Pre-operative anesthetic assessment of patients with rheumatoid arthritis

Aires, Rodrigo Barbosa; Carvalho, Jozélio Freire de; Mota, Lícia Maria Henrique da

doi:10.1016/j.rbre.2013.08.002

Cited by 8 publications

(8 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TXNIP has been found to be involved in some ion channel regulations [39] . Regarding the clinical treatment of rheumatoid arthritis, many anesthetics have been applied in therapy to relieve rheumatoid arthritis pain [40,41] . These drugs usually have multiple ion channel targets [42,43] .…”

Section: Discussionmentioning

confidence: 99%

The CDKN2B-AS1/ MIR497/TXNIP axis regulated macrophages

Liu

et al. 2021

Preprint

View full text Add to dashboard Cite

The polarization of macrophages plays a critical role in the pathophysiology of rheumatoid arthritis. The polarization states include pro-inflammatory M1 polarization and various alternative anti-inflammatory M2 polarization. Our preliminary results showed that CDKN2B-AS1/MIR497/TXNIP axis might play a role in macrophages extracted from rheumatoid arthritis patients. Therefore, we hypothesized that this axis regulated the polarization of rheumatoid macrophages. Flow cytometry was used to determine the surface polarization markers in M1 or M2 macrophages from healthy donors and rheumatoid arthritis patients. QPCR and western blotting were used to compare the expression of the CDKN2B-AS1/MIR497/TXNIP axis in these macrophages. We interfered with the expression and function of the axis from upstream to downstream in the macrophage cell line MD to test its roles in macrophage polarization. Compared to cells from healthy donors, cells from rheumatoid arthritis patients expressed a higher level of CD40 and CD80 and a lower level of CD16, CD163, CD206, and CD200R after polarization, they also expressed higher CDKN2B-AS1, lower MIR497, and higher TXNIP. In macrophages from healthy donors, there was no correlation among CDKN2B-AS1, MIR497, and TXNIP. But in macrophages patients, they showed significant correlations. The CDKN2B-AS1 knockdown, MIR497 mimics suppressed M1 polarization but promoted M2 polarization in MD cells, while MIR497 knockdown and TXNIP overexpression did the opposite. This study demonstrated that elevated CDKN2B-AS1 in macrophages promotes M1 polarization and inhibits M2 polarization of macrophage by negatively regulating MIR497, thereby upregulated the expression of TXNIP.

show abstract

Section: Discussionmentioning

confidence: 99%

The CDKN2B-AS1/ MIR497/TXNIP axis regulated macrophages

Liu

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…TXNIP has been found to be involved in some ion channel regulations [39] . Furthermore, regarding the clinical treatment of rheumatoid arthritis, many anesthetics have been applied in therapy to relieve rheumatoid arthritis pain [40,41] . These drugs usually have multiple ion channel targets [42,43] .…”

Section: Discussionmentioning

confidence: 99%

Version up-date: The CDKN2B-AS1/ MIR497/TXNIP axis regulated macrophages

2021

Preprint

View full text Add to dashboard Cite

The polarization of macrophages plays a critical role in the pathophysiology of rheumatoid arthritis. The macrophages can have pro-inflammatory M1 polarization and various types of alternative anti-inflammatory M2 polarization. Our preliminary results showed that the CDKN2B-AS1/MIR497/TXNIP axis might regulate macrophages of rheumatoid arthritis patients. Therefore, we hypothesized that this axis regulated the polarization of rheumatoid macrophages. Flow cytometry was used to determine the surface polarization markers in M1 or M2 macrophages from healthy donors and rheumatoid arthritis patients. The QPCR and Western Blotting were used to compare the expression of the CDKN2B-AS1/MIR497/TXNIP axis in these macrophages. We Knocked down and overexpressed the axis in the macrophage cell line MD to test its roles in macrophage polarization. Compared to cells from healthy donors, cells from rheumatoid arthritis patients expressed higher levels of CD40 and CD80 and lower levels of CD16, CD163, CD206, and CD200R after polarization, they also expressed higher CDKN2B-AS1, lower MIR497, and higher TXNIP. In macrophages from healthy donors, there was no correlation among CDKN2B-AS1, MIR497, and TXNIP. But in macrophages from patients, there were significant correlations. The CDKN2B-AS1 knockdown, MIR497 mimics suppressed the M1 polarization but promoted the M2 polarization in MD cells, while the MIR497 knockdown and the TXNIP overexpression did the opposite. This study demonstrated that elevated CDKN2B-AS1 in macrophages promotes the M1 polarization and inhibited the M2 polarization of macrophages by the CDKN2B-AS1/ MIR497/TXNIP axis.

show abstract

“…This needs to be taken into consideration, especially if general anaesthesia is to be used. In these patients, it is recommended that these patients are to be intubated with fibre optic laryngoscopes and that hyperextension of the neck should be prevented (Aires et al 2014).…”

Section: Intraoperative Managementmentioning

confidence: 99%

Perioperative management of patients with rheumatoid arthritis undergoing orthopaedic surgery

Agarwal

Kamaraj

McDonnell

et al. 2019

Journal of Perioperative Practice

View full text Add to dashboard Cite

Rheumatoid arthritis is an autoimmune disease which is characterised by systemic inflammation. In the recent decades, the advent of disease modifying anti-rheumatic drugs have reduced the need for orthopaedic procedures. However, there are numerous patients in which pharmacological treatment fails and these patients require surgical intervention. Complications can arise due to the underlying pathology and multitude of medications these patients can be on, specifically increased infection rates due to use of immunosuppressive agents. An understanding into the disease itself, as well as perioperative management, may improve overall patient outcomes and prevent future complications.

show abstract

Pre-operative anesthetic assessment of patients with rheumatoid arthritis

Cited by 8 publications

References 35 publications

The CDKN2B-AS1/ MIR497/TXNIP axis regulated macrophages

The CDKN2B-AS1/ MIR497/TXNIP axis regulated macrophages

Version up-date: The CDKN2B-AS1/ MIR497/TXNIP axis regulated macrophages

Perioperative management of patients with rheumatoid arthritis undergoing orthopaedic surgery

Contact Info

Product

Resources

About