Pre-miRNA expressing plasmid delivery for anti-cancer therapy

Benisvy-Aharonovich, Elena; Shimanovich, Ulyana; Kronfeld, Noam; Giladi, Nis; Bier, Ariel; Kazimirsky, Gila; Gedanken, Aharon; Brodie, Chaya

doi:10.1039/c3md00314k

Cited by 4 publications

(4 citation statements)

References 32 publications

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“…On the other hand, different metallic nanoparticles revealed the ability for miRNA delivery into the brain tumor cells, as for example gold (Au) nanoparticles or superparamagnetic iron oxide nanoparticles (SPIONs) and zinc-doped iron oxide nanoparticles (ZnFe2O4) as magneto-based transfection gene carriers (Kouri et al, 2015, Lo et al, 2015, Yin et al, 2014, Benisvy-Aharonovich et al, 2014. Chondroitin sulfatepolyethylenimine (CS-PEI) copolymer-coated poly(acrylic acid)-bound iron oxide (PAAIO) nanoparticle (CPIO) complexed to miR-128 expressing pDNA demonstrated an enhanced miR-128 in vitro transfection and downregulation of target genes and improved tumor accumulation in U-87 MG subcutaneous tumor-bearing mice, under the concomitant application of an external magnetic field.…”

Section: Mirna Delivery Nanosystemsmentioning

confidence: 99%

Tailoring drug and gene codelivery nanosystems for glioblastoma treatment

Silva

Mendes

Sousa

et al. 2020

Advances and Avenues in the Development of Novel Carriers for Bioactives and Biological Agents

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Glioblastoma is the most common primary and aggressive brain tumour, with an increasing incidence worldwide. The prognosis of this disease is still poor, with a median survival time not exceeding two years. Standard-of-care therapy includes surgical resection, radio-and chemotherapy, but nearly all patients experience progression of the disease. This may be ascribed to the heterogeneity, invasiveness and resistance of tumour cells, along with the struggle that many chemical drugs present in effectively crossing the dual blood brain-blood brain tumour barrier.Considering the hurdles associated to traditional therapeutic approaches, there is a pressing need to improve patient care, as treatments currently available have little effect on the overall survival. Therefore, the use of adjuvant chemotherapeutics in combination with temozolomide, a first-line drug, and novel molecularly-targeted approaches against both tumor and stem cells and respective microenvironment are under investigation. This chapter addresses the development of innovative multi-target nanomedicines, comprising complementary chemo-(e.g. temozolomide) and gene therapeutic (antimiR and miRNA mimic) agents, combined with targeting ligands within a single nanostructure directed at the treatment of glioblastoma multiforme. The approach aims at providing significantly improved therapeutics, as treatments currently available have little effect on overall survival.

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Section: Mirna Delivery Nanosystemsmentioning

confidence: 99%

Tailoring drug and gene codelivery nanosystems for glioblastoma treatment

Silva

Mendes

Sousa

et al. 2020

Advances and Avenues in the Development of Novel Carriers for Bioactives and Biological Agents

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“…[1][2][3][4] Cationic polymers 5,6 and nanoparticles 7,8 have shown great potential as gene and drug carriers. [1][2][3][4] Cationic polymers 5,6 and nanoparticles 7,8 have shown great potential as gene and drug carriers.…”

Section: Introductionmentioning

confidence: 99%

“…The development of safe and efficient gene carriers is very important for gene therapy. [1][2][3][4] Cationic polymers 5,6 and nanoparticles 7,8 have shown great potential as gene and drug carriers. Cationic polymers, as potential alternatives to viral vectors, show low host immunogenicity and can be easily produced in massive quantities.…”

Section: Introductionmentioning

confidence: 99%

A series of new supramolecular polycations for effective gene transfection

Song

et al. 2015

Polym. Chem.

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The development of safe and effective supramolecular polycations has attracted much attention. In this work, a series of novel cyclodextrin (CD)-based supramolecular assemblies were readily prepared via the host-guest interaction by assembling different adamantane-functionalized α-CD derivatives with multiple β-CD-cored polycations. The supramolecular assemblies were investigated in terms of their pDNA-binding capabilities, cytotoxicities, cellular internalization and gene transfection efficiencies in HEK293, HepG2 and C6 cell lines. The supramolecular polycations displayed low cytotoxicities, similar to those of the CD-cored carriers.After self-assemblies, the gene transfection efficiencies and antitumor abilities of the supramolecular carriers were significantly enhanced. The present study demonstrates that such supramolecular preparation of CD-based polycations could provide a flexible strategy for the design and development of new assemblies with high efficiency and low cytotoxicity.

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“…Using the sonication technique, several organic nanomaterials were synthesized, 12 such as enzyme (amylase) NPs, 13,14 tannic acid NPs, or even RNA NPs. 15,16 This study focuses on two organic materials, Penicillin and Vitamin B 12 . In addition to these two organic materials, other materials like tannic acid and more, [12][13][14][15][16][17] were synthesized in a similar way by us and others.…”

mentioning

confidence: 99%

Enhanced pharmacological activity of Vitamin B12 and Penicillin as nanoparticles

Yariv

Lipovsky

Gedanken

et al. 2015

IJN

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Sonochemistry has become a well-known technique for fabricating nanomaterials. Since one of the advantages of nanomaterials is that they have higher chemical activities compared with particles in the bulk form, efforts are being made to produce nano organic compounds with enhanced biological activities that could be exploited in the medical area. This study uses the sonication technique to prepare nano Vitamin B 12 and nano Penicillin, and demonstrates their enhanced biological and pharmacological activity. The size and morphology of the nano Penicillin and nano Vitamin B 12 were investigated using electron microscopy as well as dynamic light scattering techniques. The sizes of Penicillin and Vitamin B 12 nanoparticles (NPs) were found to be 70 and 120–180 nm, respectively. The bactericidal effect of nano Penicillin was studied and found to be higher than that of the bulk form. Reducing the size of Vitamin B 12 resulted in their enhanced antioxidative activity as observed using the electron paramagnetic spectroscopy technique. The penetration depth of these organic NPs can be detected by an optical iterative method. It is believed that nano organic drugs fabrication will have a great impact on the medical field.

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Pre-miRNA expressing plasmid delivery for anti-cancer therapy

Abstract: The premiR145/GFP expressing plasmid DNA was delivered into glioma cells and the transcripted miRNA145 efficiently decreases the expression of CTGF.

Cited by 4 publications

References 32 publications

Tailoring drug and gene codelivery nanosystems for glioblastoma treatment

Tailoring drug and gene codelivery nanosystems for glioblastoma treatment

A series of new supramolecular polycations for effective gene transfection

Enhanced pharmacological activity of Vitamin B12 and Penicillin as nanoparticles

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