Pre-Treatment of Swine Oviductal Epithelial Cells with Progesterone Increases the Sperm Fertilizing Ability in an IVF Model

Cimini, Costanza; Moussa, Fadl; Taraschi, Angela; Ramal-Sanchez, Marina; Colosimo, Alessia; Capacchietti, Giulia; Mokh, Samia; Valbonetti, Luca; Tagaram, Israiel; Bernabò, Nicola; Barboni, Barbara

doi:10.3390/ani12091191

Cited by 4 publications

(4 citation statements)

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“…All these mechanisms help the oocytes resist the HS. Finally, deciphering the molecular biomarkers, which are correlated with GRe‐induced resistance of porcine IVM‐derived oocytes to the onset and/or progression of molecular pathways related not only to the processes of heat and oxidative stress, but also to apoptotic cell death, might contribute to the enhancements in the developmental competences and quality of porcine and other mammalian in vitro‐produced embryos propagated by such powerful tools as the ex vivo fertilization by gamete co‐incubation or intracytoplasmic sperm injection (ICSI) ((Cimini et al, 2022; Devito et al, 2010; Okada et al, 2022; Sessions‐Bresnahan et al, 2014; Viet Linh et al, 2009) and cloning by somatic cell nuclear transfer (SCNT) (Gambini et al, 2020; Opiela et al, 2017; Samiec & Skrzyszowska, 2005, 2021; Skrzyszowska et al, 2008; Vichera et al, 2010)).…”

Section: Discussionmentioning

confidence: 99%

Presence of ginsenoside re during in vitro maturation protects porcine oocytes from heat stress

Liu

Duan

et al. 2022

Reprod Domestic Animals

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Heat stress (HS) affects the development of porcine gametes and embryos negatively, induces the decrease of reproductive ability significantly, threatens global pig production. Ginsenoside Re (GRe), is a main bioactive component of ginseng, shows very specific anti‐apoptotic, antioxidant and anti‐inflammatory activities. To investigate the alleviating effect of GRe on the in vitro maturation of porcine oocyte under the HS, the polar body extrusion rate, intracellular levels of reactive oxygen species (ROS) and glutathione (GSH), ATP content, mitochondrial membrane potential (MMP) were assessed. For the current study, porcine cumulus‐oocyte complexes (COCs) randomly divided into four groups: the control, GRe, HS and HS + GRe group. The results showed that HS inhibited the cumulus cell expansion and polar body extrusion rate, the levels of GSH and MMP, the ATP content, the gene expression of Nrf2 of porcine oocytes and the parthenogenetic activation (PA) embryo development competence, but GRe treatment could partly neutralize these adverse effects. Furthermore, HS increased the ROS formation and percentage of apoptosis, the gene expression of HSP90, CASP3 and CytoC of porcine oocytes, but GRe could weaken the effect on Cyto C and BAX expression induced by HS. Taken together, these results showed that the presence of GRe during in vitro maturation protects porcine oocytes from HS. These findings lay a foundation for GRe may be used as a potential protective drug to protect porcine oocytes against HS damage.

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Section: Discussionmentioning

confidence: 99%

Presence of ginsenoside re during in vitro maturation protects porcine oocytes from heat stress

Liu

Duan

et al. 2022

Reprod Domestic Animals

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“…Aspirate the supernatant carefully and discard it. Droplets of testicular cell suspension, ten microliters of HEPES-buffered growth media, and one droplet of 10% polyvinylpyrrolidone (PVP; Sigma) were added to the injection plate.Covering the plate was filtered mineral oil (Sigma) that had undergone embryo testing [16].…”

