Pre-column incubation followed by fast liquid chromatography analysis for rapid screening of natural methylglyoxal scavengers directly from herbal medicines: Case study of Polygonum cuspidatum

Tang, Dan; Zhu, Jiaqian; Wu, Anguo; Xu, Youhua; Duan, Tingting; Zheng, Zong‐Ping; Wang, Ru-Shang; Li, Dan; Zhu, Quan

doi:10.1016/j.chroma.2013.02.058

Cited by 27 publications

(21 citation statements)

References 43 publications

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“…Naphtolic derivatives including torachrysone 8-O-β-D-glucopyranoside (2) have been identified in the Polygonaceae [3,[8][9][10][11][12] and other families such as Fabaceae, Rhamnaceae, Araliaceae, and Euphorbiaceae [13][14][15][16]. All those compounds possessed a hydroxyl group at C-1, an acetyl group at C-2, and a methyl at C-3 in the naphthalene skeleton.…”

Section: Resultsmentioning

confidence: 99%

Phenolic Constituents from Fallopia multiflora (Thunberg) Haraldson

Nguyen

Pham²,

Thao³

et al. 2018

Journal of Chemistry

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Four naphtolic glycosides (1–4), three anthraquinones (5–7), two stilbenes (8-9), one benzyl glycoside (10), and one flavonoid (11) were isolated from the roots of Fallopia multiflora. The new compounds were elucidated to be 6-hydroxymusizin 8-O-α-D-apiofuranosyl-(1→6)-β-D-glucopyranoside (1) and 6-methoxy-3-methyl-1,6,8-trihydroxy-2-naphthoic acid 8-O-β-D-glucopyranoside (3). Compound 3 could be an important marker for chemotaxonomy of F. multiflora. Benzyl gentiobioside (10) was isolated for the first time from the family Polygonaceae.

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Section: Resultsmentioning

confidence: 99%

Phenolic Constituents from Fallopia multiflora (Thunberg) Haraldson

Nguyen

Pham²,

Thao³

et al. 2018

Journal of Chemistry

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“…The identities of the 11 peaks were confirmed by the comparison of retention times and MS/MS data with that of available references compounds or that in literatures [16][17][18][19][20]. The chromatogram (UHPLC) of Rhizoma Polygoni Cuspidati is shown in Fig.3.…”

Section: Identification Of Rhizoma Polygoni Cuspidati Extractmentioning

confidence: 97%

Chinese hamster ovary‐sphingomyelin synthase2 biospecific extraction and liquid chromatography with tandem mass spectrometry analysis for the prediction of bioactive components of Rhizoma Polygoni Cuspidati

Xue

Dong

Liang

2016

J of Separation Science

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A novel strategy for predicting bioactive components in traditional Chinese medicines using Chinese hamster ovary-sphingomyelin synthase2 (CHO-SMS2 ) cell biospecific extraction and high-performance liquid chromatography with diode array detection and tandem mass spectrometry analysis was proposed. The hypothesis is that when cells are incubated with the extract of traditional Chinese medicines, the potential bioactive components in the traditional Chinese medicines should selectively combine with the cells, while the cell-combining components would be detectable in the extract of denatured cells. The identities of the cell-combining components could be determined by liquid chromatography with tandem mass spectrometry. Using the proposed approach, the potential bioactive components of Rhizoma Polygoni Cuspidati, a commonly used traditional Chinese medicine for atherosclerosis, were detected and identified. Eight compounds in the extract of Rhizoma Polygoni Cuspidati were detected as the components selectively combined with CHO-SMS2 cells, which is a stable cell line that highly expresses sphingomyelin synthases, it was found that piceid, resveratrol, emodin-8-β-d-glucoside, physcion-8-β-d-glucoside, emodin, physcion, 3,5,4'-trihydroxystilbene-3-O-(6"-galloyl)-glucoside, and emodin-1-O-glucoside combined specifically with CHO-SMS2 cells. The results indicate that the proposed approach may be applied to predict the bioactive candidates in traditional Chinese medicines.

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“…Methylglyoxal is a highly reactive metabolite of glucose that has been implicated in several chronic diseases associated with diabetes [150,151]. The elevated concentrations of methylglyoxal in diabetes patients can also lead to protein modification and the formation of AGEs through the reaction of methylglyoxal with arginine or lysine residues.…”

Section: Variations In Protein Structure and Binding Due To Metabomentioning

confidence: 99%

“…The elevated concentrations of methylglyoxal in diabetes patients can also lead to protein modification and the formation of AGEs through the reaction of methylglyoxal with arginine or lysine residues. A recent report using quantitative MS and multiple reaction monitoring found that a major site for modification by methylglyoxal on HSA occurs at Arg-257, which is located in Sudlow site I. Molecular modeling conducted in the same study indicated that a decrease in binding by warfarin may occur due to these modifications when comparing glycated HSA and normal HSA [151]. …”

Section: Variations In Protein Structure and Binding Due To Metabomentioning

confidence: 99%

Studies of metabolite–protein interactions: A review

Matsuda

Anguizola

et al. 2014

Journal of Chromatography B

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The study of metabolomics can provide valuable information about biochemical pathways and processes at the molecular level. There have been many reports that have examined the structure, identity and concentrations of metabolites in biological systems. However, the binding of metabolites with proteins is also of growing interest. This review examines past reports that have looked at the binding of various types of metabolites with proteins. An overview of the techniques that have been used to characterize and study metabolite-protein binding is first provided. This is followed by examples of studies that have investigated the binding of hormones, fatty acids, drugs or other xenobiotics, and their metabolites with transport proteins and receptors. These examples include reports that have considered the structure of the resulting solute-protein complexes, the nature of the binding sites, the strength of these interactions, the variations in these interactions with solute structure, and the kinetics of these reactions. The possible effects of metabolic diseases on these processes, including the impact of alterations in the structure and function of proteins, are also considered.

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Pre-column incubation followed by fast liquid chromatography analysis for rapid screening of natural methylglyoxal scavengers directly from herbal medicines: Case study of Polygonum cuspidatum

Cited by 27 publications

References 43 publications

Phenolic Constituents from Fallopia multiflora (Thunberg) Haraldson

Phenolic Constituents from Fallopia multiflora (Thunberg) Haraldson

Chinese hamster ovary‐sphingomyelin synthase2 biospecific extraction and liquid chromatography with tandem mass spectrometry analysis for the prediction of bioactive components of Rhizoma Polygoni Cuspidati

Studies of metabolite–protein interactions: A review

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