Preanalytical Influences on DPC IMMULITE 2000 Intact PTH Assays of Plasma and Serum from Dialysis Patients

Holmes, Daniel T.; Levin, Adeera; Forer, Barry; Rosenberg, Frances

doi:10.1373/clinchem.2004.046821

Cited by 38 publications

(32 citation statements)

References 11 publications

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“…Unauthenticated Download Date | 5/11/18 5:34 AM EDTA plasma after 24 and 48 h were observed, although it was concluded overall that PTH was stable for 72 h in EDTA plasma but for only 24 h in serum [34]. Overall, these results suggested that PTH was stable in separated EDTA plasma for at least 48 h at room temperature but that in serum significant immunoreactivity may be lost after as little as 2 h.…”

Section: Stability In Separated Serum and Plasma At Room Temperaturementioning

confidence: 92%

“…PTH stability in EDTA plasma and serum was directly compared in seven studies using second generation assays [25,29,[31][32][33][34][35]. In these, PTH was more stable in EDTA plasma than in serum (Table 3).…”

Section: Stability In Separated Serum and Plasma At Room Temperaturementioning

confidence: 99%

“…Nine studies addressed stability at 4°C in serum or plasma (Supplemental Data Table 4) [19,22,24,25,29,33,34,38,39]. Five of these directly compared the stability of PTH in serum and plasma at 4°C [25,29,33,34,38] using second generation assays generally confirming superior stability in EDTA (Table 3). In both sample types, PTH was more stable at 4°C than at room temperature.…”

Section: Stability At Other Temperaturesmentioning

confidence: 99%

“…With respect to analyte stability ex vivo, most studies, with both second and third generation assays, indicate PTH to be more stable in EDTA whole blood than clotted whole blood [15,16,18,25,26,29,30], and in EDTA and lithium heparin plasma than in serum at room temperature [25,[31][32][33][34][35]. Whilst direct comparisons of lithium heparin whole blood with EDTA whole blood [25], and lithium heparin plasma with EDTA plasma [25,32], suggested similar stability could be achieved in lithium heparin preserved tubes, there was limited evidence upon which to base a recommendation.…”

Section: Recommendations For Sample Handlingmentioning

confidence: 99%

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Sampling and storage conditions influencing the measurement of parathyroid hormone in blood samples: a systematic review

Hanon

Sturgeon

Lamb

2013

Clinical Chemistry and Laboratory Medicine (CCLM)

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Parathyroid hormone (PTH) is relatively unstable: optimisation of pre-analytical conditions, including specimen type, sampling time and storage conditions, is essential. We have undertaken a systematic review of these pre-analytical conditions. An electronic search of the PubMed, Embase, Cochrane, Centre for Research and Dissemination and Bandolier databases was undertaken. Of 5511 papers identified, 96 underwent full text review, of which 83 were finally included. At room temperature PTH was stable in ethylenediaminetetraacetic acid (EDTA) preserved whole blood for at least 24 h and in EDTA plasma for at least 48 h after venepuncture. Losses were observed in clotted blood samples after 3 h and in serum after 2 h. At 4°C PTH was more stable in EDTA plasma (at least 72 h) than serum (at least 24 h). Central venous PTH concentrations were higher than peripheral venous concentrations. In the northern hemisphere, PTH concentrations were higher in winter than summer. PTH has a circadian rhythm characterised by a nocturnal acrophase and mid-morning nadir. Data related to frozen storage of PTH (−20°C and −80°C) were limited and contradictory. We recommend that blood samples for PTH measurement should be taken into tubes containing EDTA, ideally between 10:00 and 16:00, and plasma separated within 24 h of venepuncture. Plasma samples should be stored at 4°C and analysed within 72 h of venepuncture. Particular regard must be paid to the venepuncture site when interpreting PTH concentration. Further research is required to clarify the suitability of freezing samples prior to PTH measurement.

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Section: Stability In Separated Serum and Plasma At Room Temperaturementioning

confidence: 92%

Section: Stability In Separated Serum and Plasma At Room Temperaturementioning

confidence: 99%

Section: Stability At Other Temperaturesmentioning

confidence: 99%

Section: Recommendations For Sample Handlingmentioning

confidence: 99%

See 2 more Smart Citations

Sampling and storage conditions influencing the measurement of parathyroid hormone in blood samples: a systematic review

Hanon

Sturgeon

Lamb

2013

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…Worldwide, nephrologists rely on the Kidney Disease Outcomes Quality Initiative (KDOQI) guidelines (1) to initiate or maintain appropriate therapeutic treatments. Recently, some papers have illustrated the need for a standardization of the pre-analytical (2,3) and analytical (4,5) phases. However, to our knowledge, the stability of PTH at Ϫ80°C for a long storage period (1 yr or more) has never been studied.…”

Section: P Arathyroid Hormone (Parathormone [Pth]) Determina-mentioning

confidence: 99%

Estimation of the Stability of Parathyroid Hormone when Stored at −80°C for a Long Period

Cavalier

Hubert

Krzesinski

et al. 2009

Clinical Journal of the American Society of Nephrology

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Background and objectives: Stability of parathyroid hormone (PTH) at ؊80°C for long storage periods has never been studied. This can be of importance for the conclusions of studies where blood banks have been constituted. The study's aim was to evaluate stability of PTH when stored as serum or plasma EDTA samples at ؊80°C.Design, setting, participants & measurements: Samples were collected from 16 chronic hemodialysis patients using EDTA and gel-separator tubes. Plasma and serum were aliquoted; one aliquot was assayed with Elecsys and Liaison methods to determine the "baseline" values and another aliquot after 1, 3, 6, and 12 mo. The factors "method," "tubes," "subjects," and "time" were included in a mixed linear model to evaluate their effects on measured PTH values. The prediction interval methodology was used to assess where a future result could be obtained with a defined probability.Results: With the Liaison method, the maximum storage times with either dry or EDTA tubes were estimated to be 9 and 2 mo, respectively. With the Elecsys method, samples could be stored at least 2 yr with acceptable level of degradation.Conclusion: PTH stability at ؊80°C is not infinite. Maximum storage time and acceptance limits (30%) were defined, showing that with one method, samples should be stored for not more than 2 mo, whereas the other could be stored for up to 2 yr. With any PTH assay, the maximum storage time should be evaluated to ascertain that samples will keep their initial reactive profile after prolonged storage periods.

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Nebenschilddrüse

Schäffler¹,

Bollheimer²,

Büttner³

et al. 2012

Funktionsdiagnostik in Endokrinologie, Diabetologie Und Stoffwechsel

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Preanalytical Influences on DPC IMMULITE 2000 Intact PTH Assays of Plasma and Serum from Dialysis Patients

Cited by 38 publications

References 11 publications

Sampling and storage conditions influencing the measurement of parathyroid hormone in blood samples: a systematic review

Sampling and storage conditions influencing the measurement of parathyroid hormone in blood samples: a systematic review

Estimation of the Stability of Parathyroid Hormone when Stored at −80°C for a Long Period

Nebenschilddrüse

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