Pre-programmed microdroplet generator to control wide-ranging chemical concentrations

Ahamed, Md. Ahasan; Kim, Geunyong; Li, Zhenglin; Kim, Sung Jin

doi:10.1016/j.aca.2022.340587

Cited by 7 publications

(5 citation statements)

References 21 publications

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“…Fluorescent signal devices are more convenient and faster compared to the SERS and super-resolution microscopy, making it more suitable for application in POCT. Zhou for the simultaneous detection of multiple miRNA by employing multiple donor and acceptor pairs (Ahamed et al, 2022;Qiu & Hildebrandt, 2015;Thapa et al, 2022). Chen et al devised a novel assay for the simultaneous detection of multiple miRNA, employing DNA tetrahedron nanotags coupled with FRET between the nucleic acid stain TOTO-1 and three different organic dyes (Figure 4b) (Zhao, Wang, Xiong, et al, 2020).…”

Section: Multiple Mirna Hybridizationmentioning

confidence: 99%

“…This fluorescent sensor platform is designed to detect multiple types of miRNA simultaneously within the same system, offering high specificity and minimal cross‐reactivity. Meanwhile, the fluorescent resonance energy transfer (FRET)‐based multiplexed technique has become a powerful tool for the simultaneous detection of multiple miRNA by employing multiple donor and acceptor pairs (Ahamed et al, 2022; Qiu & Hildebrandt, 2015; Thapa et al, 2022). Chen et al devised a novel assay for the simultaneous detection of multiple miRNA, employing DNA tetrahedron nanotags coupled with FRET between the nucleic acid stain TOTO‐1 and three different organic dyes (Figure 4b) (Zhao, Wang, Xiong, et al, 2020).…”

Section: Extraction and Detection Of Salivary Mirna At Poctmentioning

confidence: 99%

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Sample‐to‐answer salivary miRNA testing: New frontiers in point‐of‐care diagnostic technologies

Zhang,

Liu,

Dong

et al. 2024

WIREs Nanomed Nanobiotechnol

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MicroRNA (miRNA), crucial non‐coding RNAs, have emerged as key biomarkers in molecular diagnostics, prognosis, and personalized medicine due to their significant role in gene expression regulation. Salivary miRNA, in particular, stands out for its non‐invasive collection method and ease of accessibility, offering promising avenues for the development of point‐of‐care diagnostics for a spectrum of diseases, including cancer, neurodegenerative disorders, and infectious diseases. Such development promises rapid and precise diagnosis, enabling timely treatment. Despite significant advancements in salivary miRNA‐based testing, challenges persist in the quantification, multiplexing, sensitivity, and specificity, particularly for miRNA at low concentrations in complex biological mixtures. This work delves into these challenges, focusing on the development and application of salivary miRNA tests for point‐of‐care use. We explore the biogenesis of salivary miRNA and analyze their quantitative expression and their disease relevance in cancer, infection, and neurodegenerative disorders. We also examined recent progress in miRNA extraction, amplification, and multiplexed detection methods. This study offers a comprehensive view of the development of salivary miRNA‐based point‐of‐care testing (POCT). Its successful advancement could revolutionize the early detection, monitoring, and management of various conditions, enhancing healthcare outcomes.This article is categorized under: Diagnostic Tools > Biosensing Diagnostic Tools > Diagnostic Nanodevices

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Section: Multiple Mirna Hybridizationmentioning

confidence: 99%

Section: Extraction and Detection Of Salivary Mirna At Poctmentioning

confidence: 99%

Sample‐to‐answer salivary miRNA testing: New frontiers in point‐of‐care diagnostic technologies

Zhang,

Liu,

Dong

et al. 2024

WIREs Nanomed Nanobiotechnol

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“…62 Prior studies have employed micro and milli-fluidic platforms for live animals, [63][64][65] including zebrafish, [66][67][68] and various respirometry techniques have measured oxygen consumption in marine species exposed to contaminants. [69][70][71] This microfluidic system, compact and capable of temperature control, minimizes sensor noise [72][73][74][75][76][77] and provides consistent hatching conditions. In a previous study by our group, 62 a similar setup was used to evaluate optimal hatching conditions for Artemia, where it was observed that the hatching stage could be identified through metabolic rates and morphology and both the temperature and salinity affected the duration of hatching stages, metabolic rates, and hatchability.…”

Section: Introductionmentioning

confidence: 99%

Real-time assessment of the impacts of polystyrene and silver nanoparticles on the hatching process and early-stage development of Artemia using a microfluidic platform

Dey,

Bradley,

Boymelgreen

2024

Environ. Sci.: Nano

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“…The proposed method enables the fabrication of channels with rectangular and round cross sections in one device in a facile manner. Our pressure regulator system is envisioned for application in constant-pressure sources for integrated microfluidic systems employed in blood plasma separation [24], cell culturing [25,26], droplet generation [27], and layer-by-layer assembly [28].…”

Section: Introductionmentioning

confidence: 99%

Method to fabricate round cross‐sectional channel using thermal expansion of air for passive flow regulators

Kabir,

Kim

2023

Electrophoresis

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We present a novel method for fabricating round cross‐sectional channels for autoregulatory pressure regulators. The application of previously reported methods in multilayered soft lithography using three‐dimensional printed molds is challenging. Herein, we used a thermal expansion technique to create round cross‐sectional channels in replica layers using air in the cavity space of a master mold. The width and height of the round channel in the replica could be adjusted in the range of 80–300 and 3–57 µm, respectively, by varying the precuring time of the replica in the gel state and adjusting the cavity size of the master mold. We successfully fabricated a pressure regulator with a round cross‐sectional channel, achieving a constant output pressure at a low threshold input pressure. Our device exhibited superior performance, with a constant output pressure at a threshold input pressure of less than 77%, compared to a device with a rectangular cross‐sectional channel. Our method has significant potential for application in the fabrication of integrated microfluidic systems with round cross‐sectional channels.

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Pre-programmed microdroplet generator to control wide-ranging chemical concentrations

Cited by 7 publications

References 21 publications

Sample‐to‐answer salivary miRNA testing: New frontiers in point‐of‐care diagnostic technologies

Sample‐to‐answer salivary miRNA testing: New frontiers in point‐of‐care diagnostic technologies

Real-time assessment of the impacts of polystyrene and silver nanoparticles on the hatching process and early-stage development of Artemia using a microfluidic platform

Method to fabricate round cross‐sectional channel using thermal expansion of air for passive flow regulators

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