Pre-transplant manipulation processing of umbilical cord blood units: Efficacy of Rubinstein’s thawing technique used in 40 transplantation procedures

Zinno, Francesco; Landi, Fabiola; Aureli, Viviana; Caniglia, Maurizio; Pinto, Rita Maria; Rana, Ippolita; Balduino, Geppina; Miele, Massimino Jan; Picardi, Alessandra; Arcese, William; Isacchi, G

doi:10.1016/j.transci.2010.07.005

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…The majority of transplant centers adopted this approach, and nucleated cell count recoveries of 75–90% have been reported. 12,13 More recently, Barker and colleagues described a dilution only “no wash” method with reconstitution in dextran-albumin for a final 5% DMSO concentration. 14 Nucleated cell count recovery was 86%, and there were no serious adverse infusion events.…”

Section: Introductionmentioning

confidence: 99%

Effect of Cord Blood Processing on Transplantation Outcomes after Single Myeloablative Umbilical Cord Blood Transplantation

Ballen

Logan

Laughlin

et al. 2015

Biology of Blood and Marrow Transplantation

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Variations in cord blood manufacturing and administration are common, and the optimal practice, not known. We compared processing and banking practices at 16 public cord blood banks (CBB) in the United States, and assessed transplant outcomes on 530 single umbilical cord blood (UCB) myeloablative transplantations for hematologic malignancies, facilitated by these banks. UCB banking practices were separated into three mutually exclusive groups based on whether processing was automated or manual; units were plasma and red blood cell reduced or buffy coat production method or plasma reduced. Compared to the automated processing system for units, the day-28 neutrophil recovery was significantly lower after transplantation of units that were manually processed and plasma reduced (red cell replete) (odds ratio [OR] 0.19 p=0.001) or plasma and red cell reduced (OR 0.54, p=0.05). Day-100 survival did not differ by CBB. However, day-100 survival was better with units that were thawed with the dextran-albumin wash method compared to the “no wash” or “dilution only” techniques (OR 1.82, p=0.04). In conclusion, CBB processing has no significant effect on early (day 100) survival despite differences in kinetics of neutrophil recovery.

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Section: Introductionmentioning

confidence: 99%

Effect of Cord Blood Processing on Transplantation Outcomes after Single Myeloablative Umbilical Cord Blood Transplantation

Ballen

Logan

Laughlin

et al. 2015

Biology of Blood and Marrow Transplantation

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“…Miura J. et al исследовали 54 образца ПК, подвергшихся криозамораживанию, процент сохранения ЯСК и КОЕ-ГМ в которых составил 96,0±11,3% и 88,6±41,2% соответственно [18]. В исследовании Zinno F. et al процент сохранения ЯСК в 40 образцах ПК после криохранения составил 78,9±15,4%, из них 85,5±10,6% жизнеспособных клеток [19]. Таким образом, анализ способности к колониеобразованию клеток-предшественников гемопоэза в образцах ПК после криохранения подтвердил, что пролиферативная активность ГСК пуповинной крови при использовании нашего протокола замораживания с использованием ДМСО в 6%-ном гидроксиэтилкрахмале остается на высоком уровне.…”

Section: результаты и обсуждениеunclassified

The Amount and Functional Activity of Umbilical Cord Blood Hematopoietic Stem Cells

