discussionmentioning

confidence: 99%

“…Sample preparation and analyte quantification were performed according to our previously published method [11]. In brief, 15 μL of citrated plasma and 85 μL of methanol including the internal standards at concentrations of 11.8 ng/mL for C17-SPH and 588 ng/mL for C17-S1P were mixed and centrifuged.…”

Section: Methodsmentioning

confidence: 99%

“…S1P, SA1P and SPH were quantified by multiple reaction monitoring experiments. Chromatographic and mass spectrometric parameters can be found elsewhere [11]. …”

Section: Methodsmentioning

confidence: 99%

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Low sphingosine-1-phosphate plasma levels are predictive for increased mortality in patients with liver cirrhosis

et al. 2017

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Background & aimThe association of circulating sphingosine-1-phosphate (S1P), a bioactive lipid involved in various cellular processes, and related metabolites such as sphinganine-1-phosphate (SA1P) and sphingosine (SPH) with mortality in patients with end-stage liver disease is investigated in the presented study. S1P as a bioactive lipid mediator, is involved in several cellular processes, however, in end-stage liver disease its role is not understood.MethodsThe study cohort consisted of 95 patients with end-stage liver disease and available information on one-year outcome. The median MELD (Model for end-stage liver disease) score was 12.41 (Range 6.43–39.63). The quantification of sphingolipids in citrated plasma specimen was performed after methanolic protein precipitation followed by hydrophilic interaction liquid chromatography and tandem mass spectrometric detection.ResultsS1P and SA1P displayed significant correlations with the MELD score. Patients with circulating S1P levels below the lowest tertile (110.68 ng/ml) showed the poorest one-year survival rate of only 57.1%, whereas one-year survival rate in patients with S1P plasma levels above 165.67 ng/ml was 93.8%. In a multivariate cox regression analysis including platelet counts, concentrations of hemoglobin and MELD score, S1P remained a significant predictor for three-month and one-year mortality.ConclusionsLow plasma S1P concentrations are highly significantly associated with prognosis in end-stage liver disease. This association is independent of the stage of liver disease. Further studies should be performed to investigate S1P, its role in the pathophysiology of liver diseases and its potential for therapeutic interventions.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…S1P, SA1P and SPH were quantified by multiple reaction monitoring experiments. Chromatographic and mass spectrometric parameters can be found elsewhere [11]. …”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Low sphingosine-1-phosphate plasma levels are predictive for increased mortality in patients with liver cirrhosis

et al. 2017

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“…Complete blood count was obtained using automated analyzer (Sysmex XE-2100, Dasit, Milano, Italy) at the routine hospital laboratory using a protocol calibrated for mouse blood. S1P concentration in plasma was determined using hydrophilic interaction liquid chromatography (HILIC, SeQuant™ ZIC ® -HILIC column) followed by tandem mass spectrometry as described previously [17]. Total cholesterol, HDL cholesterol and triglycerides were determined in plasma using commercially available kits (Instrumentation Laboratory Spa, Milano, Italy).…”

Section: Analytical Proceduresmentioning

confidence: 99%