Pre-harvest hormetic doses of UV-C radiation can decrease susceptibility of lettuce leaves ( Lactuca sativa L.) to Botrytis cinerea L.

Vàsquez, Hilariòn; Ouhibi, Chayma; Lizzi, Yves; Azzouz, Nassera; Forges, Marine; Bardin, Marc; Nicot, Philippe C.; Urban, Laurent; Aarrouf, Jawad

doi:10.1016/j.scienta.2017.04.017

Cited by 45 publications

(17 citation statements)

References 56 publications

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“…Results obtained as part of this study confirm previous observations showing that UV-C light, either under the form of exposures of 60 s or of 1s (Fig 4), can stimulate plant defences [6][7][8][9][10][11]. See also Terry and Joyce for older references [17].…”

Section: Uv-c Light Stimulates Plant Defencessupporting

confidence: 88%

“…UV-B light is known to act through signalling pathways, involving notably mitogen-activated protein kinases, which closely resemble those for pathogens [5]. It has also been observed that UV-C light can stimulate plant and crop defences against Botrytis cinerea and Sclerotinia minor [6][7][8][9][10][11]. UV-B light requires extensive periods of irradiation (several hours or days) to be effective.…”

Section: Introductionmentioning

confidence: 99%

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Flashes of UV-C light: An innovative method for stimulating plant defences

Aarrouf

Urban

2020

PLoS ONE

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Leaves of lettuce, pepper, tomato and grapevine plants grown in greenhouse conditions were exposed to UV-C light for either 60 s or 1 s, using a specific LEDs-based device, and wavelengths and energy were the same among different light treatments. Doses of UV-C light that both effectively stimulated plant defences and were innocuous were determined beforehand. Tomato plants and lettuce plants were inoculated with Botrytis cinerea, pepper plants with Phytophthora capsici, and grapevine with Plasmopara viticola. In some experiments we investigated the effect of a repetition of treatments over periods of several days. All plants were inoculated 48 h after exposure to the last UV-C treatment. Lesions on surfaces were measured up to 12 days after inoculation, depending on the experiment and the pathogen. The results confirmed that UV-C light stimulates plant resistance; they show that irradiation for one second is more effective than irradiation for 60 s, and that repetition of treatments is more effective than single light treatments. Moreover a systemic effect was observed in unexposed leaves that were close to exposed leaves. The mechanisms of perception and of the signalling and metabolic pathways triggered by flashes of UV-C light vs. 60 s irradiation exposures are briefly discussed, as well as the prospects for field use of UV-C flashes in viticulture and horticulture.

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Section: Uv-c Light Stimulates Plant Defencessupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Flashes of UV-C light: An innovative method for stimulating plant defences

Aarrouf

Urban

2020

PLoS ONE

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“…On growing lettuce, Vàsquez et al () applied a 0.85 kJ m −2 dose four times, at 2‐day intervals, and found an increase in the total phenol content and in FaPAL , CAT, and MDAHR activities. The defence‐related genes coding for enzymes of the phenylpropanoid pathway (PAL and stilbene synthase), the octadecanoid pathway (lipoxygenase), and the PR proteins (CHI, BG2‐1, and BG2‐3) were also up‐regulated by UV‐C exposure in grapevine (Bonomelli et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…In general, these studies describe the effect of UV‐C attenuating disease development in the context of postharvest treatment of crops. Limited data are available on the responses of intact growing plants to this abiotic stressor with some recent studies that have focused on UV‐C‐induced disease resistance in growing crops (Janisiewicz, Takeda, Glenn, Camp, & Jurick, ; Vàsquez et al, ). The mechanisms underlying the interaction between preharvest UV‐C‐treated plants and pathogenic microorganisms are not well understood at present.…”

Section: Introductionmentioning

confidence: 99%

Ultraviolet‐C priming of strawberry leaves against subsequent Mycosphaerella fragariae infection involves the action of reactive oxygen species, plant hormones, and terpenes

