Pre-treatment and inoculum affect the microbial community structure and enhance the biogas reactor performance in a pilot-scale biodigestion of municipal solid waste

Ventorino, Valeria; Romano, Ida; Pagliano, Giorgia; Robertiello, Alessandro; Pepe, Olimpia

doi:10.1016/j.wasman.2017.12.005

Cited by 46 publications

(28 citation statements)

References 70 publications

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“…Various biological or biochemical reasons have been provided by other researchers for increase in biogas yield due to inoculum. For instance, inoculum affects the anaerobic process by providing initial microbial population which plays a key role in enhancing biogas production [31]. Another reason in enhancing biogas production might be the micro and macro nutrients content of inoculum which is required by anaerobic process [32] Enzymes activity of inoculum in degradation of cellulose and hemicellulose might be another reason for enhancing biogas production [30].…”

Section: Effect Of Inoculum Type On Biogas Production and Solids Removalmentioning

confidence: 99%

Effect of inoculum type and organic loading on biogas production of sunflower meal and wheat straw

Rajput¹,

Sheikh²

2019

Sustain Environ Res

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Inoculum plays a vital role in providing initial microbial population in anaerobic process. There is unavailability of standard inoculum in the Pakistan and the available inoculum requires pretreatment and preparation before use. Thus, in this study the effect of inoculum type and organic loading (OL) on the biogas yield of sunflower meal and wheat straw was evaluated. For this purpose, lab scale batch experiments were conducted at OL of 2 g VS L − 1 in 225 mL glass bottle using digested manure, acclimatized sludge and septic tank sludge as inoculum. The highest biogas yield and volatile solids reduction of 768 NmL g − 1 VS and 78%, respectively, were observed from sunflower meal with digested manure. Reactor inoculated with digested manure also showed better buffering capacity in terms of pH. The inoculum selected from first experiment was used to study the effect of OL (2, 6, 10 and 14 g VS L − 1) on biogas yield. Results showed that biogas yield decreased with the increased in OL. Stability parameters revealed that reactors can be operated safely up to OL of 10 g VS L − 1. Biogas production data were modelled by modified Gompertz function.

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Section: Effect Of Inoculum Type On Biogas Production and Solids Removalmentioning

confidence: 99%

Effect of inoculum type and organic loading on biogas production of sunflower meal and wheat straw

Rajput¹,

Sheikh²

2019

Sustain Environ Res

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“…For inoculum, we used 30 liters of domestic wastewater from a septic tank. The batch experiment was conducted for an HRT of 30 days at mesophilic temperature with continuous parameter monitoring to observe the influence of the temperature and pH on biogas production [15,17,20,21,40,45,46,[50][51][52][53]. The reactor operated under mesophilic conditions with leachate recirculation.…”

Section: Experiments Nomentioning

confidence: 99%

Pilot-Scale Anaerobic Co-Digestion of the OFMSW: Improving Biogas Production and Startup

et al. 2018

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This paper presents experimental results regarding anaerobic co-digestion of the organic fraction of municipal solid waste and fruit and vegetable waste in order to establish the efficiency of a 2 m3 volume pilot plant in terms of biogas and methane yield and stability of the process. The research study presents the feasibility of developing anaerobic digestion as an effective method for municipal solid waste management. The experiments were conducted in mesophilic conditions (35 °C). Domestic waste water was used as inoculum. The results showed that the inoculum presence, temperature, and pH control, were essential in order to improve biogas production and its composition. Using liquid inoculum, the CH4 percentage in the biogas oscillated between 44% and 51%, and the biogas production from 0.504 and 0.6 m3/day. Compared to domestic waste water, animal manure increased the CH4 concentration in biogas (up to 63%), while the daily biogas production increased by 26% and varied from 0.693 to 0.786 m3. The cumulative biogas production at the end of the experiments were 11.7 m3 and 15.89 m3, respectively. Using inoculum and co-digestion, the plant startup time was significantly reduced, the total solids content decreased from 22.7% to 19.8%, while the volatile solids decreased from 37.6% to 31.2%.

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“…Agri-food wastes are additional cheap, sustainable and attractive substrate for the production of biopolymers or other high value-added products [21]. Many studies have assessed the recycling and the potential use of agri-food wastes and byproducts or dedicated energy crops for producing polyhydroxyalkanoate (PHA) [22,23], succinic acid [24][25][26][27], biofuels and biogas [19,[28][29][30][31][32] as well as biological hydrogen and volatile fatty acids [33,34]. Natural environments represent important sources of microbial strains that exert interesting enzymatic activities for biotechnological applications [35].…”

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confidence: 99%

Bioprospecting of exopolysaccharide-producing bacteria from different natural ecosystems for biopolymer synthesis from vinasse

Ventorino

Nicolaus

Donato

et al. 2019

Chem. Biol. Technol. Agric.

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Background: Exopolysaccharides (EPSs) belong to a family of organic thickeners or alternative hydrocolloids of microbial origin. Because the chemical structure offers beneficial bioactive functions, biocompatibility and biodegradability, EPSs are used in the chemical, food, pharmaceutical, cosmetics, and packaging industries as well as in agriculture and medicine. In this study, new bacterial strains were selected on the basis of their ability to synthesize EPS from substrate containing vinasse as a nutrient source to identify the best candidate for bio-based polymer production. Results: Among the 99 newly identified bacterial strains isolated from different natural ecosystem, the strain Azotobacter chroococcum 76A was selected as the best biopolymer producer since it synthesized the highest concentration of EPS in all media containing vinasse. The maximum EPS concentration (44.6 ± 0.63 mg/50 mL) was observed at 24 h, corresponding to its sub-stationary growth phase (7 × 10 8 ± 0.29 CFU/mL). Chemical characterization of the EPS produced showed that carbohydrates representing the principal component, followed by uronic acids and proteins. Interestingly, comparing the IR spectrum of the EPS with alginate by FTIR-ATR analysis revealed an overlap of a peak identified as guluronic acid, a component of alginate. Conclusions: The potential biotechnological capacity of A. chroococcum 76A to synthetize biopolymer from vinasse, inexpensive starting materials, represents a possible alternative to expensive disposal of agri-food waste through its transformation into high value-added products.

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Pre-treatment and inoculum affect the microbial community structure and enhance the biogas reactor performance in a pilot-scale biodigestion of municipal solid waste

Cited by 46 publications

References 70 publications

Effect of inoculum type and organic loading on biogas production of sunflower meal and wheat straw

Effect of inoculum type and organic loading on biogas production of sunflower meal and wheat straw

Pilot-Scale Anaerobic Co-Digestion of the OFMSW: Improving Biogas Production and Startup

Bioprospecting of exopolysaccharide-producing bacteria from different natural ecosystems for biopolymer synthesis from vinasse

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