М.А.¹,

Исайкина²,

Р.Л.³

et al. 2022

Гематология. Трансфузиология. Восточная Европа

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Введение. Пуповинная кровь (ПК) является важным источником гемопоэтических стволовых клеток (ГСК) для пациентов, имеющих широкий спектр онкологических и гематологических заболеваний. Успешное приживление трансплантата пуповинной крови зависит от сбора адекватного количества гемопоэтических клеток с последующей их криоконсервацией без серьезных потерь в количестве и жизнеспособности. Цель. Оценка влияния процессов криозамораживания/оттаивания и хранения при сверхнизкой температуре на содержание и функциональную активность ГСК в образцах ПК. Материалы и методы. Криозамораживание образцов ПК проводили с использованием программируемого замораживателя с добавлением криопротектора с последующим хранением в специализированном криохранилище. Подсчет количества и жизнеспособности ядросодержащих клеток (ЯСК) осуществляли до криозамораживания и после оттаивания. Функциональную активность оценивали по колониеобразующей способности ГСК в среде метилцеллюлозы с добавлением рекомбинантных ростовых факторов. Результаты. Нами не выявлено достоверной разницы в содержании ЯСК до замораживания – 9,9×106 (8,25; 12,23)×106 и после размораживания – 8,66×106 (6,85; 10,79)×106 образцов ПК (p=0,0859). Наблюдалось достоверное снижение жизнеспособности клеток ПК после оттаивания в среднем на 10% (p=0,0001) и после отмывки от криоконсерванта в среднем на 17% (p=0,0001). Было показано отсутствие достоверной разницы в абсолютном количестве колониеобразующих единиц (КОЕ) до и после криозамораживания/оттаивания (p<0,05). Выводы. Процессы криозамораживания/оттаивания и хранения при сверхнизкой температуре не приводят к значительной потере ЯСК в образцах пуповинной крови, но достоверно снижают показатели жизнеспособности. ГСК ПК сохраняют функциональную активность и их пролиферативный потенциал после оттаивания. Introduction. Umbilical cord blood (UCB) is a significant source of hematopoietic stem cells (HSC) for patients with of oncological and hematological diseases. Collection of an adequate number of hematopoietic cells and cryopreservation without serious losses in number and viability can significance for success engraftment of UCB transplant. Purpose. Evaluation of freezing/thawing and storage at ultra-low temperature on the content and functional activity of HSCs in UCB units. Materials and methods. UCB units was cryopreserved with freezing solution by programmer freezer. UCB samples were transferred to specialized cryostorage. The number and viability of nucleated cells (NCs) were counted before freezing and after thawing. Functional activity was assessed by the colony-forming ability of HSCs in methylcellulose medium, with recombinant growth factors. Results. The significant difference in the content of NС in samples before freezing – 9,9×106 (8.25; 12.23)×106 and after thawing – 8,66×106 (6.85; 10.79)×106 (p=0.0859) have been not revealed. Research has been demonstrated a significant decrease in the viability of UCB cells after thawing by an average of 10% (p=0.0001) and after washing by an average of 17% (p=0.0001). No significant difference has been shown in the absolute number of colony forming units (CFU) before and after freezing/thawing (p<0.05). Conclusions. The freezing/thawing and storage at ultra-low temperature maintain amount of NC in cord blood units, but significantly reduce viability. UCB HSCs retain functional activity and proliferative potential after thawing.

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“…In 2007, Woods et al (28) found that adding and removing DMSO at ambient (room) temperature was better. Most groups (30) now cool the blood down to ambient or lower temperatures before adding DMSO to 10% concentration before freezing the cord blood at the rate of −1° per min.…”

Section: Rcr Versus Pd Cord Bloodmentioning

confidence: 99%

Plasma-Depleted versus Red Cell-Reduced Umbilical Cord Blood

Young

2014

Cell Transplant

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Umbilical cord blood banks use two methods to store frozen umbilical cord blood (UCB): red cell reduction (RCR) or plasma depletion (PD). The RCR method centrifuges cord blood in hetastarch or albumin to isolate 21 ml of cord blood containing mostly white blood cells, adds 4 ml of 50% dimethyl sulfoxide (DMSO), and then freezes the resulting 25 ml of cell suspension. The PD method removes plasma, saves all the cells, and freezes the cells in 10% DMSO. PD UCB units are cheaper to process but more expensive to store and somewhat more troublesome to thaw. However, when properly thawed and washed, PD UCB units have as many or more total nucleated cells (TNCs), CD34+ cells, and colony-forming units (CFU) than RCR units. Two studies suggest that PD units have 20-25% more TNCs, MNCs, and CD34 + cells, as well as two to three times more CFU than RCR units. Higher TNC, CD34 + , and CFU counts predict engraftment rate with faster neutrophil and platelet recovery. PD units have high engraftment rates with low mortality and high disease-free survival, comparable with clinical results of treatments with RCR units. One recent series of studies suggests that PD units are more effective for treating thalassemia with 2-year survival rates of 88%, disease-free survival rates of 74%, and 100% cure rate for children under age 7, compared to only 61% overall survival and 23% diseasefree survival rate in thalassemic children treated with RCR units. These findings suggest that PD units not only have more TNCs, CD34 + cells, and CFU than RCR units but also have high engraftment rates and may be more effective for treating certain conditions such as b-thalassemia.

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Pre-transplant manipulation processing of umbilical cord blood units: Efficacy of Rubinstein’s thawing technique used in 40 transplantation procedures

Cited by 5 publications

References 15 publications

Effect of Cord Blood Processing on Transplantation Outcomes after Single Myeloablative Umbilical Cord Blood Transplantation

Effect of Cord Blood Processing on Transplantation Outcomes after Single Myeloablative Umbilical Cord Blood Transplantation

The Amount and Functional Activity of Umbilical Cord Blood Hematopoietic Stem Cells

Plasma-Depleted versus Red Cell-Reduced Umbilical Cord Blood

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