Pre-implantation genetic diagnosis and pre-implantation genetic screening: two years experience at a single center

Abstract

ObjectiveIndications for preimplantation genetic diagnosis (PGD)/preimplantation genetic screening (PGS) cycles and clinical outcomes were evaluated at CHA Gangnam Medical Center.MethodsThis is retrospective cohort study. All patients (n=336) who went through in vitro fertilization (IVF)-PGD/PGS cycles (n=486) between January 2014 and December 2015 were included in Fertility Center of CHA Gangnam Medical Center. Patients underwent IVF-PGD/PGS with 24-chromosome screening. Patients with euploid embryos had transfer of one or 2 embryos in a fresh cycle with any subsequent frozen embryo transfer (ET) cycle. Compared implantation, clinical pregnancy, ongoing pregnancy, and early abortion rates were the main outcome measures.ResultsThe most common indication for PGD/PGS was recurrent spontaneous abortion (n=160). The chromosome rearrangement cases (n=116) included 24 Robertsonian translocations, 60 reciprocal translocations, 3 inversions, 2 deletions, 4 additions, and 23 mosaicisms. PGS cases rather than the PGD cases showed higher implantation rates (26.4% vs. 20.3%), ongoing pregnancy rates (19.5% vs. 16.4%), and clinical pregnancy rates (28.6% vs. 23.3%). Implantation rates (30.3% vs. 23.7%), clinical pregnancy rates (39.2% vs. 25.2%), and ongoing pregnancy rates (25.7% vs. 17.5%) were significant higher in the blastocyst evaluation group than cleavage stage evaluation group.ConclusionThis was the largest study of PGD/PGS for 2 years at a single center in Korea. The pregnancy outcomes of PGD cases are slightly lower than PGS cases. It was confirmed again that success rate of PGD/PGS is higher if biopsy was done at blastocyst than cleavage stage.

Analysis of PGT‐M and PGT‐SR outcomes at a Canadian fertility clinic

“…This study represents the largest PGT‐M and PGT‐SR data set reviewing embryo outcomes and pregnancy rates published in North America. Three other studies have analyzed PGT‐M and/or PGT‐SR outcomes at their centers . A recent study in London reviewed 82 couples undergoing PGT for autosomal and sex‐linked conditions and one case of a single gene disorder and chromosome rearrangement .…”

“…In another study, Won et al reviewed 116 PGT‐SR cycles for 76 patients at a center in Korea. These authors reported 24 Robertsonian translocations, 60 reciprocal translocations, three inversions, two deletions, four additions, and 23 mosaicisms.…”

“…All procedures followed were in accordance with the ethical standards of the responsible committee on human experimentation (institutional and national) and with the Helsinki Declaration of 1975, as revised in 2000 (5). Ethics approval was obtained through the UBC Clinical Research Ethics Board.…”

Pericentric inversion of chromosome 6 and male fertility problems

“…Moreover, these carriers can opt for natural pregnancy along with prenatal diagnosis [ 10 ]. For inversion carriers with asthenospermia, PGD treatment should be recommended [ 19 ]. For inversion carriers with azoospermia, the possible etiology needs further analysis.…”