Pre-senescence Harvest of Switchgrass Inhibits Xylose Utilization by Engineered Yeast

Ong, Rebecca G.; Shinde, Somnath; Sousa, Leonardo da Costa; Sanford, Gregg R.

doi:10.3389/fenrg.2018.00052

Cited by 7 publications

(7 citation statements)

References 40 publications

(66 reference statements)

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“…Ash content in switchgrass has been shown to be significantly affected by location and landscape position; for example, high ash content biomass derived from low elevation fields or floodplains may require additional preprocessing to prevent slagging in thermochemical conversion . Previous studies have demonstrated the effects of harvest timing , and harvest equipment and method , on quality variations that impact sugar utilization during fermentation and the quality of convertible biomass for biochemical conversion pathways. The results indicate substantial variability exists regionally, even among counties within close proximity that are representative of a realistic, biorefinery supply shed.…”

Section: Resultsmentioning

confidence: 99%

Multiscale Characterization of Lignocellulosic Biomass Variability and Its Implications to Preprocessing and Conversion: a Case Study for Corn Stover

Ray

Williams

Hoover

et al. 2020

ACS Sustainable Chem. Eng.

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Feedstock variability that originates from biomass production and field conditions propagates through the value chain, posing a significant challenge to the emerging biorefinery industry. Variability in feedstock properties impacts feeding, handling, equipment operations, and conversion performance. Feedstock quality attributes, and their variations, are often overlooked in assessing feedstock value and utilization for conversion to fuels, chemicals, and products. This study developed and employed a multiscale analytical characterization approach coupled with data analytic methods to better understand the sources and distribution of feedstock quality variability through evaluation of 24 corn stover bales collected in 4 counties of Iowa. In total, 216 core samples were generated by sampling nine positions on each bale using a reliable bale coring process. The samples were characterized for a broad suite of physicochemical properties ranging across field and bale, macro, micro, and molecular scales. Results demonstrated that feedstock quality attributes can vary at all spatial scales and that multiple sources of variability must be considered in order to establish and manage biomass quality for conversion processes.

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Section: Resultsmentioning

confidence: 99%

Multiscale Characterization of Lignocellulosic Biomass Variability and Its Implications to Preprocessing and Conversion: a Case Study for Corn Stover

Ray

Williams

Hoover

et al. 2020

ACS Sustainable Chem. Eng.

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“…One of the causes for the high production costs is the low volumetric productivity (Ferreira et al, 2018). Although many previous studies have engineered the microorganisms to improve the fermentation rates of glucose and xylose, the overall xylose fermentation rate remains slow (Ong et al, 2018; Yang S. et al, 2018; Xia et al, 2019). On the other hand, process engineering could be used to enhance the volumetric productivity (Matano et al, 2013; Zhou et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Boosting Ethanol Productivity of Zymomonas mobilis 8b in Enzymatic Hydrolysate of Dilute Acid and Ammonia Pretreated Corn Stover Through Medium Optimization, High Cell Density Fermentation and Cell Recycling

Li¹,

Zhai²,

Jiang³

et al. 2019

Front. Microbiol.

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The presence of toxic degradation products in lignocellulosic hydrolysate typically reduced fermentation rates and xylose consumption rate, resulting in a decreased ethanol productivity. In the present study, Zymomonas mobilis 8b was investigated for high cell density fermentation with cell recycling to improve the ethanol productivity in lignocellulosic hydrolysate. The fermentation performances of Z. mobilis 8b at various conditions were first studied in yeast extract-tryptone medium. It was found that nutrient level was essential for glucose and xylose co-fermentation by Z. mobilis 8b and high cell density fermentation with cell recycling worked well in yeast extract-tryptone medium for 6 rounds fermentation. Z. mobilis 8b was then studied in enzymatic hydrolysates derived from dilute acid (DA) pretreated corn stover (CS) and ammonia pretreated CS for high cell density fermentation with cell recycling. Ethanol productivity obtained was around three times higher compared to traditional fermentation. Ethanol titer and metabolic yield were also enhanced with high cell density fermentation. Z. mobilis 8b cells showed high recyclability in ammonia pretreated CS hydrolysate.

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“…While this suggestion is at odds with the idea that wild viruses typically are not pathogenic in their natural hosts, it is supported by the frequent expression of symptoms in infected switchgrass. The extent to which premature senescence might translate to reduced bioenergy production depends on the interplay among its effects on biomass yield, nutrient resorption, and conversion efficiencies (Ong et al, 2018). While any optimization of harvest timing can be complex and specific to the conversion process (Ong et al, 2018), premature senescence shortens the growing period and is likely to reduce yield potential in all cases.…”

Section: Impact On Switchgrassmentioning

confidence: 99%

“…The extent to which premature senescence might translate to reduced bioenergy production depends on the interplay among its effects on biomass yield, nutrient resorption, and conversion efficiencies (Ong et al, 2018). While any optimization of harvest timing can be complex and specific to the conversion process (Ong et al, 2018), premature senescence shortens the growing period and is likely to reduce yield potential in all cases. Moreover, if premature senescence extends the time between senescence and harvest date, it provides additional opportunities for dry biomass to be lost to wind or drop to the ground beyond the reach of harvesting equipment (Adler et al, 2006;Anderson et al, 2013).…”

Section: Impact On Switchgrassmentioning

confidence: 99%

Emerging wild virus of native grass bioenergy feedstock is well‐established in the Midwestern USA and associated with premature stand senescence

et al. 2022

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The North American native prairie grass Panicum virgatum (switchgrass) is a primary bioenergy feedstock candidate. Its widespread distribution and genetic diversity enable the possibility of developing this perennial grass for high production in a variety of conditions, including on marginal lands. A critical concern in feedstock development and deployment is the risk of novel pathogen emergence.Here, we investigate the landscape-scale prevalence and epidemiology of a littlestudied North American virus that was first detected in switchgrass and other grasses in bioenergy trials in the US Midwest. Switchgrass mosaic virus (SwMV, Genus Marafivirus, Family Tymoviridae) is transmitted by leafhoppers and is phylogenetically a sister to Maize rayado fino virus, a significant pathogen of maize in parts of the Americas. Our goal was to determine whether SwMV is uniquely limited to specific bioenergy trials or well-established and circulating more broadly. We used molecular diagnostics to quantify naturally occurring SwMV infection in leafhoppers and switchgrass in naturalistic stands throughout a large Midwestern landscape, and quantified leafhopper abundances and stand performance. Our analysis revealed that this apparently wild virus is well-established and widespread. Infection was present at nearly all sites, across diverse landscape contexts, with its prevalence ranging as high as 33%-60%. Infection appeared to

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Pre-senescence Harvest of Switchgrass Inhibits Xylose Utilization by Engineered Yeast

Cited by 7 publications

References 40 publications

Multiscale Characterization of Lignocellulosic Biomass Variability and Its Implications to Preprocessing and Conversion: a Case Study for Corn Stover

Multiscale Characterization of Lignocellulosic Biomass Variability and Its Implications to Preprocessing and Conversion: a Case Study for Corn Stover

Boosting Ethanol Productivity of Zymomonas mobilis 8b in Enzymatic Hydrolysate of Dilute Acid and Ammonia Pretreated Corn Stover Through Medium Optimization, High Cell Density Fermentation and Cell Recycling

Emerging wild virus of native grass bioenergy feedstock is well‐established in the Midwestern USA and associated with premature stand senescence

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