Pre-induction of Hsp70 expression to protect the grooved carpet shell clam, Ruditapes decussatus, against Micrococcus luteus: A trained immunity strategy

El-Wazzan, Eman; Ghareeb, Doaa A.; Abdella, Bahaa

doi:10.1016/j.ejar.2019.10.004

Cited by 5 publications

(2 citation statements)

References 26 publications

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“…These fish are prime candidates for aquaculture and have been widely cultivated [12,13]. However, it's important to note that freshwater bodies, their primary habitat, also host the zoonotic pathogen Aeromonas hydrophila, which can infect fish, bivalves, amphibians, reptiles, and humans [14][15][16]. Two successful candidates in freshwater aquaculture are Sperata sarwari and Channa marulius, cultivated commercially in various regions, including Pakistan, India, Bangladesh, China, and Indonesia, where they are top producers [17].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Aeromonas hydrophila detected in Channa marulius and Sperata sarwari sampled from rivers of Punjab in Pakistan

Mahmood,

Rasool,

Hafeez-ur-Rehman

et al. 2024

PLoS ONE

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Aeromonas hydrophila is one of the major pathogenic bacteria responsible for causing severe outbreaks at fish farms and is also a major global public health concern. This bacterium harbors many virulence genes. The current study was designed to evaluate the antidrug and virulence potential of A. hydrophila by amplifying its antimicrobial resistance and virulence genes using PCR and examining their effects on fish tissues and organs. A total of 960 fish samples of Channa marulius and Sperata sarwari were collected from four sites of the rivers of the Punjab, Pakistan. A. hydrophila isolates were subjected to biochemical identification and detection of virulence and antimicrobial resistance (AMR) genes by PCR. We retrieved 181 (6.46%) A. hydrophila isolates from C. marulius and 177 (6.25%) isolates from S. sarwari. Amplification through PCR revealed the incidence of virulence genes in 95.7% of isolates in C. marulius and 94.4% in S. sarwari. Similarly, amplification through PCR also revealed occurrence of AMR genes in 87.1% of isolates in C. marulius and 83.9% in S. sarwari. Histopathological examination revealed congestion (5.2%) and hepatocyte necrosis (4.6%) in liver, lamellar fusion (3.3%) and the presence of bacterial colonies (3.7%) in gills, fin erosion (6%), and the presence of biofilms (3.5%) in tail fins of infected fish. Phylogenetic tree analysis of 16S rRNA and gyrB gene of A. hydrophila revealed 100% and 97% similarity, respectively, with 16S rRNA gene and gyrB of A. hydrophila isolated in previous studies. The results of antimicrobial susceptibility testing showed that all isolates demonstrated resistance to sulfamethoxazole, ampicillin, neomycin, and norfloxacin, while susceptibility to gentamicin, chloramphenicol, and tetracycline, and intermediate resistance was observed against cefotaxime. The results concluded that examined fish samples were markedly contaminated with virulent and multidrug strains of A. hydrophila which may be of a potential health risk. The study emphasizes the responsible antimicrobial use in aquaculture and the urgent need for effective strategies to control the spread of virulence and antimicrobial resistance genes in A. hydrophila.

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Section: Introductionmentioning

confidence: 99%

Molecular characterization of Aeromonas hydrophila detected in Channa marulius and Sperata sarwari sampled from rivers of Punjab in Pakistan

Mahmood,

Rasool,

Hafeez-ur-Rehman

et al. 2024

PLoS ONE

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“…In other words, the presence of such EPS guards against environmental changes such as temperature and salinity fluctuations, as well as possible predators (Nichols et al 2005;Squillaci et al 2016;Rana and Upadhyay 2020) said that the functions of exopolysaccharides depend on the structure of the microorganism and where it lives. Capsular polysaccharides also contributes to vilrulance in pathogenic strains (Deng et al 2010;Abdella et al 2017;El-Wazzan et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Exopolysaccharide production from Alkalibacillus sp. w3: statistical optimization and biological activity

et al. 2022

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Microbial exopolysaccharides (EPS) provide a broad range of applications. Thus, there is an increasing interest in the production, characterization, and use of EPS derived from various microorganisms. Extremophile polysaccharides have unique properties and applications due to its unique structures. The importance of exopolysaccharides synthesized by a new bacterial strain, Alkalibacillus sp. w3, was highlighted in this study. Alkalibacillus sp. w3, a haloalkalitolerant firmicute that was recovered from a salt lake, was optimized for EPS production, and its biological activities were studied. Exopolysaccharide synthesis was observed in Horikoshi I broth medium. The optimal culture conditions for achieving the highest exopolysaccharide production were a 7-day incubation period, pH 10, and 250 g/L of NaCl. The most effective carbon and nitrogen sources for EPS production were glucose and a combination of yeast extract and peptone. Additionally, Plackett-statistical Burman’s design showed that all factors tested had a favorable impact, with glucose having the greatest significance on the production of EPS. The model’s best predictions for culture conditions resulted in a two-fold improvement in EPS production compared to the original yield before optimization. The recovered EPS contained 65.13% carbohydrates, 30.89% proteins, and 3.98% lipids. Moreover, EPS produced by Alkalibacillus sp. w3 demonstrated anticancer activity against hepatocellular carcinoma (HepG2) and human colon carcinoma (HCT-116) cell lines, with IC50 values as low as 11.8 and 15.5 µg/mL, respectively, besides antibacterial activity against various Gram-positive, Gram-negative bacteria, and yeast. Based on these results, EPS made by Alkalibacillus sp. w3 has many useful properties, which make it suitable for use in the medical field.

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Sensitivity of the grooved carpet shell clam, Ruditapes decussatus (Linnaeus, 1758), to ocean acidification

et al. 2023

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Pre-induction of Hsp70 expression to protect the grooved carpet shell clam, Ruditapes decussatus, against Micrococcus luteus: A trained immunity strategy

Cited by 5 publications

References 26 publications

Molecular characterization of Aeromonas hydrophila detected in Channa marulius and Sperata sarwari sampled from rivers of Punjab in Pakistan

Molecular characterization of Aeromonas hydrophila detected in Channa marulius and Sperata sarwari sampled from rivers of Punjab in Pakistan

Exopolysaccharide production from Alkalibacillus sp. w3: statistical optimization and biological activity

Sensitivity of the grooved carpet shell clam, Ruditapes decussatus (Linnaeus, 1758), to ocean acidification

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