Pre-treatment of Nile tilapia (Oreochromis niloticus) with ozone nanobubbles improve efficacy of heat-killed Streptococcus agalactiae immersion vaccine

Linh, Nguyen Vu; Dien, Le Thanh; Sangpo, Pattiya; Senapin, Saengchan; Thapinta, Darawan; Panphut, Wattana; St‐Hilaire, Sophie; Rodkhum, Channarong; Dong, Ha Thanh

doi:10.1016/j.fsi.2022.03.007

Cited by 15 publications

(19 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hyperosmotic method is to soak the fish in 3% ~ 5% salt solution for 5 minutes, then move the fish herd to the vaccine solution for immersing, to force the antigen to infiltrate into the fish by the change of osmotic pressure ( 248 , 266 ). Ultrasonic treatment can increase the permeability of biological tissues such as skin and muscles, and the number of antigens entering a fish’s body can be significantly increased by ultrasound in spray ( 267 , 268 ). Although hypertonic and spray methods can improve the efficacy of immersion vaccines, their application is limited by stress on fish bodies.…”

Section: Aquatic Vaccination Methodsmentioning

confidence: 99%

Current status and development prospects of aquatic vaccines

Liu

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

Diseases are a significant impediment to aquaculture’s sustainable and healthy growth. The aquaculture industry is suffering significant financial losses as a result of the worsening water quality and increasing frequency of aquatic disease outbreaks caused by the expansion of aquaculture. Drug control, immunoprophylaxis, ecologically integrated control, etc. are the principal control strategies for fish infections. For a long time, the prevention and control of aquatic diseases have mainly relied on the use of various antibiotics and chemical drugs. However, long-term use of chemical inputs not only increases pathogenic bacteria resistance but also damages the fish and aquaculture environments, resulting in drug residues in aquatic products, severely impeding the development of the aquaculture industry. The development and use of aquatic vaccines are the safest and most effective ways to prevent aquatic animal diseases and preserve the health and sustainability of aquaculture. To give references for the development and implementation of aquatic vaccines, this study reviews the development history, types, inoculation techniques, mechanisms of action, development prospects, and challenges encountered with aquatic vaccines.

show abstract

Section: Aquatic Vaccination Methodsmentioning

confidence: 99%

Current status and development prospects of aquatic vaccines

Liu

et al. 2022

Front. Immunol.

View full text Add to dashboard Cite

show abstract

“…Total RNA extracted from the gills and head kidneys were used for the specific-immune gene expression analysis. Immunoglobulin transcripts encoding IgM and IgT were selected as target genes, by using the same primer pairs and quantitative PCR (qPCR) conditions as described previously in Linh et al (2021Linh et al ( , 2022. Relative gene expression was performed by the 2 -ΔΔCt method (Livak & Schmittgen, 2001), using the EF1α expression of the AC group at the same sampling point for normalization.…”

Section: Gene Expression Analysismentioning

confidence: 99%

“…Ensuring the efficacy of a mucosal vaccine, therefore, may require their application with adjuvants or with treatments to obtain an appropriate immune response from the fish (Adams, 2019;Bøgwald & Dalmo, 2019). Recent research has found that the use of an ozone nanobubble (ONb; typically less than 130 nm) treatment can modulate the innate immune response of fish (Linh et al, 2021) and enhance the efficacy of an immersible S. agalactiae vaccine, making it a promising application for use in aquaculture (Linh et al, 2022). To prolong the vaccine efficacy, a booster dose is recommended during the grow-out culture period, and oral vaccines are considered more appealing over other delivery methods (Plumb & Vinitnantharat, 1993;Chettri et al, 2015;Schmidt et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Innovative approach for vaccinating Nile tilapia,Oreochromis niloticusagainstStreptococcus agalactiaeusing an ozone nanobubble pre-treatment, VAC in BAG and VAC in FEED

Vinh,

Dong,

Senapin

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

The treatment of Nile tilapia with ozone nanobubbles (ONb) prior to vaccination with an immersible heat-killed Streptococcus agalactiae (Sa) vaccine has been reported to modulate and enhance both innate and specific immunity. The efficacy of this novel vaccination strategy is explored further in field trials. This strategy involved a short-term treatment of ONb to activate the fish's immunity prior to immersion vaccination during their transportation in oxygenated plastic bags (VAC in BAG), followed by two oral boosters during the grow-out stage mixing vaccine in feed (VAC in FEED). The field trial was conducted over 112 days in open cages, comprising four groups: normal aeration control (AC), normal aeration + vaccine (AV), ONb control (NC), and ONb + vaccine (NV). The efficacy of the vaccine was evaluated by measuring specific antibodies for S. agalactiae, monitoring expressions of IgM and IgT transcripts in the gills and head kidney every two weeks, and a laboratory pathogen challenge. Results found that fish in the NV group had significant increases in anti-S. agalactiae antibodies after the primary dose, whereas fish in the AV group required an oral booster dose to produce significant anti-S. agalactiae antibodies. In the vaccinated groups (AV and NV), only IgM was observed to be upregulated at 14 days post-immersion (dpi), while this gene was upregulated in both gills and head kidney in the NC group. No statistically significant upregulation of IgT was recorded in any group at any time point. Despite a decline in the levels of specific antibodies among the vaccinated groups at the time of challenge (88 dpi), the NV and AV groups demonstrated a relative percent survival (RPS) of 50% and 46.7%, respectively, following a relatively high injection dose of S. agalactiae injection (0.1 mL of 108 CFU/mL). In summary, this ONb, VAC in BAG and VAC in FEED vaccination strategy represents a promising alternative to the undesirable handling and costly injection approach used within the Nile tilapia industry.

show abstract

“…While oxygen, nitrogen, and air are the most common types of gases studied as nanobubbles (NBs), research with ozone NBs is still emerging: (i) remediation of sludge, sediments, and groundwater, − (ii) removal of micropollutants, − (iii) reduction of bacterial pathogens, − and (iv) antimicrobial effectiveness for fresh vegetables . Ozone readily reacts with organic and inorganic compounds in water, and it has been widely used as an oxidant and/or disinfectant in water and wastewater treatment.…”

Section: Introductionmentioning

confidence: 99%

Characteristics and Stability of Ozone Nanobubbles in Freshwater Conditions

Soyluoglu,

Kim,

Karanfil

2023

Environ. Sci. Technol.

View full text Add to dashboard Cite

Pre-treatment of Nile tilapia (Oreochromis niloticus) with ozone nanobubbles improve efficacy of heat-killed Streptococcus agalactiae immersion vaccine

Cited by 15 publications

References 50 publications

Current status and development prospects of aquatic vaccines

Current status and development prospects of aquatic vaccines

Innovative approach for vaccinating Nile tilapia,Oreochromis niloticusagainstStreptococcus agalactiaeusing an ozone nanobubble pre-treatment, VAC in BAG and VAC in FEED

Characteristics and Stability of Ozone Nanobubbles in Freshwater Conditions

Contact Info

Product

Resources

About