Charles

Luo

et al. 2019

Plant Cell & Environment

169

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Ultraviolet-C (UV-C) radiation has been reported to induce defence responses to pathogens in growing crops and described as a new environmentally friendly method for disease control. However, whether the effect of the induced defence mechanisms will persist after the stress imposed by UV-C is alleviated and how these mechanisms interact with pathogen elicitors upon infection have not yet been investigated. Thus, we inoculated strawberry plants with Mycosphaerella fragariae, the causal agent of leaf spot disease, after 5 weeks of repeated UV-C irradiation treatment (cumulative dose of 10.2 kJ m −2 ) and investigated the alteration of gene expression and biochemical phenotypes. The results revealed that UV-C treatment had a significant impact on gene expression in strawberry leaves and led to the overexpression of a set of genes involved in plant-pathogen interaction. UV-C-treated leaves displayed a stronger response to infection after inoculation, with reduced symptoms and increases in accumulation of total phenolics and volatile terpenes, higher expression of pathogenesis-related proteins and the activity of several defence enzymes. This study presumptively describe, for the first time, the involvement of terpenes, reactive oxygen species, and abscisic acid, salicylic acid, jasmonic acid, and their transduction factors, in the network underpinning UV-C priming of growing crops for improved protection against pathogens.

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“…Loose-leaf lettuce is one of the most widely consumed vegetables in the world, owing to its high concentration of vitamins, fibre, minerals and other nutrients, its taste and crispy mouthfeel, and its high water concentration. 1,2 However, lettuce is prone to deterioration due to its high water concentration and tender texture. Currently, the methods most commonly used to preserve lettuce are physical, chemical and biochemical technologies, including low temperature and preservatives.…”

Section: Introductionmentioning

confidence: 99%

iTRAQ proteomics reveals changes in the lettuce (Lactuca sativa L. Grand Rapid) proteome related to colour and senescence under modified atmosphere packaging

Chen

Fan

et al. 2018

J Sci Food Agric

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BACKGROUND This study used the isobaric tags for relative and absolute quantitation (iTRAQ) technique to determine the effects of active modified atmosphere packaging (MAP) on the modulation of lettuce (Lactuca sativa L. Grand Rapid) colour and senescence. Lettuces, treated or not treated (control) with MAP, were placed in a refrigerator (4 °C, 90–95% relative humidity) for analysis at 3‐day intervals until the ninth day. RESULTS MAP treatment could suppress weight loss, loss of green colour and brittleness, and browning of lettuce; at the same time, MAP preserved the green colour of lettuce by inhibiting chlorophyll degradation and chloroplast disruption, and delayed lettuce senescence by inhibiting malondialdehyde (MDA) generation and increasing antioxidant enzyme activity. In total, 285 differentially abundant proteins (144 upregulated proteins and 141 downregulated proteins) were identified in MAP‐treated and non‐treated lettuce. Further analysis revealed that MAP regulated the expression of proteins involved in photosynthesis (e.g. pheophorbide a oxygenase, PaO; porphobilinogen deaminase, PBGD), and thus preserved lettuce colour. The iTRAQ analysis also showed that MAP regulated the expression of antioxidant enzymes and reduced the accumulation of reactive oxygen species, and thus delayed lettuce senescence. CONCLUSION MAP can maintain vegetable quality by inhibiting chlorophyll degradation, delaying vegetable senescence. © 2018 Society of Chemical Industry

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Pre-harvest hormetic doses of UV-C radiation can decrease susceptibility of lettuce leaves ( Lactuca sativa L.) to Botrytis cinerea L.

Cited by 45 publications

References 56 publications

Flashes of UV-C light: An innovative method for stimulating plant defences

Flashes of UV-C light: An innovative method for stimulating plant defences

Ultraviolet‐C priming of strawberry leaves against subsequent Mycosphaerella fragariae infection involves the action of reactive oxygen species, plant hormones, and terpenes

iTRAQ proteomics reveals changes in the lettuce (Lactuca sativa L. Grand Rapid) proteome related to colour and senescence under modified atmosphere packaging

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