Pre‐culturing human adipose tissue mesenchymal stem cells under hypoxia increases their adipogenic and osteogenic differentiation potentials

Valorani, M. G.; Montelatici, Elisa; Germani, Antonia; Biddle, Adrian; D’Alessandro, Delfo; Strollo, Rocky; Patrizi, Mp; Lazzari, Lorenza; Nye, Emma; Otto, Wr; Pozzilli, Paolo; Alison, Malcolm R.

doi:10.1111/j.1365-2184.2012.00817.x

Cited by 127 publications

(112 citation statements)

References 54 publications

(71 reference statements)

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“…However, one could be concerned that culturing MSCs under hypoxia might change their behaviour. Valorani et al [109] reported that adipose tissue-derived MSCs cultured under hypoxic conditions exhibited an increased adipogenic or osteogenic differentiation capacity [109] . Crowder et al [110] reported that concurrent exposure to extreme hypoxia (0.5%) and a carcinogenic metal (nickel) induces carcinogenic changes in late passage MSCs.…”

Section: Pretreatment or Priming Of Mscs In Culture Or Modifying The mentioning

confidence: 99%

Homing and migration of mesenchymal stromal cells: How to improve the efficacy of cell therapy?

Becker¹,

Riet²

2016

WJSC

386

313

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Mesenchymal stromal cells (MSCs) are currently being investigated for use in a wide variety of clinical applications. For most of these applications, systemic delivery of the cells is preferred. However, this requires the homing and migration of MSCs to a target tissue. Although MSC homing has been described, this process does not appear to be highly efficacious because only a few cells reach the target tissue and remain there after systemic administration. This has been ascribed to low expression levels of homing molecules, the loss of expression of such molecules during expansion, and the heterogeneity of MSCs in cultures and MSC culture protocols. To overcome these limitations, different methods to improve the homing capacity of MSCs have been examined. Here, we review the current understanding of MSC homing, with a particular focus on homing to bone marrow. In addition, we summarize the strategies that have been developed to improve this process. A better understanding of MSC biology, MSC migration and homing mechanisms will allow us to prepare MSCs with optimal homing capacities. The efficacy of therapeutic applications is dependent on efficient delivery of the cells and can, therefore, only benefit from better insights into the homing mechanisms.

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Section: Pretreatment or Priming Of Mscs In Culture Or Modifying The mentioning

confidence: 99%

Homing and migration of mesenchymal stromal cells: How to improve the efficacy of cell therapy?

Becker¹,

Riet²

2016

WJSC

386

313

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“…Preculturing of MSCs in hypoxic conditions has demonstrated improved angiogenic function once transferred into an in vivo environment. [19][20][21] MSCs were shown to have enhanced migration rates and a number of upregulated growth factors and corresponding receptors, such as hepatocyte growth factor, which is responsible for MSC recruitment to damaged and ischemic tissues. 19 These results demonstrate the sensitivity of MSCs to their culturing conditions and the potential to fine-tune their environment to enhance vascularization in tissue constructs as well as in vivo transplantations.…”

Section: Hypoxiamentioning

confidence: 99%

Mesenchymal Stem Cells: Roles and Relationships in Vascularization

Melchiorri

Nguyen

Fisher

2014

Tissue Engineering Part B: Reviews

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“…It has been demonstrated that hMSC at 20% O 2 exhibited significantly increased oxidative stress, DNA damage markers, and telomere shortening rates, compared with cells grown at 3% O 2 [15]. Conversely, numerous studies have demonstrated that ASCs under hypoxia showed the greater number of cells and shorter population doubling time, and oxygen tension as low as 2% had the greatest impact on cell growth [16][17][18]. For clinical applications, stem cells must be sufficiently expanded.…”

Section: Stem Cell Proliferation In Vitromentioning

confidence: 99%

Approaches for Stimulating Proliferation of Stem Cells in Vitro

Liu¹,

Li²,

Cai³

2017

JAMB

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Abstract.With the increasing incidence rates in the complex human diseases, e.g., Alzheimer's disease, spinal cord injury, and diabetes, there exists an urgent need to search for more effective and efficient therapies. Cell-based therapy has been considered to be a novel and effective therapeutic approach for the great potential of stem cells in their self-renewal and multipotential differentiational capacity. However, a major difficulty with the success of stem cell therapy is the availability of stem cells. The most serious issue is that only a limited number of stem cells can be extracted from adult tissue due to the decreasing frequency and differentiation potential with age. In order to achieve successful cell-based therapy, extensive ex vivo proliferation of stem cells is urgently required. Therefore, approaches to stimulate proliferation of stem cells are discussed in this article.

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Pre‐culturing human adipose tissue mesenchymal stem cells under hypoxia increases their adipogenic and osteogenic differentiation potentials

Abstract: This protocol could be useful for maximizing hAT-MSC potential to differentiate in vitro into the adipogenic and osteogenic lineages, for use in plastic and reconstructive surgery, and in tissue engineering strategies.

Cited by 127 publications

References 54 publications

Homing and migration of mesenchymal stromal cells: How to improve the efficacy of cell therapy?

Homing and migration of mesenchymal stromal cells: How to improve the efficacy of cell therapy?

Mesenchymal Stem Cells: Roles and Relationships in Vascularization

Approaches for Stimulating Proliferation of Stem Cells in Vitro

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