Section: Traditional Methods For Sperm Preparationmentioning

confidence: 99%

Novel Method to Improve IVF and ICSI Outcomes

Abou El-Ela,

kandeel,

Attia

et al. 2023

Labyrinth: Fayoum Journal of Science and Interdisciplinary Stud

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Hyaluronan is a constituent of the cumulus oophorous complex, which envelops the oocyte. It is widely acknowledged as a pivotal marker for the quality and maturity of sperm. For male factors that have a high DNA Fragmentation Index (DFI), those having in vitro fertilization (IVF) or Intracytoplasmic sperm injection ICSI, those who have repeated miscarriages because of male factors, and those who lose their pregnancies early because of male factors. The current study's robust result demonstrates the effectiveness of the HA (Physiological intracytoplasmic sperm injection PICSI)) selection system. Based on available and historical data, we can surmise that sperm chosen using the HA technique prior to ICSI offer a higher predictive value for developing viable embryos, thereby aiding in the optimization of treatment outcomes. The HA binding method for sperm selection can be used in routine laboratory settings to boost conception rates and lower the risk of genetic issues by inexpensively replicating a physiologic choice of the male gamete. Clinic abortion rates have been successfully lowered by 50% with the use of this procedure for patients with oligoasthenoteratozoospermia and for couples who experienced consecutive implantation failures. The primary outcomes of this research are an increase in the quantity of blastocysts extracted each cycle, the quantity of Grade A embryos, the rate of cleavage, the quality of the embryos, the rate of implantation, and ideally the rate of pregnancy. It can be inferred that sperm selected using the HA approach before ICSI had a better chance of becoming viable embryos. The employment of HA (PICSI) media techniques in ICSI led to higher rates of fertilization and pregnancy because they help to minimize sperm with aberrant morphological flaws and promote motility, notably progressive motility. This study's recommendation is to use hyaluronan when selecting spermatozoa to produce high-quality embryos with high pregnancy rates.

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“…Total RNA was extracted using Total RNA Purification Kit (NORGEN Biotek) as previously described [ 47 , 48 ] and reported in Supplementary Materials . Quantitative real-time PCR (qPCR) was carried out using primer sequences for IRF1 , NFKb , TGRF2 , CAAT , DOK2 , RAS-GAP , and ACTB genes.…”

Section: Methodsmentioning

confidence: 99%

CD200 as a Potential New Player in Inflammation during Rotator Cuff Tendon Injury/Repair: An In Vitro Model

Giancola

Oliva

Gallorini

et al. 2022

IJMS

Self Cite

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Rotator cuff tendon (RCT) disease results from multifactorial mechanisms, in which inflammation plays a key role. Pro-inflammatory cytokines and tendon stem cell/progenitor cells (TSPCs) have been shown to participate in the inflammatory response. However, the underlying molecular mechanism is still not clear. In this study, flow cytometry analyses of different subpopulations of RCT-derived TSPCs demonstrate that after three days of administration, TNFα alone or in combination with IFNγ significantly decreases the percentage of CD146+CD49d+ and CD146+CD49f+ but not CD146+CD109+ TSPCs populations. In parallel, the same pro-inflammatory cytokines upregulate the expression of CD200 in the CD146+ TSPCs population. Additionally, the TNFα/IFNγ combination modulates the protein expression of STAT1, STAT3, and MMP9, but not fibromodulin. At the gene level, IRF1, CAAT (CAAT/EBPbeta), and DOK2 but not NF-κb, TGRF2 (TGFBR2), and RAS-GAP are modulated. In conclusion, although our study has several important limitations, the results highlight a new potential role of CD200 in regulating inflammation during tendon injuries. In addition, the genes analyzed here might be new potential players in the inflammatory response of TSPCs.

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Pre-Treatment of Swine Oviductal Epithelial Cells with Progesterone Increases the Sperm Fertilizing Ability in an IVF Model

Cited by 4 publications

References 86 publications

Presence of ginsenoside re during in vitro maturation protects porcine oocytes from heat stress

Presence of ginsenoside re during in vitro maturation protects porcine oocytes from heat stress

Novel Method to Improve IVF and ICSI Outcomes

CD200 as a Potential New Player in Inflammation during Rotator Cuff Tendon Injury/Repair: An In Vitro Model

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