Pre-implantation multiple cytokine mRNA expression analysis in donor lung grafts predicts survival after lung transplantation in humans

Increased levels of interleukin-1β and tumor necrosis factor-α in donor lungs rejected for transplantation

“…10,11 Our group used multicytokine analysis of the donor lung graft with qRT-PCR to demonstrate that increased levels of IL-6, IL-8, TNF-␣, and IL-1␤ in accepted donor lungs are risk factors for PGD, whereas IL-10 and IFN-␥ are protective. 7 Indeed, in the same study, we observed that the ratio of pro-inflammatory to anti-inflammatory cytokines (IL-6/IL-10) significantly predicted recipient early death after lung transplantation.…”

“…7,8 The inaccuracy of clinical parameters in determining post-transplant outcomes occasionally leads to the use of lungs with unrecognized injuries, leading to severe PGD. Furthermore, an estimated 40% of the lungs that are currently clinically rejected for transplantation could have been safely used if a more detailed evaluation of the organ was possible.…”

Donor cause of brain death and related time intervals: does it affect outcome after lung transplantation?☆☆☆

“…In a study by Kaneda et al, messenger RNA (mRNA) levels of the cytokines interleukin (IL)-6, IL-8, tumor necrosis factor (TNF)-alpha and IL-1 beta measured in lung biopsies were found to be risk factors for early mortality, while IL-10 and interferon (IFN)-gamma were protective factors [17]. It would be interesting to measure these cytokine levels in bronchioloalveolar lavage fluid in donor lungs at different intervals after brain death in a prospective cohort of donors to see the differences over time elapsed from brain insult until organ recovery and the relation with survival.…”

Initial Experience With Lung Donation After Cardiocirculatory Death in Canada

“…To that end, we and others have demonstrated that elevated levels of the proinflammatory cytokines IL-6, IL-8, and IL-1␤, and low levels of IL-10 in the donor lung tissue can accurately predict increased 30-day mortality due to primary graft dysfunction after LTx in humans. [37][38][39] Interestingly, some preliminary clinical studies have shown that inflammatory profiles are favorable in lungs from DCDs compared with brain-dead donors 40 ; thus, avoidance of the cytokine storm associated with brain death might be an advantage of the DCD lungs.…”