Preanalytical Factors in the Measurement of Intact Parathyroid Hormone with the DPC IMMULITE Assay

Glendenning, Paul; Musk, Alexander A.; Taranto, Mario; Vasikaran, Samuel

doi:10.1093/clinchem/48.3.566

Cited by 24 publications

(12 citation statements)

References 4 publications

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“…Intact parathyroid hormone (PTH) was measured in EDTA plasma by immunochemiluminescence on the Immulite 2000 platform, ensuring complete sample filling of the collection vessel. 27,28 Interassay CV for intact PTH was 9% at 3AE2 pmol/l, 5AE6% at 30AE3 pmol/l and 5% at 104 pmol/l. 25OHD was measured by radioimmunoassay (RIA) using the DiaSorin RIA method with interassay CV of <10% at 14 and 33 nmol/l.…”

Section: Methodsmentioning

confidence: 93%

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ORIGINAL ARTICLE: Association between 25‐hydroxyvitamin D, somatic muscle weakness and falls risk in end‐stage renal failure

Boudville

Inderjeeth

Elder

et al. 2010

Clinical Endocrinology

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Suboptimal levels of 25OHD in CKD-5D are associated with reduced quadriceps muscle strength and increased falls risk. 25OHD may be more important than the active renal metabolite 1,25(OH)(2)D for muscle strength with implications for vitamin D choice and goals of supplementation. Further investigation is required to examine effectiveness of calciferol supplementation on the incidence of falls in CKD-5D.

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Section: Methodsmentioning

confidence: 93%

“…Intact parathyroid hormone (PTH) was measured in EDTA plasma by immunochemiluminescence on the Immulite 2000 platform, ensuring complete sample filling of the collection vessel 27,28 . Interassay CV for intact PTH was 9% at 3·2 pmol/l, 5·6% at 30·3 pmol/l and 5% at 104 pmol/l.…”

Section: Methodsmentioning

confidence: 99%

ORIGINAL ARTICLE: Association between 25‐hydroxyvitamin D, somatic muscle weakness and falls risk in end‐stage renal failure

Boudville

Inderjeeth

Elder

et al. 2010

Clinical Endocrinology

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“…This effect is due to the relatively high EDTA concentration within the sample mix, which chelates ions that are critical for the alkaline phosphatase indicator enzyme used in this assay. 19 These issues need to be appreciated by clinician users of this intact PTH assay, as well as by laboratories that offer this diagnostic test. Autoantibody and heterophile antibody interference is a recognized cause of diagnostic misclassification for all immunoassays, however this is thankfully rarely seen with intact PTH assays 20 (Table 3).…”

Section: Recommendations For Australian Laboratoriesmentioning

confidence: 99%

“…Insufficient sample volume (underfilling the vessel by ≥50%) can result in falsely low intact PTH values using the DPC Immulite assay. This effect is due to the relatively high EDTA concentration within the sample mix, which chelates ions that are critical for the alkaline phosphatase indicator enzyme used in this assay 19 . These issues need to be appreciated by clinician users of this intact PTH assay, as well as by laboratories that offer this diagnostic test.…”

Section: Diagnostic Criteria Of Phptmentioning

confidence: 99%

Diagnosis of primary hyperparathyroidism: controversies, practical issues and the need for Australian guidelines

Glendenning¹

2003

Internal Medicine Journal

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Primary hyperparathyroidism (PHPT) is one of the most common endocrine disease processes, however the clinical presentation in 2003 is typically characterized by minimal signs or symptoms of hypercalcaemia or para-thyroid hormone (PTH) excess. Recent developments in imaging and management of PHPT have been published, however the area of biochemical investigation has been relatively neglected. A group of experts convened in April 2002 to consider whether changes were needed to the 1990 consensus guidelines which defined criteria for the diagnosis and management of asymptomatic PHPT. It is appropriate to review the revised recommendations, which have been disseminated by the panel and were recently published. Each of the laboratory -analytes used to establish the diagnosis of PHPT and exclude alternative diagnoses or complications will be considered in succession in this review: (i) calcium, (ii) intact PTH, (iii) urinary calcium and (iv) 25 hydroxy-vitamin D. Furthermore, critical appraisal of the new diagnostic criteria and their applicability to Australian laboratories will be addressed. Finally, limitations and problems associated with the measurement of each analyte will be reviewed.

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“…Each one used its routine laboratory kit that is used to measure intact PTH in dogs and cats (Laboratorio Echevarne, Calle Provenza, 312 Bajos, 08037 Barcelona, Spain) (Laboklin GmbH & Co. KG, Steubenstraße 4, 97688 Bad Kissingen, Germany). Blood on EDTA of two of the 8 guinea pigs randomly selected was sent at room temperature and analyzed in less than 24 h by one laboratory (Laboratorio Echevarne, Calle Provenza, 312 Bajos, 08037 Barcelona, Spain), because the PTH levels in these samples are proven to be higher than in frozen serum [ 7 , 15 , 16 ]. This analysis was performed to partially discard the influence of temperature and EDTA on the results obtained in frozen plasma.…”

Section: Methodsmentioning

confidence: 99%

Case Report of a Satin Guinea Pig with Fibrous Osteodystrophy That Resembles Human Pseudohypoparathyroidism

Gallego

2017

Case Reports in Veterinary Medicine

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A case report of a 2-year-old female satin guinea pig with a history of dental overgrowth and lameness and radiological lesions of fibrous osteodystrophy is presented. The most relevant clinical findings were bone demineralization, high level of parathyroid hormone (PTH), normophosphatemia, normal ionized calcium, and low total thyroxine (tT4) with a normal renal function. Long-term treatment was based on teeth coronal reduction and maintaining a balanced diet. PTH measurement was performed with a kit suitable for rats to test 4 different paired samples of guinea pigs and resulted in similar results for each pair of measurements. Two kits routinely employed in dogs and cats failed in measuring PTH in guinea pig serum samples. The ionized calcium, PTH, and tT4 values, not previously reported in similar cases, were obtained. The determination of tT4 could be useful in the diagnosis of fibrous osteodystrophy in guinea pigs. The observed findings show similarity with human pseudohypoparathyroidism type Ia, a disease caused by an inactivating heterozygous mutation of the stimulatory G protein α subunit from the maternal genome that induces multiple hormone resistance and that courses with a syndrome called Albright hereditary osteodystrophy. Naturally occurring pseudohypoparathyroidism in animals has been reported previously only in a ferret.

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Preanalytical Factors in the Measurement of Intact Parathyroid Hormone with the DPC IMMULITE Assay

Cited by 24 publications

References 4 publications

ORIGINAL ARTICLE: Association between 25‐hydroxyvitamin D, somatic muscle weakness and falls risk in end‐stage renal failure

ORIGINAL ARTICLE: Association between 25‐hydroxyvitamin D, somatic muscle weakness and falls risk in end‐stage renal failure

Diagnosis of primary hyperparathyroidism: controversies, practical issues and the need for Australian guidelines

Case Report of a Satin Guinea Pig with Fibrous Osteodystrophy That Resembles Human Pseudohypoparathyroidism

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