Pre-transplantation of Bone Marrow Mesenchymal Stem Cells Amplifies the Therapeutic Effect of Ultrasound-Targeted Microbubble Destruction–Mediated Localized Combined Gene Therapy in Post–Myocardial Infarction Heart Failure Rats

Wang, Wei; Tayier, Baihetiya; Guan, Lina; Yan, Fei; Mu, Yuming

doi:10.1016/j.ultrasmedbio.2022.01.004

Cited by 8 publications

(5 citation statements)

References 37 publications

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“…The overexpression of dual gene, especially in the border zone could improve cardiac electromechanical function synergistically. In addition, BMSCs transplantation may play an amplifying effect 12 . The purpose of this study was to optimize the ratio of the two genes for myocardial infarction complications in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…BMSCs were well prepared within our previous experiment 12 under the rules of the Directive 2010/63/EU of the European Parliament. Four weeks old Sprague-Dawley rats at were euthanized with pentobarbital sodium (60 mg/kg body weight, Sigma-Aldrich Inc., St. Louis, MO, USA) via intraperitoneal injection once for BMSCs.…”

Section: Isolation Culture and Identification Of Bmscsmentioning

confidence: 99%

“…The above viruses were biotinylated and had a titer of 1 x 10 9 plaque-forming units (PFU) / mL. The fluorescein isothiocyanate (FITC) labeled SERCA2a-Ad (F/S-Ad) and rhodamine B labeled Cx43-Ad (R/C-Ad) were successfully prepared in our previous experiment 12 according to the method before 13 .…”

Section: Assembling Of Biotinylated Recombinant Adenovirusmentioning

confidence: 99%

“…On the basis of stem cell therapy, gene transfection therapy has become a new therapeutic direction 4,11 . Our previous work had shown that bone marrow stem cells (BMSCs) based co-delivery of SERCA2a and Cx43 is superior to single gene transfection in the treatment of post-MI complications 12 .…”

mentioning

confidence: 99%

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The Optimization in Co-Transfection of SERCA2a And Cx43 Genes for Myocardial Infarction Complications

Wang

Tayier

et al. 2023

Preprint

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As our previous study shown, the therapeutic effect of double genes (SERCA2a and Cx43) on heart failure after myocardial infarction (MI) were better than that of single gene (SERCA2a or Cx43) therapy on the basis of bone marrow stem cells (BMSCs) transplantation. Based on previous research, the aim of this study was to investigate the optimize ratio of co-delivery of SERCA2a and Cx43 genes for MI therapy after biotinylated microbubbles (BMBs) transplantation via ultrasonic-targeted microbubble destruction (UTMD). Forty rats underwent left anterior descending (LAD) ligation and BMSCs injection into the infarct and border zones. Four weeks later, double genes of SERCA2a and Cx43 with different ratios (1:1, 1:2 and 2:1) were co-delivered into the infarcted heart via the UTMD. Cardiac mechano-electrical function was determined at 4-wk after gene delivery, and the hearts of the rats were harvested for measurement of MI size and detection of SERCA2a and Cx43 expression. Q-PCR analysis of the expression of Nkx2.5 and GATA4 in the myocardial infarct zone and measurement of neovascularization in infarcted heart. After comparing the therapeutic effects of different co-gene ratios, the SERCA2a/Cx43-1:2 group shown remarkable cardiac electrical stability and strengthen the role of anti-arrhythmia. In conclusion, the optimum ratio of SERCA2a/Cx43 gene is 1:2, which take advantageous in maintaining cardiac electrophysiological stability.

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Section: Discussionmentioning

confidence: 99%

Section: Isolation Culture and Identification Of Bmscsmentioning

confidence: 99%

Section: Assembling Of Biotinylated Recombinant Adenovirusmentioning

confidence: 99%

mentioning

confidence: 99%

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The Optimization in Co-Transfection of SERCA2a And Cx43 Genes for Myocardial Infarction Complications

Wang

Tayier

et al. 2023

Preprint

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“…More recently, studies are beginning to explore the synergistic effects of gene therapy in conjunction with stem cell therapies. Wang and colleagues 87 demonstrated the simultaneous delivery of two genes: SERCA2a, which regulates cardiac contraction and relaxation by controlling intracellular Ca 2+ handling, and Cx43, well-known to mediate electrical coupling between cardiomyocytes via gap junctional intracellular communication. Dual-gene therapy was performed in a rat model of acute myocardial infarction both with and without pre-transplantation of bone mesenchymal stem cells (BMSCs) 4 weeks post ligation surgery.…”

Section: Applications Of Ultrasound-assisted Gene Delivery To the Heartmentioning

confidence: 99%

Cardiac gene delivery using ultrasound: State of the field

Singh,

Memari,

et al. 2024

Molecular Therapy - Methods & Clinical Development

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Combined Application of Human Amniotic Membrane Mesenchymal Stem Cells and a Modified PGS-co-PCL Film in an Experimental Model of Myocardial Ischemia–Reperfusion Injury

Bahrami

Ale-Ebrahim

Asadi

et al. 2023

Appl Biochem Biotechnol

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Pre-transplantation of Bone Marrow Mesenchymal Stem Cells Amplifies the Therapeutic Effect of Ultrasound-Targeted Microbubble Destruction–Mediated Localized Combined Gene Therapy in Post–Myocardial Infarction Heart Failure Rats

Cited by 8 publications

References 37 publications

The Optimization in Co-Transfection of SERCA2a And Cx43 Genes for Myocardial Infarction Complications

The Optimization in Co-Transfection of SERCA2a And Cx43 Genes for Myocardial Infarction Complications

Cardiac gene delivery using ultrasound: State of the field

Combined Application of Human Amniotic Membrane Mesenchymal Stem Cells and a Modified PGS-co-PCL Film in an Experimental Model of Myocardial Ischemia–Reperfusion Injury

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